Strain Fitness in Escherichia coli BW25113 around b4070

Experiment: ethyl acetate 5 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntacs and nrfA are separated by 392 nucleotidesnrfA and nrfB are separated by 44 nucleotidesnrfB and nrfC overlap by 4 nucleotides b4069: acs - acetyl-coenzyme A synthetase (NCBI), at 4,283,436 to 4,285,394 acs b4070: nrfA - nitrite reductase, formate-dependent, cytochrome (NCBI), at 4,285,787 to 4,287,223 nrfA b4071: nrfB - formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS), at 4,287,268 to 4,287,834 nrfB b4072: nrfC - formate-dependent nitrite reductase, 4Fe4S subunit (NCBI), at 4,287,831 to 4,288,502 nrfC Position (kb) 4285 4286 4287 4288Strain fitness (log2 ratio) -1 0 1 2at 4284.792 kb on - strand, within acsat 4284.811 kb on - strand, within acsat 4284.811 kb on - strand, within acsat 4284.841 kb on + strand, within acsat 4284.983 kb on - strand, within acsat 4285.188 kb on + strand, within acsat 4285.199 kb on + strandat 4285.326 kb on - strandat 4285.326 kb on - strandat 4285.406 kb on + strandat 4285.695 kb on + strandat 4285.711 kb on + strandat 4285.896 kb on + strandat 4285.948 kb on + strand, within nrfAat 4285.948 kb on + strand, within nrfAat 4285.967 kb on + strand, within nrfAat 4286.025 kb on - strand, within nrfAat 4286.025 kb on - strand, within nrfAat 4286.035 kb on + strand, within nrfAat 4286.035 kb on - strand, within nrfAat 4286.089 kb on + strand, within nrfAat 4286.089 kb on + strand, within nrfAat 4286.107 kb on - strand, within nrfAat 4286.107 kb on - strand, within nrfAat 4286.109 kb on - strand, within nrfAat 4286.135 kb on + strand, within nrfAat 4286.135 kb on + strand, within nrfAat 4286.168 kb on - strand, within nrfAat 4286.168 kb on - strand, within nrfAat 4286.179 kb on - strand, within nrfAat 4286.340 kb on - strand, within nrfAat 4286.419 kb on + strand, within nrfAat 4286.419 kb on + strand, within nrfAat 4286.427 kb on - strand, within nrfAat 4286.427 kb on - strand, within nrfAat 4286.526 kb on + strand, within nrfAat 4286.526 kb on + strand, within nrfAat 4286.590 kb on + strand, within nrfAat 4286.629 kb on - strand, within nrfAat 4286.629 kb on - strand, within nrfAat 4286.674 kb on + strand, within nrfAat 4286.682 kb on - strand, within nrfAat 4286.846 kb on + strand, within nrfAat 4286.954 kb on + strand, within nrfAat 4286.962 kb on - strand, within nrfAat 4286.965 kb on - strand, within nrfAat 4286.965 kb on - strand, within nrfAat 4287.004 kb on + strand, within nrfAat 4287.058 kb on - strand, within nrfAat 4287.119 kb on + strandat 4287.149 kb on + strandat 4287.223 kb on + strandat 4287.250 kb on + strandat 4287.250 kb on + strandat 4287.250 kb on + strandat 4287.258 kb on - strandat 4287.262 kb on + strandat 4287.301 kb on + strandat 4287.301 kb on + strandat 4287.308 kb on + strandat 4287.313 kb on + strandat 4287.313 kb on + strandat 4287.328 kb on - strand, within nrfBat 4287.534 kb on - strand, within nrfBat 4287.560 kb on + strand, within nrfBat 4287.560 kb on + strand, within nrfBat 4287.582 kb on - strand, within nrfBat 4287.591 kb on + strand, within nrfBat 4287.619 kb on - strand, within nrfBat 4287.718 kb on + strand, within nrfBat 4287.823 kb on - strandat 4287.823 kb on - strandat 4287.836 kb on - strandat 4287.836 kb on - strandat 4287.905 kb on - strand, within nrfCat 4287.970 kb on - strand, within nrfCat 4287.981 kb on - strand, within nrfCat 4287.985 kb on + strand, within nrfCat 4288.125 kb on - strand, within nrfCat 4288.153 kb on - strand, within nrfCat 4288.182 kb on + strand, within nrfCat 4288.182 kb on + strand, within nrfCat 4288.212 kb on + strand, within nrfCat 4288.212 kb on + strand, within nrfCat 4288.220 kb on - strand, within nrfC

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Per-strain Table

Position Strand Gene LocusTag Fraction ethyl acetate 5 vol%
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4,284,792 - acs b4069 0.69 -0.6
4,284,811 - acs b4069 0.70 +0.7
4,284,811 - acs b4069 0.70 +0.3
4,284,841 + acs b4069 0.72 -0.0
4,284,983 - acs b4069 0.79 -0.1
4,285,188 + acs b4069 0.89 +1.4
4,285,199 + -0.0
4,285,326 - +0.3
4,285,326 - +0.1
4,285,406 + +0.3
4,285,695 + -0.7
4,285,711 + -0.2
4,285,896 + +0.2
4,285,948 + nrfA b4070 0.11 -0.4
4,285,948 + nrfA b4070 0.11 +0.2
4,285,967 + nrfA b4070 0.13 -0.1
4,286,025 - nrfA b4070 0.17 -0.1
4,286,025 - nrfA b4070 0.17 -1.0
4,286,035 + nrfA b4070 0.17 +0.6
4,286,035 - nrfA b4070 0.17 +0.1
4,286,089 + nrfA b4070 0.21 -0.3
4,286,089 + nrfA b4070 0.21 +0.2
4,286,107 - nrfA b4070 0.22 -0.6
4,286,107 - nrfA b4070 0.22 +0.4
4,286,109 - nrfA b4070 0.22 +1.0
4,286,135 + nrfA b4070 0.24 -0.6
4,286,135 + nrfA b4070 0.24 -0.2
4,286,168 - nrfA b4070 0.27 +0.5
4,286,168 - nrfA b4070 0.27 +0.1
4,286,179 - nrfA b4070 0.27 -0.8
4,286,340 - nrfA b4070 0.38 +0.4
4,286,419 + nrfA b4070 0.44 -0.7
4,286,419 + nrfA b4070 0.44 +2.4
4,286,427 - nrfA b4070 0.45 -0.7
4,286,427 - nrfA b4070 0.45 +0.2
4,286,526 + nrfA b4070 0.51 -0.3
4,286,526 + nrfA b4070 0.51 -0.4
4,286,590 + nrfA b4070 0.56 +0.1
4,286,629 - nrfA b4070 0.59 -0.6
4,286,629 - nrfA b4070 0.59 -0.1
4,286,674 + nrfA b4070 0.62 +0.6
4,286,682 - nrfA b4070 0.62 -0.5
4,286,846 + nrfA b4070 0.74 -0.0
4,286,954 + nrfA b4070 0.81 +0.6
4,286,962 - nrfA b4070 0.82 +1.1
4,286,965 - nrfA b4070 0.82 -0.1
4,286,965 - nrfA b4070 0.82 +1.7
4,287,004 + nrfA b4070 0.85 +1.4
4,287,058 - nrfA b4070 0.88 -0.1
4,287,119 + -0.1
4,287,149 + -0.4
4,287,223 + +0.3
4,287,250 + -0.3
4,287,250 + +0.1
4,287,250 + +2.0
4,287,258 - -0.1
4,287,262 + -1.4
4,287,301 + +0.2
4,287,301 + -0.2
4,287,308 + +0.3
4,287,313 + +0.0
4,287,313 + +0.2
4,287,328 - nrfB b4071 0.11 +0.3
4,287,534 - nrfB b4071 0.47 -0.9
4,287,560 + nrfB b4071 0.51 +1.4
4,287,560 + nrfB b4071 0.51 +1.5
4,287,582 - nrfB b4071 0.55 +0.3
4,287,591 + nrfB b4071 0.57 -0.9
4,287,619 - nrfB b4071 0.62 -0.8
4,287,718 + nrfB b4071 0.79 -1.3
4,287,823 - +0.2
4,287,823 - +0.4
4,287,836 - +0.4
4,287,836 - -0.4
4,287,905 - nrfC b4072 0.11 +0.0
4,287,970 - nrfC b4072 0.21 +0.4
4,287,981 - nrfC b4072 0.22 +0.2
4,287,985 + nrfC b4072 0.23 +0.6
4,288,125 - nrfC b4072 0.44 +0.2
4,288,153 - nrfC b4072 0.48 +0.6
4,288,182 + nrfC b4072 0.52 -0.7
4,288,182 + nrfC b4072 0.52 +0.3
4,288,212 + nrfC b4072 0.57 -0.5
4,288,212 + nrfC b4072 0.57 -0.3
4,288,220 - nrfC b4072 0.58 +0.3

Or see this region's nucleotide sequence