Experiment: ethyl acetate 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yadG and yadH overlap by 4 nucleotides yadH and yadI are separated by 104 nucleotides yadI and yadE are separated by 63 nucleotides
b0127: yadG - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 142,779 to 143,705
yadG
b0128: yadH - predicted transporter subunit: membrane component of ABC superfamily (NCBI), at 143,702 to 144,472
yadH
b0129: yadI - predicted PTS Enzyme IIA (NCBI), at 144,577 to 145,017
yadI
b0130: yadE - predicted polysaccharide deacetylase lipoprotein (NCBI), at 145,081 to 146,310
yadE
Position (kb) 144
145
146 Strain fitness (log2 ratio) -1
0
1
2 at 143.585 kb on - strand, within yadG at 143.590 kb on + strand, within yadG at 143.590 kb on + strand, within yadG at 143.607 kb on - strand, within yadG at 143.620 kb on - strand at 143.621 kb on + strand at 143.744 kb on + strand at 143.757 kb on + strand at 143.782 kb on - strand, within yadH at 143.790 kb on + strand, within yadH at 143.815 kb on + strand, within yadH at 143.824 kb on + strand, within yadH at 143.845 kb on + strand, within yadH at 143.879 kb on + strand, within yadH at 143.899 kb on + strand, within yadH at 143.912 kb on + strand, within yadH at 143.986 kb on + strand, within yadH at 144.160 kb on - strand, within yadH at 144.218 kb on + strand, within yadH at 144.256 kb on - strand, within yadH at 144.302 kb on + strand, within yadH at 144.304 kb on + strand, within yadH at 144.304 kb on + strand, within yadH at 144.331 kb on + strand, within yadH at 144.333 kb on + strand, within yadH at 144.386 kb on + strand, within yadH at 144.386 kb on + strand, within yadH at 144.403 kb on + strand at 144.403 kb on + strand at 144.440 kb on - strand at 144.539 kb on + strand at 144.539 kb on + strand at 144.539 kb on + strand at 144.539 kb on + strand at 144.630 kb on - strand, within yadI at 144.630 kb on - strand, within yadI at 144.663 kb on + strand, within yadI at 144.663 kb on + strand, within yadI at 144.757 kb on + strand, within yadI at 144.772 kb on + strand, within yadI at 144.873 kb on + strand, within yadI at 144.881 kb on - strand, within yadI at 144.885 kb on + strand, within yadI at 144.910 kb on - strand, within yadI at 145.116 kb on + strand at 145.136 kb on - strand at 145.136 kb on - strand at 145.143 kb on + strand at 145.171 kb on - strand at 145.171 kb on - strand at 145.237 kb on + strand, within yadE at 145.237 kb on + strand, within yadE at 145.325 kb on + strand, within yadE at 145.355 kb on + strand, within yadE at 145.362 kb on - strand, within yadE at 145.362 kb on - strand, within yadE at 145.518 kb on - strand, within yadE at 145.530 kb on + strand, within yadE at 145.549 kb on + strand, within yadE at 145.584 kb on - strand, within yadE at 145.584 kb on - strand, within yadE at 145.594 kb on + strand, within yadE at 145.594 kb on + strand, within yadE at 145.732 kb on - strand, within yadE at 145.810 kb on - strand, within yadE at 145.811 kb on - strand, within yadE at 145.873 kb on - strand, within yadE at 145.945 kb on - strand, within yadE at 145.945 kb on - strand, within yadE
Per-strain Table
Position Strand Gene LocusTag Fraction ethyl acetate 5 vol% remove 143,585 - yadG b0127 0.87 +0.6 143,590 + yadG b0127 0.87 -0.6 143,590 + yadG b0127 0.87 +0.4 143,607 - yadG b0127 0.89 +0.3 143,620 - +0.3 143,621 + -0.9 143,744 + +0.0 143,757 + +0.1 143,782 - yadH b0128 0.10 +0.4 143,790 + yadH b0128 0.11 -0.1 143,815 + yadH b0128 0.15 -1.1 143,824 + yadH b0128 0.16 +0.2 143,845 + yadH b0128 0.19 -0.4 143,879 + yadH b0128 0.23 +0.5 143,899 + yadH b0128 0.26 -0.1 143,912 + yadH b0128 0.27 +0.2 143,986 + yadH b0128 0.37 -0.1 144,160 - yadH b0128 0.59 -0.1 144,218 + yadH b0128 0.67 +0.0 144,256 - yadH b0128 0.72 -0.2 144,302 + yadH b0128 0.78 -0.3 144,304 + yadH b0128 0.78 +0.1 144,304 + yadH b0128 0.78 +1.2 144,331 + yadH b0128 0.82 +0.2 144,333 + yadH b0128 0.82 +0.3 144,386 + yadH b0128 0.89 +2.2 144,386 + yadH b0128 0.89 -0.4 144,403 + -0.0 144,403 + -1.4 144,440 - +0.6 144,539 + -0.1 144,539 + -1.1 144,539 + -0.0 144,539 + -0.0 144,630 - yadI b0129 0.12 +0.4 144,630 - yadI b0129 0.12 -0.1 144,663 + yadI b0129 0.20 -0.5 144,663 + yadI b0129 0.20 +0.6 144,757 + yadI b0129 0.41 -0.0 144,772 + yadI b0129 0.44 +0.5 144,873 + yadI b0129 0.67 -0.4 144,881 - yadI b0129 0.69 +1.0 144,885 + yadI b0129 0.70 -0.1 144,910 - yadI b0129 0.76 -0.5 145,116 + -0.3 145,136 - +0.8 145,136 - -0.3 145,143 + +0.4 145,171 - -0.3 145,171 - +0.4 145,237 + yadE b0130 0.13 +0.6 145,237 + yadE b0130 0.13 +0.4 145,325 + yadE b0130 0.20 +0.2 145,355 + yadE b0130 0.22 +0.6 145,362 - yadE b0130 0.23 -0.3 145,362 - yadE b0130 0.23 +0.8 145,518 - yadE b0130 0.36 -0.8 145,530 + yadE b0130 0.37 +0.4 145,549 + yadE b0130 0.38 +1.1 145,584 - yadE b0130 0.41 -1.2 145,584 - yadE b0130 0.41 +0.3 145,594 + yadE b0130 0.42 -1.1 145,594 + yadE b0130 0.42 -0.5 145,732 - yadE b0130 0.53 -0.7 145,810 - yadE b0130 0.59 +0.3 145,811 - yadE b0130 0.59 +0.3 145,873 - yadE b0130 0.64 +0.5 145,945 - yadE b0130 0.70 -0.9 145,945 - yadE b0130 0.70 -0.6
Or see this region's nucleotide sequence