Strain Fitness in Escherichia coli BW25113 around b3232

Experiment: ethyl acetate 5 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrpsI and rplM are separated by 15 nucleotidesrplM and yhcM are separated by 218 nucleotidesyhcM and yhcB are separated by 193 nucleotidesyhcB and degQ are separated by 153 nucleotides b3230: rpsI - 30S ribosomal protein S9 (NCBI), at 3,375,837 to 3,376,229 rpsI b3231: rplM - 50S ribosomal protein L13 (NCBI), at 3,376,245 to 3,376,673 rplM b3232: yhcM - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 3,376,892 to 3,378,019 yhcM b3233: yhcB - orf, hypothetical protein (VIMSS), at 3,378,213 to 3,378,611 yhcB b3234: degQ - serine endoprotease, periplasmic (NCBI), at 3,378,765 to 3,380,132 degQ Position (kb) 3376 3377 3378 3379Strain fitness (log2 ratio) -2 -1 0 1 2at 3376.719 kb on - strandat 3376.719 kb on - strandat 3376.844 kb on - strandat 3376.845 kb on - strandat 3376.929 kb on - strandat 3377.108 kb on + strand, within yhcMat 3377.108 kb on + strand, within yhcMat 3377.130 kb on - strand, within yhcMat 3377.162 kb on - strand, within yhcMat 3377.182 kb on - strand, within yhcMat 3377.191 kb on + strand, within yhcMat 3377.191 kb on + strand, within yhcMat 3377.193 kb on + strand, within yhcMat 3377.193 kb on + strand, within yhcMat 3377.283 kb on + strand, within yhcMat 3377.296 kb on - strand, within yhcMat 3377.296 kb on - strand, within yhcMat 3377.299 kb on + strand, within yhcMat 3377.375 kb on - strand, within yhcMat 3377.375 kb on - strand, within yhcMat 3377.375 kb on - strand, within yhcMat 3377.375 kb on - strand, within yhcMat 3377.375 kb on - strand, within yhcMat 3377.375 kb on - strand, within yhcMat 3377.412 kb on + strand, within yhcMat 3377.412 kb on + strand, within yhcMat 3377.514 kb on + strand, within yhcMat 3377.516 kb on - strand, within yhcMat 3377.520 kb on - strand, within yhcMat 3377.520 kb on - strand, within yhcMat 3377.651 kb on + strand, within yhcMat 3377.652 kb on + strand, within yhcMat 3377.652 kb on + strand, within yhcMat 3377.659 kb on + strand, within yhcMat 3377.668 kb on - strand, within yhcMat 3377.668 kb on - strand, within yhcMat 3377.677 kb on + strand, within yhcMat 3377.677 kb on + strand, within yhcMat 3377.700 kb on - strand, within yhcMat 3377.800 kb on - strand, within yhcMat 3377.848 kb on - strand, within yhcMat 3377.848 kb on - strand, within yhcMat 3377.853 kb on - strand, within yhcMat 3377.853 kb on - strand, within yhcMat 3377.900 kb on - strand, within yhcMat 3377.900 kb on - strand, within yhcMat 3377.903 kb on - strand, within yhcMat 3377.903 kb on - strand, within yhcMat 3377.960 kb on - strandat 3377.977 kb on - strandat 3377.980 kb on - strandat 3377.980 kb on - strandat 3378.083 kb on + strandat 3378.091 kb on - strandat 3378.091 kb on - strandat 3378.091 kb on - strandat 3378.158 kb on + strandat 3378.158 kb on + strandat 3378.166 kb on + strandat 3378.173 kb on + strandat 3378.173 kb on + strandat 3378.188 kb on - strandat 3378.532 kb on + strandat 3378.584 kb on + strandat 3378.640 kb on + strandat 3378.654 kb on + strandat 3378.693 kb on - strandat 3378.711 kb on + strandat 3378.731 kb on + strandat 3378.783 kb on + strandat 3378.798 kb on + strandat 3378.798 kb on + strandat 3378.824 kb on - strandat 3378.825 kb on + strandat 3378.858 kb on - strandat 3378.858 kb on - strandat 3378.881 kb on + strandat 3378.898 kb on - strandat 3378.928 kb on + strand, within degQat 3378.936 kb on - strand, within degQat 3378.952 kb on + strand, within degQ

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Per-strain Table

Position Strand Gene LocusTag Fraction ethyl acetate 5 vol%
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3,376,719 - +0.6
3,376,719 - -0.7
3,376,844 - -1.9
3,376,845 - +0.2
3,376,929 - -0.3
3,377,108 + yhcM b3232 0.19 -1.4
3,377,108 + yhcM b3232 0.19 -0.1
3,377,130 - yhcM b3232 0.21 -0.9
3,377,162 - yhcM b3232 0.24 -0.7
3,377,182 - yhcM b3232 0.26 -1.9
3,377,191 + yhcM b3232 0.27 -1.5
3,377,191 + yhcM b3232 0.27 -1.5
3,377,193 + yhcM b3232 0.27 -1.2
3,377,193 + yhcM b3232 0.27 -0.7
3,377,283 + yhcM b3232 0.35 -1.4
3,377,296 - yhcM b3232 0.36 -0.5
3,377,296 - yhcM b3232 0.36 -2.6
3,377,299 + yhcM b3232 0.36 +0.1
3,377,375 - yhcM b3232 0.43 -1.8
3,377,375 - yhcM b3232 0.43 -1.2
3,377,375 - yhcM b3232 0.43 -0.4
3,377,375 - yhcM b3232 0.43 -0.6
3,377,375 - yhcM b3232 0.43 +0.9
3,377,375 - yhcM b3232 0.43 -0.6
3,377,412 + yhcM b3232 0.46 -0.6
3,377,412 + yhcM b3232 0.46 -0.1
3,377,514 + yhcM b3232 0.55 -1.3
3,377,516 - yhcM b3232 0.55 -1.5
3,377,520 - yhcM b3232 0.56 -0.6
3,377,520 - yhcM b3232 0.56 +1.4
3,377,651 + yhcM b3232 0.67 -1.0
3,377,652 + yhcM b3232 0.67 -0.7
3,377,652 + yhcM b3232 0.67 +0.2
3,377,659 + yhcM b3232 0.68 -1.4
3,377,668 - yhcM b3232 0.69 -1.9
3,377,668 - yhcM b3232 0.69 -1.5
3,377,677 + yhcM b3232 0.70 -1.6
3,377,677 + yhcM b3232 0.70 -1.1
3,377,700 - yhcM b3232 0.72 -0.2
3,377,800 - yhcM b3232 0.80 -0.6
3,377,848 - yhcM b3232 0.85 -1.1
3,377,848 - yhcM b3232 0.85 +1.3
3,377,853 - yhcM b3232 0.85 -2.0
3,377,853 - yhcM b3232 0.85 -0.6
3,377,900 - yhcM b3232 0.89 -1.6
3,377,900 - yhcM b3232 0.89 -1.7
3,377,903 - yhcM b3232 0.90 -0.7
3,377,903 - yhcM b3232 0.90 -0.9
3,377,960 - -0.7
3,377,977 - -1.1
3,377,980 - -0.3
3,377,980 - +0.9
3,378,083 + -0.1
3,378,091 - +1.4
3,378,091 - +0.1
3,378,091 - -0.3
3,378,158 + -0.6
3,378,158 + +0.1
3,378,166 + -0.5
3,378,173 + +0.2
3,378,173 + +0.2
3,378,188 - -0.9
3,378,532 + +2.0
3,378,584 + -2.1
3,378,640 + +2.0
3,378,654 + +0.0
3,378,693 - +0.0
3,378,711 + +0.2
3,378,731 + +0.5
3,378,783 + -0.2
3,378,798 + +0.4
3,378,798 + -0.1
3,378,824 - +0.5
3,378,825 + -1.0
3,378,858 - -1.4
3,378,858 - +0.7
3,378,881 + -0.4
3,378,898 - -0.9
3,378,928 + degQ b3234 0.12 +0.1
3,378,936 - degQ b3234 0.12 +0.6
3,378,952 + degQ b3234 0.14 +0.3

Or see this region's nucleotide sequence