Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF724 and GFF725 overlap by 1 nucleotides GFF725 and GFF726 are separated by 202 nucleotides
GFF724 - Molybdopterin biosynthesis protein MoeB, at 82,026 to 82,775
GFF724
GFF725 - Molybdopterin biosynthesis protein MoeA, at 82,775 to 84,010
GFF725
GFF726 - Isoaspartyl aminopeptidase (EC 3.4.19.5) @ Asp-X dipeptidase, at 84,213 to 85,154
GFF726
Position (kb) 82
83
84
85 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 81.857 kb on - strand at 81.892 kb on - strand at 81.969 kb on - strand at 81.979 kb on - strand at 82.006 kb on + strand at 82.099 kb on + strand at 82.100 kb on - strand at 82.100 kb on - strand at 82.100 kb on - strand at 82.149 kb on - strand, within GFF724 at 82.283 kb on + strand, within GFF724 at 82.283 kb on + strand, within GFF724 at 82.330 kb on - strand, within GFF724 at 82.346 kb on + strand, within GFF724 at 82.349 kb on - strand, within GFF724 at 82.374 kb on + strand, within GFF724 at 82.374 kb on + strand, within GFF724 at 82.375 kb on - strand, within GFF724 at 82.434 kb on - strand, within GFF724 at 82.467 kb on - strand, within GFF724 at 82.475 kb on + strand, within GFF724 at 82.475 kb on + strand, within GFF724 at 82.515 kb on + strand, within GFF724 at 82.727 kb on + strand at 82.945 kb on + strand, within GFF725 at 83.137 kb on + strand, within GFF725 at 83.137 kb on + strand, within GFF725 at 83.138 kb on - strand, within GFF725 at 83.138 kb on - strand, within GFF725 at 83.138 kb on - strand, within GFF725 at 83.232 kb on + strand, within GFF725 at 83.232 kb on + strand, within GFF725 at 83.267 kb on + strand, within GFF725 at 83.267 kb on + strand, within GFF725 at 83.268 kb on - strand, within GFF725 at 83.336 kb on - strand, within GFF725 at 83.386 kb on + strand, within GFF725 at 83.387 kb on - strand, within GFF725 at 83.387 kb on - strand, within GFF725 at 83.482 kb on + strand, within GFF725 at 83.540 kb on - strand, within GFF725 at 83.950 kb on + strand at 84.017 kb on - strand at 84.017 kb on - strand at 84.045 kb on + strand at 84.048 kb on + strand at 84.049 kb on - strand at 84.049 kb on - strand at 84.049 kb on - strand at 84.073 kb on + strand at 84.110 kb on - strand at 84.114 kb on - strand at 84.119 kb on - strand at 84.131 kb on - strand at 84.133 kb on - strand at 84.174 kb on + strand at 84.174 kb on + strand at 84.289 kb on + strand at 84.290 kb on - strand at 84.295 kb on - strand at 84.367 kb on + strand, within GFF726 at 84.367 kb on + strand, within GFF726 at 84.432 kb on + strand, within GFF726 at 84.433 kb on - strand, within GFF726 at 84.571 kb on + strand, within GFF726 at 84.572 kb on - strand, within GFF726 at 84.819 kb on - strand, within GFF726 at 84.819 kb on - strand, within GFF726
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 81,857 - -0.1 81,892 - +0.3 81,969 - +0.1 81,979 - +1.1 82,006 + +0.3 82,099 + +0.8 82,100 - +0.2 82,100 - +0.6 82,100 - +0.5 82,149 - GFF724 0.16 +0.3 82,283 + GFF724 0.34 +0.9 82,283 + GFF724 0.34 +0.3 82,330 - GFF724 0.41 +0.7 82,346 + GFF724 0.43 +1.2 82,349 - GFF724 0.43 +0.6 82,374 + GFF724 0.46 -0.6 82,374 + GFF724 0.46 +0.4 82,375 - GFF724 0.47 +0.4 82,434 - GFF724 0.54 +0.4 82,467 - GFF724 0.59 -0.1 82,475 + GFF724 0.60 +1.2 82,475 + GFF724 0.60 -0.2 82,515 + GFF724 0.65 +0.4 82,727 + -0.1 82,945 + GFF725 0.14 +0.3 83,137 + GFF725 0.29 +0.9 83,137 + GFF725 0.29 +1.1 83,138 - GFF725 0.29 +0.2 83,138 - GFF725 0.29 +0.6 83,138 - GFF725 0.29 +0.2 83,232 + GFF725 0.37 +0.7 83,232 + GFF725 0.37 +0.4 83,267 + GFF725 0.40 +0.4 83,267 + GFF725 0.40 +0.3 83,268 - GFF725 0.40 +0.8 83,336 - GFF725 0.45 +0.8 83,386 + GFF725 0.49 +0.1 83,387 - GFF725 0.50 +1.2 83,387 - GFF725 0.50 +0.5 83,482 + GFF725 0.57 +1.1 83,540 - GFF725 0.62 +0.7 83,950 + +0.3 84,017 - +0.8 84,017 - +0.8 84,045 + -0.5 84,048 + +0.0 84,049 - -0.0 84,049 - +4.1 84,049 - +0.2 84,073 + +1.6 84,110 - +0.0 84,114 - -0.7 84,119 - +0.3 84,131 - +0.1 84,133 - -0.0 84,174 + +0.2 84,174 + -0.3 84,289 + +0.0 84,290 - +0.6 84,295 - +0.4 84,367 + GFF726 0.16 +0.2 84,367 + GFF726 0.16 -0.5 84,432 + GFF726 0.23 -0.1 84,433 - GFF726 0.23 -0.2 84,571 + GFF726 0.38 +0.0 84,572 - GFF726 0.38 +0.6 84,819 - GFF726 0.64 -0.2 84,819 - GFF726 0.64 -0.0
Or see this region's nucleotide sequence