Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4910 and GFF4911 are separated by 157 nucleotides GFF4911 and GFF4912 are separated by 38 nucleotides
GFF4910 - NADH oxidoreductase hcr (EC 1.-.-.-), at 12,122 to 13,093
GFF4910
GFF4911 - Pyruvate oxidase [ubiquinone, cytochrome] (EC 1.2.2.2), at 13,251 to 14,969
GFF4911
GFF4912 - Low-specificity L-threonine aldolase (EC 4.1.2.5), at 15,008 to 16,009
GFF4912
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1
2 at 12.328 kb on + strand, within GFF4910 at 12.328 kb on + strand, within GFF4910 at 12.329 kb on - strand, within GFF4910 at 12.329 kb on - strand, within GFF4910 at 12.329 kb on - strand, within GFF4910 at 12.329 kb on - strand, within GFF4910 at 12.404 kb on + strand, within GFF4910 at 12.741 kb on - strand, within GFF4910 at 12.919 kb on + strand, within GFF4910 at 12.919 kb on + strand, within GFF4910 at 12.920 kb on - strand, within GFF4910 at 13.043 kb on + strand at 13.048 kb on - strand at 13.234 kb on + strand at 13.358 kb on + strand at 13.553 kb on + strand, within GFF4911 at 13.554 kb on - strand, within GFF4911 at 13.700 kb on - strand, within GFF4911 at 14.186 kb on + strand, within GFF4911 at 14.627 kb on + strand, within GFF4911 at 15.029 kb on - strand at 15.094 kb on - strand at 15.094 kb on - strand at 15.235 kb on + strand, within GFF4912 at 15.256 kb on + strand, within GFF4912 at 15.260 kb on + strand, within GFF4912 at 15.260 kb on + strand, within GFF4912 at 15.261 kb on - strand, within GFF4912 at 15.261 kb on - strand, within GFF4912 at 15.449 kb on + strand, within GFF4912 at 15.450 kb on - strand, within GFF4912 at 15.473 kb on - strand, within GFF4912 at 15.473 kb on - strand, within GFF4912 at 15.549 kb on + strand, within GFF4912 at 15.550 kb on - strand, within GFF4912 at 15.567 kb on - strand, within GFF4912 at 15.671 kb on - strand, within GFF4912 at 15.725 kb on + strand, within GFF4912 at 15.726 kb on - strand, within GFF4912
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 12,328 + GFF4910 0.21 +0.2 12,328 + GFF4910 0.21 -0.0 12,329 - GFF4910 0.21 -2.2 12,329 - GFF4910 0.21 -0.0 12,329 - GFF4910 0.21 -0.4 12,329 - GFF4910 0.21 -0.4 12,404 + GFF4910 0.29 +0.2 12,741 - GFF4910 0.64 -0.1 12,919 + GFF4910 0.82 -1.6 12,919 + GFF4910 0.82 -0.2 12,920 - GFF4910 0.82 -0.2 13,043 + -0.3 13,048 - +0.3 13,234 + +0.3 13,358 + -0.4 13,553 + GFF4911 0.18 +0.2 13,554 - GFF4911 0.18 +0.1 13,700 - GFF4911 0.26 +1.4 14,186 + GFF4911 0.54 +0.0 14,627 + GFF4911 0.80 +0.8 15,029 - +0.9 15,094 - +0.3 15,094 - +0.4 15,235 + GFF4912 0.23 -0.0 15,256 + GFF4912 0.25 +0.5 15,260 + GFF4912 0.25 -0.2 15,260 + GFF4912 0.25 -0.6 15,261 - GFF4912 0.25 -0.7 15,261 - GFF4912 0.25 -0.2 15,449 + GFF4912 0.44 +0.1 15,450 - GFF4912 0.44 -0.5 15,473 - GFF4912 0.46 -0.4 15,473 - GFF4912 0.46 -0.3 15,549 + GFF4912 0.54 -0.8 15,550 - GFF4912 0.54 +0.2 15,567 - GFF4912 0.56 -0.3 15,671 - GFF4912 0.66 -0.2 15,725 + GFF4912 0.72 +0.9 15,726 - GFF4912 0.72 +1.8
Or see this region's nucleotide sequence