Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4838 and GFF4839 are separated by 57 nucleotides GFF4839 and GFF4840 are separated by 43 nucleotides
GFF4838 - 23S rRNA (Uracil-5-) -methyltransferase RumA (EC 2.1.1.-), at 135,132 to 136,427
GFF4838
GFF4839 - BarA sensory histidine kinase (= VarS = GacS), at 136,485 to 139,241
GFF4839
GFF4840 - Glycerate kinase (EC 2.7.1.31), at 139,285 to 140,427
GFF4840
Position (kb) 136
137
138
139
140 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 135.632 kb on + strand, within GFF4838 at 135.642 kb on - strand, within GFF4838 at 135.669 kb on + strand, within GFF4838 at 135.705 kb on + strand, within GFF4838 at 135.718 kb on - strand, within GFF4838 at 136.265 kb on + strand, within GFF4838 at 136.308 kb on - strand at 136.495 kb on - strand at 136.629 kb on - strand at 136.662 kb on - strand at 136.670 kb on - strand at 136.698 kb on - strand at 136.868 kb on + strand, within GFF4839 at 136.869 kb on - strand, within GFF4839 at 137.005 kb on - strand, within GFF4839 at 137.005 kb on - strand, within GFF4839 at 137.045 kb on + strand, within GFF4839 at 137.118 kb on - strand, within GFF4839 at 137.118 kb on - strand, within GFF4839 at 137.253 kb on - strand, within GFF4839 at 137.253 kb on - strand, within GFF4839 at 137.519 kb on + strand, within GFF4839 at 137.591 kb on + strand, within GFF4839 at 137.594 kb on + strand, within GFF4839 at 137.640 kb on - strand, within GFF4839 at 137.667 kb on - strand, within GFF4839 at 137.726 kb on + strand, within GFF4839 at 137.726 kb on + strand, within GFF4839 at 137.727 kb on - strand, within GFF4839 at 137.727 kb on - strand, within GFF4839 at 137.748 kb on - strand, within GFF4839 at 137.748 kb on - strand, within GFF4839 at 137.906 kb on + strand, within GFF4839 at 138.106 kb on - strand, within GFF4839 at 138.137 kb on + strand, within GFF4839 at 138.137 kb on + strand, within GFF4839 at 138.138 kb on - strand, within GFF4839 at 138.178 kb on - strand, within GFF4839 at 138.218 kb on + strand, within GFF4839 at 138.386 kb on - strand, within GFF4839 at 138.430 kb on - strand, within GFF4839 at 138.463 kb on + strand, within GFF4839 at 138.541 kb on + strand, within GFF4839 at 138.565 kb on + strand, within GFF4839 at 138.783 kb on + strand, within GFF4839 at 138.783 kb on + strand, within GFF4839 at 138.854 kb on - strand, within GFF4839 at 139.072 kb on + strand at 139.092 kb on - strand at 139.231 kb on + strand at 139.231 kb on + strand at 139.392 kb on - strand at 139.482 kb on + strand, within GFF4840 at 139.512 kb on + strand, within GFF4840 at 139.567 kb on + strand, within GFF4840 at 139.655 kb on + strand, within GFF4840 at 139.656 kb on - strand, within GFF4840 at 139.864 kb on + strand, within GFF4840 at 139.864 kb on + strand, within GFF4840 at 139.865 kb on - strand, within GFF4840 at 139.866 kb on + strand, within GFF4840 at 139.867 kb on - strand, within GFF4840 at 140.038 kb on - strand, within GFF4840 at 140.043 kb on + strand, within GFF4840 at 140.199 kb on - strand, within GFF4840
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 135,632 + GFF4838 0.39 -0.9 135,642 - GFF4838 0.39 -0.2 135,669 + GFF4838 0.41 +0.6 135,705 + GFF4838 0.44 +0.6 135,718 - GFF4838 0.45 +1.2 136,265 + GFF4838 0.87 -0.5 136,308 - +0.9 136,495 - -1.9 136,629 - -1.9 136,662 - -4.4 136,670 - -2.8 136,698 - -2.4 136,868 + GFF4839 0.14 -2.4 136,869 - GFF4839 0.14 -3.1 137,005 - GFF4839 0.19 -4.6 137,005 - GFF4839 0.19 -2.8 137,045 + GFF4839 0.20 -2.1 137,118 - GFF4839 0.23 -4.9 137,118 - GFF4839 0.23 -4.1 137,253 - GFF4839 0.28 -3.3 137,253 - GFF4839 0.28 -3.6 137,519 + GFF4839 0.38 -3.7 137,591 + GFF4839 0.40 -3.2 137,594 + GFF4839 0.40 -4.9 137,640 - GFF4839 0.42 -2.9 137,667 - GFF4839 0.43 -4.6 137,726 + GFF4839 0.45 -4.1 137,726 + GFF4839 0.45 -3.6 137,727 - GFF4839 0.45 -3.6 137,727 - GFF4839 0.45 -2.4 137,748 - GFF4839 0.46 -3.4 137,748 - GFF4839 0.46 -4.8 137,906 + GFF4839 0.52 -4.3 138,106 - GFF4839 0.59 -2.4 138,137 + GFF4839 0.60 -2.0 138,137 + GFF4839 0.60 -2.2 138,138 - GFF4839 0.60 -3.2 138,178 - GFF4839 0.61 -2.2 138,218 + GFF4839 0.63 -2.5 138,386 - GFF4839 0.69 -1.8 138,430 - GFF4839 0.71 -2.2 138,463 + GFF4839 0.72 -2.5 138,541 + GFF4839 0.75 -3.7 138,565 + GFF4839 0.75 -3.1 138,783 + GFF4839 0.83 -1.6 138,783 + GFF4839 0.83 -4.4 138,854 - GFF4839 0.86 -0.9 139,072 + -2.9 139,092 - -4.4 139,231 + +0.1 139,231 + -0.1 139,392 - +0.1 139,482 + GFF4840 0.17 -0.2 139,512 + GFF4840 0.20 -0.3 139,567 + GFF4840 0.25 +0.2 139,655 + GFF4840 0.32 -0.1 139,656 - GFF4840 0.32 +1.1 139,864 + GFF4840 0.51 -0.0 139,864 + GFF4840 0.51 +1.0 139,865 - GFF4840 0.51 -0.3 139,866 + GFF4840 0.51 -0.0 139,867 - GFF4840 0.51 +0.4 140,038 - GFF4840 0.66 -2.2 140,043 + GFF4840 0.66 +0.3 140,199 - GFF4840 0.80 -0.3
Or see this region's nucleotide sequence