Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4835 and GFF4836 overlap by 29 nucleotides GFF4836 and GFF4837 are separated by 152 nucleotides GFF4837 and GFF4838 are separated by 51 nucleotides
GFF4835 - ParE toxin protein, at 132,144 to 132,434
GFF4835
GFF4836 - Transcriptional regulator, ArsR family, at 132,406 to 132,693
GFF4836
GFF4837 - 'GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)' transl_table=11, at 132,846 to 135,080
GFF4837
GFF4838 - 23S rRNA (Uracil-5-) -methyltransferase RumA (EC 2.1.1.-), at 135,132 to 136,427
GFF4838
Position (kb) 132
133
134
135
136 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 131.913 kb on + strand at 131.955 kb on + strand at 132.191 kb on + strand, within GFF4835 at 132.191 kb on + strand, within GFF4835 at 132.330 kb on + strand, within GFF4835 at 132.708 kb on + strand at 132.769 kb on + strand at 132.791 kb on + strand at 132.791 kb on + strand at 132.826 kb on - strand at 132.947 kb on + strand at 133.122 kb on + strand, within GFF4837 at 133.386 kb on - strand, within GFF4837 at 133.637 kb on - strand, within GFF4837 at 134.118 kb on - strand, within GFF4837 at 134.152 kb on + strand, within GFF4837 at 134.190 kb on - strand, within GFF4837 at 134.309 kb on + strand, within GFF4837 at 134.310 kb on - strand, within GFF4837 at 134.312 kb on - strand, within GFF4837 at 134.312 kb on - strand, within GFF4837 at 134.312 kb on - strand, within GFF4837 at 134.315 kb on + strand, within GFF4837 at 134.399 kb on - strand, within GFF4837 at 134.479 kb on + strand, within GFF4837 at 134.537 kb on - strand, within GFF4837 at 134.664 kb on + strand, within GFF4837 at 134.665 kb on - strand, within GFF4837 at 134.800 kb on - strand, within GFF4837 at 134.814 kb on + strand, within GFF4837 at 134.815 kb on - strand, within GFF4837 at 134.871 kb on - strand at 134.871 kb on - strand at 135.051 kb on - strand at 135.054 kb on - strand at 135.056 kb on + strand at 135.056 kb on + strand at 135.057 kb on - strand at 135.057 kb on - strand at 135.119 kb on - strand at 135.133 kb on + strand at 135.133 kb on + strand at 135.134 kb on - strand at 135.275 kb on - strand, within GFF4838 at 135.300 kb on + strand, within GFF4838 at 135.632 kb on + strand, within GFF4838 at 135.642 kb on - strand, within GFF4838 at 135.669 kb on + strand, within GFF4838 at 135.705 kb on + strand, within GFF4838 at 135.718 kb on - strand, within GFF4838
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 131,913 + -0.3 131,955 + -0.6 132,191 + GFF4835 0.16 +0.3 132,191 + GFF4835 0.16 -0.2 132,330 + GFF4835 0.64 +1.5 132,708 + -0.2 132,769 + +0.5 132,791 + +0.5 132,791 + -0.5 132,826 - +0.1 132,947 + -2.0 133,122 + GFF4837 0.12 -1.8 133,386 - GFF4837 0.24 -2.0 133,637 - GFF4837 0.35 -1.0 134,118 - GFF4837 0.57 -2.2 134,152 + GFF4837 0.58 -2.1 134,190 - GFF4837 0.60 -2.3 134,309 + GFF4837 0.65 -1.6 134,310 - GFF4837 0.66 -3.8 134,312 - GFF4837 0.66 -1.8 134,312 - GFF4837 0.66 -2.7 134,312 - GFF4837 0.66 -2.5 134,315 + GFF4837 0.66 -1.0 134,399 - GFF4837 0.69 -1.3 134,479 + GFF4837 0.73 -2.5 134,537 - GFF4837 0.76 -3.8 134,664 + GFF4837 0.81 -1.7 134,665 - GFF4837 0.81 -2.8 134,800 - GFF4837 0.87 -2.6 134,814 + GFF4837 0.88 -2.8 134,815 - GFF4837 0.88 -3.4 134,871 - -1.0 134,871 - -1.9 135,051 - -3.5 135,054 - -3.8 135,056 + -4.7 135,056 + -0.5 135,057 - -3.5 135,057 - -0.8 135,119 - -1.8 135,133 + -2.2 135,133 + -2.0 135,134 - -1.0 135,275 - GFF4838 0.11 +0.2 135,300 + GFF4838 0.13 -1.4 135,632 + GFF4838 0.39 -0.9 135,642 - GFF4838 0.39 -0.2 135,669 + GFF4838 0.41 +0.6 135,705 + GFF4838 0.44 +0.6 135,718 - GFF4838 0.45 +1.2
Or see this region's nucleotide sequence