Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4723 and GFF4724 overlap by 4 nucleotides GFF4724 and GFF4725 are separated by 233 nucleotides
GFF4723 - [NiFe] hydrogenase metallocenter assembly protein HypD, at 31,138 to 32,259
GFF4723
GFF4724 - [NiFe] hydrogenase metallocenter assembly protein HypE, at 32,256 to 33,266
GFF4724
GFF4725 - Formate hydrogenlyase transcriptional activator, at 33,500 to 35,563
GFF4725
Position (kb)
32
33
34 Strain fitness (log2 ratio)
-1
0
1 at 31.305 kb on - strand, within GFF4723 at 31.497 kb on + strand, within GFF4723 at 31.691 kb on + strand, within GFF4723 at 31.833 kb on - strand, within GFF4723 at 31.833 kb on - strand, within GFF4723 at 31.897 kb on + strand, within GFF4723 at 32.083 kb on + strand, within GFF4723 at 32.084 kb on - strand, within GFF4723 at 32.084 kb on - strand, within GFF4723 at 32.266 kb on + strand at 32.267 kb on - strand at 32.267 kb on - strand at 32.324 kb on + strand at 32.433 kb on + strand, within GFF4724 at 32.433 kb on + strand, within GFF4724 at 32.434 kb on - strand, within GFF4724 at 32.597 kb on + strand, within GFF4724 at 32.683 kb on + strand, within GFF4724 at 32.688 kb on - strand, within GFF4724 at 32.777 kb on + strand, within GFF4724 at 32.864 kb on - strand, within GFF4724 at 32.892 kb on - strand, within GFF4724 at 33.207 kb on + strand at 33.250 kb on + strand at 33.257 kb on + strand at 33.259 kb on + strand at 33.265 kb on - strand at 33.283 kb on + strand at 33.283 kb on + strand at 33.297 kb on + strand at 33.412 kb on + strand at 33.424 kb on + strand at 33.425 kb on - strand at 33.493 kb on + strand at 33.493 kb on + strand at 33.542 kb on - strand at 33.542 kb on - strand at 33.546 kb on + strand at 33.597 kb on + strand at 33.598 kb on - strand at 33.666 kb on - strand at 33.666 kb on - strand at 33.701 kb on + strand at 33.843 kb on - strand, within GFF4725 at 33.946 kb on + strand, within GFF4725 at 33.946 kb on + strand, within GFF4725 at 33.947 kb on - strand, within GFF4725 at 34.013 kb on + strand, within GFF4725 at 34.105 kb on - strand, within GFF4725
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 31,305 - GFF4723 0.15 +0.0 31,497 + GFF4723 0.32 -0.3 31,691 + GFF4723 0.49 -0.5 31,833 - GFF4723 0.62 +0.8 31,833 - GFF4723 0.62 +0.9 31,897 + GFF4723 0.68 +0.2 32,083 + GFF4723 0.84 -1.0 32,084 - GFF4723 0.84 -0.0 32,084 - GFF4723 0.84 -0.0 32,266 + +0.6 32,267 - +0.6 32,267 - -0.2 32,324 + +0.2 32,433 + GFF4724 0.18 +0.3 32,433 + GFF4724 0.18 -0.0 32,434 - GFF4724 0.18 +0.1 32,597 + GFF4724 0.34 +0.1 32,683 + GFF4724 0.42 +0.2 32,688 - GFF4724 0.43 +1.4 32,777 + GFF4724 0.52 -0.8 32,864 - GFF4724 0.60 +0.4 32,892 - GFF4724 0.63 +0.0 33,207 + -0.2 33,250 + -0.3 33,257 + +0.6 33,259 + -0.2 33,265 - -0.6 33,283 + +0.5 33,283 + -0.2 33,297 + +0.9 33,412 + +0.5 33,424 + -0.1 33,425 - +0.2 33,493 + -0.1 33,493 + -1.1 33,542 - -0.5 33,542 - -0.0 33,546 + -0.2 33,597 + -0.1 33,598 - -0.4 33,666 - +1.6 33,666 - -0.0 33,701 + -0.4 33,843 - GFF4725 0.17 -0.4 33,946 + GFF4725 0.22 -0.0 33,946 + GFF4725 0.22 -0.0 33,947 - GFF4725 0.22 -0.1 34,013 + GFF4725 0.25 -0.0 34,105 - GFF4725 0.29 +0.4
Or see this region's nucleotide sequence