Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4586 and GFF4587 are separated by 326 nucleotides GFF4587 and GFF4588 are separated by 210 nucleotides
GFF4586 - Glucosamine-6-phosphate deaminase (EC 3.5.99.6), at 374,675 to 375,475
GFF4586
GFF4587 - PTS system, N-acetylglucosamine-specific IIA component (EC 2.7.1.69) / PTS system, N-acetylglucosamine-specific IIB component (EC 2.7.1.69) / PTS system, N-acetylglucosamine-specific IIC component (EC 2.7.1.69), at 375,802 to 377,754
GFF4587
GFF4588 - Glutaminyl-tRNA synthetase (EC 6.1.1.18), at 377,965 to 379,632
GFF4588
Position (kb)
375
376
377
378 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 374.980 kb on + strand, within GFF4586 at 374.981 kb on - strand, within GFF4586 at 375.266 kb on - strand, within GFF4586 at 375.403 kb on + strand at 375.404 kb on - strand at 375.404 kb on - strand at 375.407 kb on - strand at 375.524 kb on + strand at 375.657 kb on + strand at 375.735 kb on + strand at 375.798 kb on + strand at 375.808 kb on - strand at 375.912 kb on + strand at 375.913 kb on - strand at 375.913 kb on - strand at 375.997 kb on - strand at 376.110 kb on + strand, within GFF4587 at 376.199 kb on + strand, within GFF4587 at 376.199 kb on + strand, within GFF4587 at 376.266 kb on - strand, within GFF4587 at 376.326 kb on + strand, within GFF4587 at 376.377 kb on - strand, within GFF4587 at 376.434 kb on - strand, within GFF4587 at 376.434 kb on - strand, within GFF4587 at 376.460 kb on - strand, within GFF4587 at 376.546 kb on + strand, within GFF4587 at 376.653 kb on + strand, within GFF4587 at 376.771 kb on + strand, within GFF4587 at 376.806 kb on + strand, within GFF4587 at 376.839 kb on - strand, within GFF4587 at 376.852 kb on + strand, within GFF4587 at 376.881 kb on + strand, within GFF4587 at 376.882 kb on - strand, within GFF4587 at 376.987 kb on + strand, within GFF4587 at 376.988 kb on - strand, within GFF4587 at 376.988 kb on - strand, within GFF4587 at 376.988 kb on - strand, within GFF4587 at 377.037 kb on + strand, within GFF4587 at 377.038 kb on - strand, within GFF4587 at 377.040 kb on + strand, within GFF4587 at 377.041 kb on - strand, within GFF4587 at 377.439 kb on + strand, within GFF4587 at 377.511 kb on + strand, within GFF4587 at 377.645 kb on + strand at 377.646 kb on - strand at 377.722 kb on - strand at 377.735 kb on - strand at 377.917 kb on - strand at 377.918 kb on + strand at 377.918 kb on + strand at 377.926 kb on + strand at 377.936 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 374,980 + GFF4586 0.38 -0.9 374,981 - GFF4586 0.38 -1.1 375,266 - GFF4586 0.74 -1.4 375,403 + -0.6 375,404 - +0.2 375,404 - -2.1 375,407 - -0.9 375,524 + -0.0 375,657 + +0.4 375,735 + +0.8 375,798 + +0.0 375,808 - -0.0 375,912 + +0.4 375,913 - +1.2 375,913 - +0.8 375,997 - +0.2 376,110 + GFF4587 0.16 +0.0 376,199 + GFF4587 0.20 +1.8 376,199 + GFF4587 0.20 -0.2 376,266 - GFF4587 0.24 +1.2 376,326 + GFF4587 0.27 +0.5 376,377 - GFF4587 0.29 -1.9 376,434 - GFF4587 0.32 +0.7 376,434 - GFF4587 0.32 -0.1 376,460 - GFF4587 0.34 -0.1 376,546 + GFF4587 0.38 +0.7 376,653 + GFF4587 0.44 +0.1 376,771 + GFF4587 0.50 -0.3 376,806 + GFF4587 0.51 -0.3 376,839 - GFF4587 0.53 -0.3 376,852 + GFF4587 0.54 +0.0 376,881 + GFF4587 0.55 +0.6 376,882 - GFF4587 0.55 +0.3 376,987 + GFF4587 0.61 +0.1 376,988 - GFF4587 0.61 -0.3 376,988 - GFF4587 0.61 -0.3 376,988 - GFF4587 0.61 +0.3 377,037 + GFF4587 0.63 +1.2 377,038 - GFF4587 0.63 +0.3 377,040 + GFF4587 0.63 +0.4 377,041 - GFF4587 0.63 -0.8 377,439 + GFF4587 0.84 +0.3 377,511 + GFF4587 0.88 -0.6 377,645 + +0.2 377,646 - -0.6 377,722 - -0.3 377,735 - +0.4 377,917 - +0.3 377,918 + +0.8 377,918 + +0.3 377,926 + +1.0 377,936 + +0.2
Or see this region's nucleotide sequence