Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4585 and GFF4586 are separated by 59 nucleotides GFF4586 and GFF4587 are separated by 326 nucleotides
GFF4585 - N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25), at 373,461 to 374,615
GFF4585
GFF4586 - Glucosamine-6-phosphate deaminase (EC 3.5.99.6), at 374,675 to 375,475
GFF4586
GFF4587 - PTS system, N-acetylglucosamine-specific IIA component (EC 2.7.1.69) / PTS system, N-acetylglucosamine-specific IIB component (EC 2.7.1.69) / PTS system, N-acetylglucosamine-specific IIC component (EC 2.7.1.69), at 375,802 to 377,754
GFF4587
Position (kb) 374
375
376 Strain fitness (log2 ratio) -2
-1
0
1
2 at 373.704 kb on - strand, within GFF4585 at 373.892 kb on - strand, within GFF4585 at 373.892 kb on - strand, within GFF4585 at 373.948 kb on + strand, within GFF4585 at 374.319 kb on - strand, within GFF4585 at 374.337 kb on - strand, within GFF4585 at 374.339 kb on + strand, within GFF4585 at 374.340 kb on - strand, within GFF4585 at 374.340 kb on - strand, within GFF4585 at 374.340 kb on - strand, within GFF4585 at 374.406 kb on - strand, within GFF4585 at 374.612 kb on - strand at 374.980 kb on + strand, within GFF4586 at 374.981 kb on - strand, within GFF4586 at 375.266 kb on - strand, within GFF4586 at 375.403 kb on + strand at 375.404 kb on - strand at 375.404 kb on - strand at 375.407 kb on - strand at 375.524 kb on + strand at 375.657 kb on + strand at 375.735 kb on + strand at 375.798 kb on + strand at 375.808 kb on - strand at 375.912 kb on + strand at 375.913 kb on - strand at 375.913 kb on - strand at 375.997 kb on - strand at 376.110 kb on + strand, within GFF4587 at 376.199 kb on + strand, within GFF4587 at 376.199 kb on + strand, within GFF4587 at 376.266 kb on - strand, within GFF4587 at 376.326 kb on + strand, within GFF4587 at 376.377 kb on - strand, within GFF4587 at 376.434 kb on - strand, within GFF4587 at 376.434 kb on - strand, within GFF4587 at 376.460 kb on - strand, within GFF4587
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 373,704 - GFF4585 0.21 +0.3 373,892 - GFF4585 0.37 +0.7 373,892 - GFF4585 0.37 +1.0 373,948 + GFF4585 0.42 +0.3 374,319 - GFF4585 0.74 +0.4 374,337 - GFF4585 0.76 +0.8 374,339 + GFF4585 0.76 +0.5 374,340 - GFF4585 0.76 +0.4 374,340 - GFF4585 0.76 +0.0 374,340 - GFF4585 0.76 +0.5 374,406 - GFF4585 0.82 +0.9 374,612 - +0.6 374,980 + GFF4586 0.38 -0.9 374,981 - GFF4586 0.38 -1.1 375,266 - GFF4586 0.74 -1.4 375,403 + -0.6 375,404 - +0.2 375,404 - -2.1 375,407 - -0.9 375,524 + -0.0 375,657 + +0.4 375,735 + +0.8 375,798 + +0.0 375,808 - -0.0 375,912 + +0.4 375,913 - +1.2 375,913 - +0.8 375,997 - +0.2 376,110 + GFF4587 0.16 +0.0 376,199 + GFF4587 0.20 +1.8 376,199 + GFF4587 0.20 -0.2 376,266 - GFF4587 0.24 +1.2 376,326 + GFF4587 0.27 +0.5 376,377 - GFF4587 0.29 -1.9 376,434 - GFF4587 0.32 +0.7 376,434 - GFF4587 0.32 -0.1 376,460 - GFF4587 0.34 -0.1
Or see this region's nucleotide sequence