Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4577 and GFF4578 are separated by 58 nucleotides GFF4578 and GFF4579 are separated by 26 nucleotides GFF4579 and GFF4580 are separated by 143 nucleotides
GFF4577 - Phosphate starvation-inducible protein PhoH, predicted ATPase, at 363,450 to 364,496
GFF4577
GFF4578 - hypothetical protein, at 364,555 to 364,674
GFF4578
GFF4579 - tRNA-i(6)A37 methylthiotransferase, at 364,701 to 366,125
GFF4579
GFF4580 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (EC 1.14.13.-), at 366,269 to 367,444
GFF4580
Position (kb) 364
365
366
367 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 363.796 kb on - strand, within GFF4577 at 363.796 kb on - strand, within GFF4577 at 363.991 kb on - strand, within GFF4577 at 364.298 kb on - strand, within GFF4577 at 364.489 kb on - strand at 364.845 kb on - strand, within GFF4579 at 364.865 kb on + strand, within GFF4579 at 364.865 kb on + strand, within GFF4579 at 364.988 kb on + strand, within GFF4579 at 365.021 kb on + strand, within GFF4579 at 365.117 kb on + strand, within GFF4579 at 365.117 kb on + strand, within GFF4579 at 365.118 kb on - strand, within GFF4579 at 365.153 kb on + strand, within GFF4579 at 365.270 kb on - strand, within GFF4579 at 365.372 kb on - strand, within GFF4579 at 365.374 kb on - strand, within GFF4579 at 365.381 kb on + strand, within GFF4579 at 365.384 kb on + strand, within GFF4579 at 365.478 kb on - strand, within GFF4579 at 365.638 kb on + strand, within GFF4579 at 365.639 kb on - strand, within GFF4579 at 365.642 kb on + strand, within GFF4579 at 365.651 kb on + strand, within GFF4579 at 365.651 kb on + strand, within GFF4579 at 365.823 kb on - strand, within GFF4579 at 365.920 kb on - strand, within GFF4579 at 365.985 kb on - strand at 365.985 kb on - strand at 365.985 kb on - strand at 366.031 kb on + strand at 366.105 kb on - strand at 366.108 kb on - strand at 366.167 kb on + strand at 366.168 kb on - strand at 366.172 kb on + strand at 366.199 kb on - strand at 366.199 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 363,796 - GFF4577 0.33 +0.4 363,796 - GFF4577 0.33 +0.1 363,991 - GFF4577 0.52 -0.2 364,298 - GFF4577 0.81 -0.3 364,489 - +0.4 364,845 - GFF4579 0.10 -1.6 364,865 + GFF4579 0.12 -1.1 364,865 + GFF4579 0.12 -1.6 364,988 + GFF4579 0.20 -1.1 365,021 + GFF4579 0.22 -0.7 365,117 + GFF4579 0.29 -1.4 365,117 + GFF4579 0.29 -1.4 365,118 - GFF4579 0.29 +0.5 365,153 + GFF4579 0.32 -1.0 365,270 - GFF4579 0.40 -1.2 365,372 - GFF4579 0.47 -1.1 365,374 - GFF4579 0.47 -1.7 365,381 + GFF4579 0.48 -2.0 365,384 + GFF4579 0.48 -2.1 365,478 - GFF4579 0.55 -2.0 365,638 + GFF4579 0.66 -0.7 365,639 - GFF4579 0.66 -1.0 365,642 + GFF4579 0.66 +0.6 365,651 + GFF4579 0.67 -1.1 365,651 + GFF4579 0.67 +0.2 365,823 - GFF4579 0.79 +0.4 365,920 - GFF4579 0.86 -1.8 365,985 - -3.1 365,985 - -1.2 365,985 - -1.8 366,031 + -0.8 366,105 - -1.3 366,108 - -1.4 366,167 + -2.2 366,168 - -0.5 366,172 + -1.1 366,199 - +0.3 366,199 - +0.6
Or see this region's nucleotide sequence