Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4291 and GFF4292 are separated by 21 nucleotides GFF4292 and GFF4293 are separated by 172 nucleotides
GFF4291 - Glucokinase, ROK family (EC 2.7.1.2), at 74,178 to 75,086
GFF4291
GFF4292 - Protein AraJ precursor, at 75,108 to 76,280
GFF4292
GFF4293 - Exonuclease SbcC, at 76,453 to 79,593
GFF4293
Position (kb) 75
76
77 Strain fitness (log2 ratio) -1
0
1 at 74.175 kb on + strand at 74.310 kb on - strand, within GFF4291 at 74.388 kb on + strand, within GFF4291 at 74.417 kb on + strand, within GFF4291 at 74.552 kb on - strand, within GFF4291 at 74.596 kb on + strand, within GFF4291 at 74.707 kb on - strand, within GFF4291 at 74.784 kb on - strand, within GFF4291 at 75.020 kb on - strand at 75.023 kb on + strand at 75.157 kb on + strand at 75.157 kb on + strand at 75.158 kb on - strand at 75.215 kb on - strand at 75.496 kb on + strand, within GFF4292 at 75.531 kb on + strand, within GFF4292 at 75.531 kb on + strand, within GFF4292 at 75.561 kb on - strand, within GFF4292 at 75.599 kb on - strand, within GFF4292 at 75.605 kb on + strand, within GFF4292 at 75.606 kb on - strand, within GFF4292 at 75.606 kb on - strand, within GFF4292 at 75.606 kb on - strand, within GFF4292 at 75.619 kb on + strand, within GFF4292 at 76.025 kb on + strand, within GFF4292 at 76.025 kb on + strand, within GFF4292 at 76.073 kb on + strand, within GFF4292 at 76.074 kb on - strand, within GFF4292 at 76.079 kb on + strand, within GFF4292 at 76.141 kb on + strand, within GFF4292 at 76.155 kb on - strand, within GFF4292 at 76.350 kb on - strand at 76.371 kb on + strand at 76.406 kb on - strand at 76.406 kb on - strand at 76.413 kb on + strand at 76.413 kb on + strand at 76.413 kb on + strand at 76.413 kb on + strand at 76.413 kb on + strand at 76.413 kb on + strand at 76.427 kb on - strand at 76.455 kb on - strand at 76.516 kb on + strand at 76.920 kb on + strand, within GFF4293 at 76.920 kb on + strand, within GFF4293 at 76.921 kb on - strand, within GFF4293 at 76.921 kb on - strand, within GFF4293 at 76.955 kb on + strand, within GFF4293 at 77.131 kb on - strand, within GFF4293
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 74,175 + +0.3 74,310 - GFF4291 0.15 -0.7 74,388 + GFF4291 0.23 +0.0 74,417 + GFF4291 0.26 +0.5 74,552 - GFF4291 0.41 -0.4 74,596 + GFF4291 0.46 -1.3 74,707 - GFF4291 0.58 +0.0 74,784 - GFF4291 0.67 -0.1 75,020 - +0.4 75,023 + +0.1 75,157 + -0.0 75,157 + -0.6 75,158 - +0.5 75,215 - +0.2 75,496 + GFF4292 0.33 -0.3 75,531 + GFF4292 0.36 -0.5 75,531 + GFF4292 0.36 -0.3 75,561 - GFF4292 0.39 -0.3 75,599 - GFF4292 0.42 +0.2 75,605 + GFF4292 0.42 +0.5 75,606 - GFF4292 0.42 -0.3 75,606 - GFF4292 0.42 +0.5 75,606 - GFF4292 0.42 -0.6 75,619 + GFF4292 0.44 -0.1 76,025 + GFF4292 0.78 +0.3 76,025 + GFF4292 0.78 +0.0 76,073 + GFF4292 0.82 -0.0 76,074 - GFF4292 0.82 +0.6 76,079 + GFF4292 0.83 -0.3 76,141 + GFF4292 0.88 -0.3 76,155 - GFF4292 0.89 -0.5 76,350 - +0.3 76,371 + -0.8 76,406 - -0.2 76,406 - +0.4 76,413 + +0.5 76,413 + +0.3 76,413 + -1.0 76,413 + +0.2 76,413 + +1.6 76,413 + +0.1 76,427 - -0.2 76,455 - -0.1 76,516 + +0.2 76,920 + GFF4293 0.15 +0.1 76,920 + GFF4293 0.15 +0.3 76,921 - GFF4293 0.15 +0.3 76,921 - GFF4293 0.15 -0.5 76,955 + GFF4293 0.16 +0.4 77,131 - GFF4293 0.22 +0.0
Or see this region's nucleotide sequence