Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF386

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF385 and GFF386 are separated by 22 nucleotidesGFF386 and GFF387 are separated by 149 nucleotides GFF385 - UDP-glucose:(glucosyl)lipopolysaccharide alpha-1,2-glucosyltransferase (EC 2.4.1.58), at 99,581 to 100,591 GFF385 GFF386 - Lipopolysaccharide core biosynthesis protein RfaY, at 100,614 to 101,312 GFF386 GFF387 - Lipopolysaccharide core biosynthesis protein RfaZ, at 101,462 to 102,271 GFF387 Position (kb) 100 101 102Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 100.418 kb on - strand, within GFF385at 100.954 kb on - strand, within GFF386at 100.958 kb on - strand, within GFF386at 101.056 kb on + strand, within GFF386at 101.507 kb on - strandat 101.982 kb on - strand, within GFF387at 102.025 kb on + strand, within GFF387at 102.028 kb on - strand, within GFF387

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
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100,418 - GFF385 0.83 +1.0
100,954 - GFF386 0.49 -1.5
100,958 - GFF386 0.49 -3.2
101,056 + GFF386 0.63 -0.9
101,507 - +2.3
101,982 - GFF387 0.64 +2.5
102,025 + GFF387 0.70 -0.4
102,028 - GFF387 0.70 +2.4

Or see this region's nucleotide sequence