Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3700 and GFF3701 are separated by 11 nucleotides GFF3701 and GFF3702 are separated by 109 nucleotides GFF3702 and GFF3703 are separated by 0 nucleotides GFF3703 and GFF3704 overlap by 4 nucleotides
GFF3700 - Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 6,100 to 7,473
GFF3700
GFF3701 - Nitrite transporter NirC, at 7,485 to 8,294
GFF3701
GFF3702 - hypothetical protein, at 8,404 to 8,559
GFF3702
GFF3703 - Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4), at 8,560 to 8,886
GFF3703
GFF3704 - Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4), at 8,883 to 11,390
GFF3704
Position (kb)
7
8
9 Strain fitness (log2 ratio)
-1
0
1
2
3 at 6.709 kb on - strand, within GFF3700 at 7.140 kb on + strand, within GFF3700 at 7.141 kb on - strand, within GFF3700 at 7.321 kb on + strand, within GFF3700 at 7.486 kb on + strand at 7.596 kb on - strand, within GFF3701 at 7.644 kb on - strand, within GFF3701 at 7.645 kb on + strand, within GFF3701 at 7.646 kb on - strand, within GFF3701 at 7.749 kb on - strand, within GFF3701 at 7.927 kb on + strand, within GFF3701 at 7.985 kb on + strand, within GFF3701 at 8.127 kb on + strand, within GFF3701 at 8.185 kb on + strand, within GFF3701 at 8.186 kb on - strand, within GFF3701 at 8.186 kb on - strand, within GFF3701 at 8.273 kb on + strand at 8.366 kb on + strand at 8.366 kb on + strand at 8.374 kb on + strand at 8.374 kb on + strand at 8.375 kb on - strand at 8.375 kb on - strand at 8.501 kb on + strand, within GFF3702 at 8.590 kb on + strand at 8.638 kb on + strand, within GFF3703 at 8.786 kb on + strand, within GFF3703 at 8.787 kb on - strand, within GFF3703 at 8.923 kb on + strand at 9.208 kb on + strand, within GFF3704 at 9.209 kb on - strand, within GFF3704 at 9.218 kb on + strand, within GFF3704 at 9.233 kb on - strand, within GFF3704
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 6,709 - GFF3700 0.44 +0.5 7,140 + GFF3700 0.76 +3.7 7,141 - GFF3700 0.76 +0.7 7,321 + GFF3700 0.89 +1.9 7,486 + -0.0 7,596 - GFF3701 0.14 +0.3 7,644 - GFF3701 0.20 +0.1 7,645 + GFF3701 0.20 +0.0 7,646 - GFF3701 0.20 +0.1 7,749 - GFF3701 0.33 -0.5 7,927 + GFF3701 0.55 +0.5 7,985 + GFF3701 0.62 +0.2 8,127 + GFF3701 0.79 +0.5 8,185 + GFF3701 0.86 +0.2 8,186 - GFF3701 0.87 +0.3 8,186 - GFF3701 0.87 -0.3 8,273 + -0.2 8,366 + -0.6 8,366 + -0.5 8,374 + +0.1 8,374 + +0.1 8,375 - +0.3 8,375 - -0.1 8,501 + GFF3702 0.62 -0.0 8,590 + +0.7 8,638 + GFF3703 0.24 -0.0 8,786 + GFF3703 0.69 -0.4 8,787 - GFF3703 0.69 -0.3 8,923 + +0.2 9,208 + GFF3704 0.13 +0.4 9,209 - GFF3704 0.13 +0.2 9,218 + GFF3704 0.13 -0.1 9,233 - GFF3704 0.14 +0.0
Or see this region's nucleotide sequence