Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3483 and GFF3484 are separated by 173 nucleotides GFF3484 and GFF3485 are separated by 117 nucleotides GFF3485 and GFF3486 are separated by 4 nucleotides
GFF3483 - Signal peptide peptidase SppA (EC 3.4.21.-), at 234,963 to 236,819
GFF3483
GFF3484 - FIG002003: Protein YdjA, at 236,993 to 237,544
GFF3484
GFF3485 - Selenide,water dikinase (EC 2.7.9.3), at 237,662 to 238,705
GFF3485
GFF3486 - DNA topoisomerase III (EC 5.99.1.2), at 238,710 to 240,659
GFF3486
Position (kb)
237
238
239 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 236.821 kb on + strand at 236.821 kb on + strand at 236.822 kb on - strand at 236.851 kb on - strand at 236.873 kb on - strand at 236.967 kb on + strand at 237.070 kb on - strand, within GFF3484 at 237.262 kb on + strand, within GFF3484 at 237.602 kb on + strand at 237.603 kb on - strand at 237.603 kb on - strand at 237.634 kb on - strand at 237.746 kb on - strand at 237.995 kb on - strand, within GFF3485 at 238.084 kb on + strand, within GFF3485 at 238.085 kb on - strand, within GFF3485 at 238.129 kb on - strand, within GFF3485 at 238.334 kb on - strand, within GFF3485 at 238.451 kb on + strand, within GFF3485 at 238.451 kb on + strand, within GFF3485 at 238.452 kb on - strand, within GFF3485 at 238.452 kb on - strand, within GFF3485 at 238.454 kb on - strand, within GFF3485 at 238.502 kb on + strand, within GFF3485 at 238.832 kb on + strand at 238.974 kb on - strand, within GFF3486 at 239.061 kb on + strand, within GFF3486 at 239.144 kb on + strand, within GFF3486 at 239.231 kb on + strand, within GFF3486 at 239.232 kb on - strand, within GFF3486 at 239.418 kb on - strand, within GFF3486 at 239.544 kb on + strand, within GFF3486 at 239.665 kb on + strand, within GFF3486 at 239.666 kb on - strand, within GFF3486 at 239.667 kb on + strand, within GFF3486 at 239.667 kb on + strand, within GFF3486
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 236,821 + +0.2 236,821 + +0.6 236,822 - +0.0 236,851 - -0.4 236,873 - +0.8 236,967 + -0.2 237,070 - GFF3484 0.14 -0.1 237,262 + GFF3484 0.49 +0.3 237,602 + +0.3 237,603 - +0.7 237,603 - -0.0 237,634 - -0.0 237,746 - +0.1 237,995 - GFF3485 0.32 +0.9 238,084 + GFF3485 0.40 -0.1 238,085 - GFF3485 0.41 +0.5 238,129 - GFF3485 0.45 +0.5 238,334 - GFF3485 0.64 +0.4 238,451 + GFF3485 0.76 +0.0 238,451 + GFF3485 0.76 +0.0 238,452 - GFF3485 0.76 +2.6 238,452 - GFF3485 0.76 +0.4 238,454 - GFF3485 0.76 +0.7 238,502 + GFF3485 0.80 +0.3 238,832 + +0.4 238,974 - GFF3486 0.14 -2.2 239,061 + GFF3486 0.18 +0.0 239,144 + GFF3486 0.22 -0.1 239,231 + GFF3486 0.27 -0.6 239,232 - GFF3486 0.27 +0.7 239,418 - GFF3486 0.36 +0.4 239,544 + GFF3486 0.43 -0.2 239,665 + GFF3486 0.49 +0.0 239,666 - GFF3486 0.49 +0.1 239,667 + GFF3486 0.49 -0.2 239,667 + GFF3486 0.49 +0.2
Or see this region's nucleotide sequence