Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3349 and GFF3350 are separated by 81 nucleotides GFF3350 and GFF3351 are separated by 34 nucleotides GFF3351 and GFF3352 are separated by 0 nucleotides GFF3352 and GFF3353 overlap by 26 nucleotides GFF3353 and GFF3354 are separated by 156 nucleotides GFF3354 and GFF3355 are separated by 3 nucleotides GFF3355 and GFF3356 are separated by 11 nucleotides
GFF3349 - Proposed peptidoglycan lipid II flippase MurJ, at 117,015 to 118,589
GFF3349
GFF3350 - Flagellar biosynthesis protein FlgN, at 118,671 to 119,063
GFF3350
GFF3351 - Negative regulator of flagellin synthesis FlgM, at 119,098 to 119,391
GFF3351
GFF3352 - hypothetical protein, at 119,392 to 119,508
GFF3352
GFF3353 - Flagellar basal-body P-ring formation protein FlgA, at 119,483 to 120,142
GFF3353
GFF3354 - Flagellar basal-body rod protein FlgB, at 120,299 to 120,715
GFF3354
GFF3355 - Flagellar basal-body rod protein FlgC, at 120,719 to 121,123
GFF3355
GFF3356 - Flagellar basal-body rod modification protein FlgD, at 121,135 to 121,833
GFF3356
Position (kb)
119
120
121 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 118.624 kb on - strand at 118.729 kb on + strand, within GFF3350 at 118.729 kb on + strand, within GFF3350 at 118.967 kb on + strand, within GFF3350 at 118.971 kb on - strand, within GFF3350 at 118.977 kb on + strand, within GFF3350 at 119.094 kb on - strand at 119.100 kb on - strand at 119.100 kb on - strand at 119.104 kb on - strand at 119.104 kb on - strand at 119.115 kb on - strand at 119.215 kb on - strand, within GFF3351 at 119.582 kb on - strand, within GFF3353 at 119.639 kb on + strand, within GFF3353 at 119.675 kb on + strand, within GFF3353 at 119.676 kb on - strand, within GFF3353 at 119.683 kb on + strand, within GFF3353 at 120.146 kb on - strand at 120.179 kb on - strand at 120.207 kb on - strand at 120.210 kb on + strand at 120.211 kb on - strand at 120.261 kb on + strand at 120.262 kb on - strand at 120.298 kb on + strand at 120.401 kb on - strand, within GFF3354 at 120.589 kb on + strand, within GFF3354 at 120.593 kb on - strand, within GFF3354 at 120.598 kb on - strand, within GFF3354 at 120.598 kb on - strand, within GFF3354 at 120.601 kb on + strand, within GFF3354 at 120.602 kb on - strand, within GFF3354 at 120.950 kb on + strand, within GFF3355 at 120.986 kb on + strand, within GFF3355 at 120.987 kb on - strand, within GFF3355 at 121.052 kb on + strand, within GFF3355 at 121.053 kb on - strand, within GFF3355 at 121.064 kb on - strand, within GFF3355 at 121.135 kb on - strand at 121.140 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 118,624 - +0.1 118,729 + GFF3350 0.15 -1.9 118,729 + GFF3350 0.15 -0.2 118,967 + GFF3350 0.75 +0.2 118,971 - GFF3350 0.76 +0.8 118,977 + GFF3350 0.78 +0.3 119,094 - +0.3 119,100 - +0.1 119,100 - +0.3 119,104 - +0.3 119,104 - +0.3 119,115 - +0.1 119,215 - GFF3351 0.40 -1.0 119,582 - GFF3353 0.15 +1.0 119,639 + GFF3353 0.24 +0.9 119,675 + GFF3353 0.29 +1.1 119,676 - GFF3353 0.29 +1.4 119,683 + GFF3353 0.30 +0.8 120,146 - +0.1 120,179 - -1.0 120,207 - +0.1 120,210 + +0.4 120,211 - +1.3 120,261 + +1.5 120,262 - +1.3 120,298 + +1.9 120,401 - GFF3354 0.24 +0.6 120,589 + GFF3354 0.70 +1.2 120,593 - GFF3354 0.71 +1.0 120,598 - GFF3354 0.72 +0.3 120,598 - GFF3354 0.72 +0.9 120,601 + GFF3354 0.72 +1.4 120,602 - GFF3354 0.73 +0.8 120,950 + GFF3355 0.57 -0.6 120,986 + GFF3355 0.66 +0.9 120,987 - GFF3355 0.66 +1.9 121,052 + GFF3355 0.82 +1.5 121,053 - GFF3355 0.82 +0.9 121,064 - GFF3355 0.85 +1.4 121,135 - +0.8 121,140 + +0.7
Or see this region's nucleotide sequence