Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3335 and GFF3336 overlap by 4 nucleotides GFF3336 and GFF3337 are separated by 10 nucleotides GFF3337 and GFF3338 are separated by 48 nucleotides GFF3338 and GFF3339 are separated by 40 nucleotides GFF3339 and GFF3340 are separated by 112 nucleotides GFF3340 and GFF3341 are separated by 286 nucleotides GFF3341 and GFF3342 are separated by 73 nucleotides
GFF3335 - Protein yceI precursor, at 106,911 to 107,486
GFF3335
GFF3336 - Cytochrome B561, at 107,483 to 108,055
GFF3336
GFF3337 - FIG01045781: hypothetical protein, at 108,066 to 108,269
GFF3337
GFF3338 - hypothetical protein, at 108,318 to 108,431
GFF3338
GFF3339 - N-methyl-L-amino-acid oxidase (EC 1.5.3.2); N-methyl-L-tryptophan oxidase (EC 1.5.3.-), at 108,472 to 109,590
GFF3339
GFF3340 - FIG074102: hypothetical protein, at 109,703 to 109,960
GFF3340
GFF3341 - DNA-damage-inducible protein I, at 110,247 to 110,492
GFF3341
GFF3342 - Dihydroorotase (EC 3.5.2.3), at 110,566 to 111,612
GFF3342
Position (kb)
108
109
110 Strain fitness (log2 ratio)
-1
0
1 at 107.564 kb on + strand, within GFF3336 at 107.593 kb on + strand, within GFF3336 at 107.593 kb on + strand, within GFF3336 at 107.594 kb on - strand, within GFF3336 at 107.662 kb on + strand, within GFF3336 at 107.812 kb on - strand, within GFF3336 at 107.900 kb on - strand, within GFF3336 at 107.937 kb on + strand, within GFF3336 at 108.071 kb on + strand at 108.334 kb on + strand, within GFF3338 at 108.436 kb on + strand at 108.473 kb on + strand at 108.666 kb on - strand, within GFF3339 at 108.670 kb on - strand, within GFF3339 at 108.686 kb on - strand, within GFF3339 at 108.901 kb on - strand, within GFF3339 at 109.089 kb on + strand, within GFF3339 at 109.138 kb on - strand, within GFF3339 at 109.190 kb on + strand, within GFF3339 at 109.191 kb on - strand, within GFF3339 at 109.348 kb on - strand, within GFF3339 at 109.412 kb on + strand, within GFF3339 at 109.575 kb on + strand at 109.605 kb on + strand at 109.644 kb on + strand at 109.791 kb on - strand, within GFF3340 at 109.888 kb on - strand, within GFF3340 at 109.888 kb on - strand, within GFF3340 at 109.888 kb on - strand, within GFF3340 at 109.979 kb on - strand at 110.037 kb on - strand at 110.226 kb on + strand at 110.406 kb on - strand, within GFF3341 at 110.475 kb on - strand at 110.487 kb on - strand at 110.542 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 107,564 + GFF3336 0.14 +0.6 107,593 + GFF3336 0.19 +0.2 107,593 + GFF3336 0.19 +0.1 107,594 - GFF3336 0.19 +0.3 107,662 + GFF3336 0.31 +0.3 107,812 - GFF3336 0.57 +0.5 107,900 - GFF3336 0.73 -0.2 107,937 + GFF3336 0.79 +0.1 108,071 + +0.8 108,334 + GFF3338 0.14 +0.1 108,436 + +0.1 108,473 + -0.0 108,666 - GFF3339 0.17 -0.2 108,670 - GFF3339 0.18 +0.1 108,686 - GFF3339 0.19 -0.7 108,901 - GFF3339 0.38 +1.3 109,089 + GFF3339 0.55 +0.3 109,138 - GFF3339 0.60 -0.3 109,190 + GFF3339 0.64 +0.2 109,191 - GFF3339 0.64 -0.1 109,348 - GFF3339 0.78 +0.8 109,412 + GFF3339 0.84 +0.5 109,575 + +0.0 109,605 + -1.0 109,644 + +0.5 109,791 - GFF3340 0.34 +0.0 109,888 - GFF3340 0.72 -0.5 109,888 - GFF3340 0.72 -0.1 109,888 - GFF3340 0.72 -0.2 109,979 - +0.4 110,037 - -0.1 110,226 + -1.2 110,406 - GFF3341 0.65 -0.3 110,475 - -0.4 110,487 - -0.1 110,542 + -0.2
Or see this region's nucleotide sequence