Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3328 and GFF3329 overlap by 8 nucleotides GFF3329 and GFF3330 are separated by 97 nucleotides GFF3330 and GFF3331 are separated by 0 nucleotides GFF3331 and GFF3332 are separated by 81 nucleotides
GFF3328 - Glucans biosynthesis protein G precursor, at 98,451 to 100,004
GFF3328
GFF3329 - Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-), at 99,997 to 102,540
GFF3329
GFF3330 - Putative outer membrane lipoprotein, at 102,638 to 102,841
GFF3330
GFF3331 - Acidic protein msyB, at 102,842 to 103,216
GFF3331
GFF3332 - Multidrug-efflux transporter, major facilitator superfamily (MFS) (TC 2.A.1), at 103,298 to 104,512
GFF3332
Position (kb) 99
100
101
102
103 Strain fitness (log2 ratio) -1
0
1
2 at 99.224 kb on + strand, within GFF3328 at 99.298 kb on + strand, within GFF3328 at 99.372 kb on + strand, within GFF3328 at 99.373 kb on - strand, within GFF3328 at 99.453 kb on + strand, within GFF3328 at 99.551 kb on + strand, within GFF3328 at 99.848 kb on + strand, within GFF3328 at 99.853 kb on + strand at 99.853 kb on + strand at 99.854 kb on - strand at 100.115 kb on - strand at 100.200 kb on - strand at 100.200 kb on - strand at 100.403 kb on + strand, within GFF3329 at 100.404 kb on - strand, within GFF3329 at 100.404 kb on - strand, within GFF3329 at 100.404 kb on - strand, within GFF3329 at 100.459 kb on + strand, within GFF3329 at 100.460 kb on - strand, within GFF3329 at 100.461 kb on + strand, within GFF3329 at 100.462 kb on - strand, within GFF3329 at 100.506 kb on + strand, within GFF3329 at 100.507 kb on - strand, within GFF3329 at 100.527 kb on + strand, within GFF3329 at 100.690 kb on - strand, within GFF3329 at 100.837 kb on + strand, within GFF3329 at 100.838 kb on - strand, within GFF3329 at 100.856 kb on - strand, within GFF3329 at 101.017 kb on + strand, within GFF3329 at 101.017 kb on + strand, within GFF3329 at 101.018 kb on - strand, within GFF3329 at 101.018 kb on - strand, within GFF3329 at 101.019 kb on + strand, within GFF3329 at 101.020 kb on - strand, within GFF3329 at 101.142 kb on + strand, within GFF3329 at 101.580 kb on + strand, within GFF3329 at 101.638 kb on + strand, within GFF3329 at 101.752 kb on - strand, within GFF3329 at 101.754 kb on + strand, within GFF3329 at 101.769 kb on + strand, within GFF3329 at 101.769 kb on + strand, within GFF3329 at 102.310 kb on - strand at 102.482 kb on - strand at 102.482 kb on - strand at 102.539 kb on - strand at 102.620 kb on - strand at 102.620 kb on - strand at 102.637 kb on - strand at 102.839 kb on + strand at 102.844 kb on - strand at 102.938 kb on - strand, within GFF3331 at 103.094 kb on + strand, within GFF3331 at 103.095 kb on - strand, within GFF3331 at 103.095 kb on - strand, within GFF3331 at 103.095 kb on - strand, within GFF3331 at 103.099 kb on - strand, within GFF3331 at 103.206 kb on + strand at 103.207 kb on - strand at 103.207 kb on - strand at 103.207 kb on - strand at 103.244 kb on - strand at 103.244 kb on - strand at 103.258 kb on + strand at 103.468 kb on - strand, within GFF3332 at 103.476 kb on - strand, within GFF3332
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 99,224 + GFF3328 0.50 +1.5 99,298 + GFF3328 0.55 +0.8 99,372 + GFF3328 0.59 +1.4 99,373 - GFF3328 0.59 +1.0 99,453 + GFF3328 0.64 +1.2 99,551 + GFF3328 0.71 +1.5 99,848 + GFF3328 0.90 +1.2 99,853 + +1.2 99,853 + +0.9 99,854 - +1.0 100,115 - +1.3 100,200 - -1.2 100,200 - +1.2 100,403 + GFF3329 0.16 +1.4 100,404 - GFF3329 0.16 +1.4 100,404 - GFF3329 0.16 +1.1 100,404 - GFF3329 0.16 +1.1 100,459 + GFF3329 0.18 +1.2 100,460 - GFF3329 0.18 +1.3 100,461 + GFF3329 0.18 +0.8 100,462 - GFF3329 0.18 +0.9 100,506 + GFF3329 0.20 +0.7 100,507 - GFF3329 0.20 +2.0 100,527 + GFF3329 0.21 +1.8 100,690 - GFF3329 0.27 +1.0 100,837 + GFF3329 0.33 +1.0 100,838 - GFF3329 0.33 +1.1 100,856 - GFF3329 0.34 +1.0 101,017 + GFF3329 0.40 +1.6 101,017 + GFF3329 0.40 +1.8 101,018 - GFF3329 0.40 +1.5 101,018 - GFF3329 0.40 +1.2 101,019 + GFF3329 0.40 +1.3 101,020 - GFF3329 0.40 +0.7 101,142 + GFF3329 0.45 +2.2 101,580 + GFF3329 0.62 +1.2 101,638 + GFF3329 0.65 +1.2 101,752 - GFF3329 0.69 +2.3 101,754 + GFF3329 0.69 +1.8 101,769 + GFF3329 0.70 +1.5 101,769 + GFF3329 0.70 +1.2 102,310 - +1.0 102,482 - +1.0 102,482 - +1.4 102,539 - +0.5 102,620 - -0.5 102,620 - -0.1 102,637 - -0.0 102,839 + +0.0 102,844 - -0.0 102,938 - GFF3331 0.26 -0.2 103,094 + GFF3331 0.67 -0.2 103,095 - GFF3331 0.67 +0.2 103,095 - GFF3331 0.67 +1.2 103,095 - GFF3331 0.67 -0.7 103,099 - GFF3331 0.69 +0.0 103,206 + +0.4 103,207 - +0.0 103,207 - +0.2 103,207 - +1.0 103,244 - +0.5 103,244 - +0.4 103,258 + -0.2 103,468 - GFF3332 0.14 +0.3 103,476 - GFF3332 0.15 +0.2
Or see this region's nucleotide sequence