Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3284 and GFF3285 are separated by 9 nucleotides GFF3285 and GFF3286 are separated by 13 nucleotides
GFF3284 - 4-hydroxyphenylacetate symporter, major facilitator superfamily (MFS), at 55,902 to 57,278
GFF3284
GFF3285 - Transcriptional activator of 4-hydroxyphenylacetate 3-monooxygenase operon, XylS/AraC family, at 57,288 to 58,184
GFF3285
GFF3286 - Ribonuclease Z (EC 3.1.26.11), at 58,198 to 59,136
GFF3286
Position (kb) 57
58
59 Strain fitness (log2 ratio) -1
0
1 at 56.332 kb on + strand, within GFF3284 at 56.444 kb on + strand, within GFF3284 at 56.445 kb on - strand, within GFF3284 at 56.445 kb on - strand, within GFF3284 at 56.465 kb on - strand, within GFF3284 at 56.531 kb on + strand, within GFF3284 at 56.713 kb on + strand, within GFF3284 at 56.714 kb on - strand, within GFF3284 at 56.721 kb on + strand, within GFF3284 at 56.722 kb on - strand, within GFF3284 at 56.722 kb on - strand, within GFF3284 at 56.723 kb on + strand, within GFF3284 at 56.750 kb on + strand, within GFF3284 at 56.768 kb on + strand, within GFF3284 at 57.287 kb on + strand at 57.287 kb on + strand at 57.287 kb on + strand at 57.287 kb on + strand at 57.288 kb on - strand at 57.317 kb on + strand at 57.545 kb on + strand, within GFF3285 at 57.791 kb on + strand, within GFF3285 at 57.881 kb on - strand, within GFF3285 at 57.881 kb on - strand, within GFF3285 at 58.218 kb on + strand at 58.392 kb on - strand, within GFF3286 at 58.459 kb on - strand, within GFF3286 at 58.491 kb on - strand, within GFF3286 at 58.545 kb on + strand, within GFF3286 at 58.546 kb on - strand, within GFF3286 at 58.550 kb on + strand, within GFF3286 at 58.699 kb on + strand, within GFF3286 at 58.699 kb on + strand, within GFF3286 at 58.701 kb on + strand, within GFF3286 at 58.818 kb on + strand, within GFF3286 at 58.918 kb on - strand, within GFF3286 at 59.022 kb on - strand, within GFF3286 at 59.115 kb on + strand at 59.115 kb on + strand at 59.123 kb on + strand at 59.123 kb on + strand at 59.134 kb on + strand at 59.134 kb on + strand at 59.135 kb on - strand at 59.135 kb on - strand at 59.139 kb on - strand at 59.139 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 56,332 + GFF3284 0.31 +0.2 56,444 + GFF3284 0.39 -0.3 56,445 - GFF3284 0.39 +0.1 56,445 - GFF3284 0.39 -0.8 56,465 - GFF3284 0.41 +0.3 56,531 + GFF3284 0.46 +0.1 56,713 + GFF3284 0.59 +0.0 56,714 - GFF3284 0.59 -0.5 56,721 + GFF3284 0.59 +0.5 56,722 - GFF3284 0.60 +0.4 56,722 - GFF3284 0.60 +0.2 56,723 + GFF3284 0.60 +0.4 56,750 + GFF3284 0.62 -0.3 56,768 + GFF3284 0.63 +0.3 57,287 + -0.6 57,287 + -1.1 57,287 + +0.6 57,287 + +0.0 57,288 - +0.3 57,317 + +0.7 57,545 + GFF3285 0.29 -0.9 57,791 + GFF3285 0.56 -1.1 57,881 - GFF3285 0.66 -0.8 57,881 - GFF3285 0.66 -0.1 58,218 + +0.4 58,392 - GFF3286 0.21 -0.9 58,459 - GFF3286 0.28 -0.4 58,491 - GFF3286 0.31 -0.2 58,545 + GFF3286 0.37 -0.8 58,546 - GFF3286 0.37 -0.6 58,550 + GFF3286 0.37 -0.3 58,699 + GFF3286 0.53 -0.0 58,699 + GFF3286 0.53 -0.7 58,701 + GFF3286 0.54 -0.5 58,818 + GFF3286 0.66 +0.4 58,918 - GFF3286 0.77 +0.0 59,022 - GFF3286 0.88 +0.1 59,115 + +0.6 59,115 + +0.3 59,123 + +0.0 59,123 + -0.1 59,134 + +0.0 59,134 + -1.4 59,135 - +0.5 59,135 - +0.4 59,139 - +0.6 59,139 - -0.7
Or see this region's nucleotide sequence