Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3190 and GFF3191 are separated by 254 nucleotides GFF3191 and GFF3192 are separated by 449 nucleotides
GFF3190 - Integral membrane protein TerC, at 197,412 to 198,380
GFF3190
GFF3191 - Sodium:dicarboxylate symporter, at 198,635 to 199,879
GFF3191
GFF3192 - DedA family inner membrane protein YqjA, at 200,329 to 200,991
GFF3192
Position (kb) 198
199
200 Strain fitness (log2 ratio) -1
0
1
2 at 197.714 kb on + strand, within GFF3190 at 197.715 kb on - strand, within GFF3190 at 197.844 kb on + strand, within GFF3190 at 197.996 kb on + strand, within GFF3190 at 198.012 kb on + strand, within GFF3190 at 198.012 kb on + strand, within GFF3190 at 198.081 kb on - strand, within GFF3190 at 198.081 kb on - strand, within GFF3190 at 198.122 kb on - strand, within GFF3190 at 198.148 kb on + strand, within GFF3190 at 198.211 kb on - strand, within GFF3190 at 198.267 kb on + strand, within GFF3190 at 198.268 kb on - strand, within GFF3190 at 198.299 kb on - strand at 198.541 kb on + strand at 198.543 kb on + strand at 198.568 kb on - strand at 198.620 kb on + strand at 198.661 kb on - strand at 198.831 kb on + strand, within GFF3191 at 199.012 kb on - strand, within GFF3191 at 199.012 kb on - strand, within GFF3191 at 199.075 kb on + strand, within GFF3191 at 199.076 kb on - strand, within GFF3191 at 199.079 kb on + strand, within GFF3191 at 199.080 kb on - strand, within GFF3191 at 199.080 kb on - strand, within GFF3191 at 199.080 kb on - strand, within GFF3191 at 199.133 kb on - strand, within GFF3191 at 199.309 kb on + strand, within GFF3191 at 199.444 kb on + strand, within GFF3191 at 199.492 kb on + strand, within GFF3191 at 199.492 kb on + strand, within GFF3191 at 199.497 kb on - strand, within GFF3191 at 199.526 kb on - strand, within GFF3191 at 199.532 kb on - strand, within GFF3191 at 199.583 kb on - strand, within GFF3191 at 199.740 kb on + strand, within GFF3191 at 199.874 kb on - strand at 199.874 kb on - strand at 199.878 kb on - strand at 199.878 kb on - strand at 199.903 kb on + strand at 199.904 kb on - strand at 199.924 kb on - strand at 199.924 kb on - strand at 199.924 kb on - strand at 199.924 kb on - strand at 199.946 kb on - strand at 199.993 kb on + strand at 199.999 kb on + strand at 199.999 kb on + strand at 199.999 kb on + strand at 200.000 kb on - strand at 200.000 kb on - strand at 200.000 kb on - strand at 200.002 kb on - strand at 200.005 kb on + strand at 200.005 kb on + strand at 200.042 kb on + strand at 200.191 kb on + strand at 200.192 kb on - strand at 200.323 kb on + strand at 200.323 kb on + strand at 200.337 kb on - strand at 200.412 kb on - strand, within GFF3192 at 200.414 kb on - strand, within GFF3192 at 200.424 kb on - strand, within GFF3192 at 200.656 kb on + strand, within GFF3192 at 200.657 kb on - strand, within GFF3192 at 200.872 kb on - strand, within GFF3192 at 200.878 kb on + strand, within GFF3192 at 200.878 kb on + strand, within GFF3192
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 197,714 + GFF3190 0.31 +0.7 197,715 - GFF3190 0.31 +0.1 197,844 + GFF3190 0.45 +0.1 197,996 + GFF3190 0.60 -0.2 198,012 + GFF3190 0.62 +0.2 198,012 + GFF3190 0.62 +0.4 198,081 - GFF3190 0.69 -0.6 198,081 - GFF3190 0.69 -0.5 198,122 - GFF3190 0.73 +1.5 198,148 + GFF3190 0.76 -0.2 198,211 - GFF3190 0.82 +0.1 198,267 + GFF3190 0.88 -0.0 198,268 - GFF3190 0.88 +0.1 198,299 - +0.0 198,541 + -1.1 198,543 + +0.1 198,568 - -0.1 198,620 + +0.6 198,661 - +0.1 198,831 + GFF3191 0.16 +0.9 199,012 - GFF3191 0.30 -0.1 199,012 - GFF3191 0.30 -0.1 199,075 + GFF3191 0.35 -0.2 199,076 - GFF3191 0.35 +1.4 199,079 + GFF3191 0.36 -0.0 199,080 - GFF3191 0.36 -0.2 199,080 - GFF3191 0.36 -0.0 199,080 - GFF3191 0.36 -0.2 199,133 - GFF3191 0.40 -0.3 199,309 + GFF3191 0.54 +0.0 199,444 + GFF3191 0.65 -0.1 199,492 + GFF3191 0.69 -0.1 199,492 + GFF3191 0.69 +0.6 199,497 - GFF3191 0.69 +0.2 199,526 - GFF3191 0.72 -0.4 199,532 - GFF3191 0.72 -0.4 199,583 - GFF3191 0.76 -0.1 199,740 + GFF3191 0.89 +0.3 199,874 - -0.2 199,874 - -0.1 199,878 - -0.6 199,878 - +0.3 199,903 + -0.4 199,904 - +2.1 199,924 - -0.1 199,924 - -0.7 199,924 - -0.0 199,924 - +0.1 199,946 - +0.0 199,993 + +1.1 199,999 + -0.1 199,999 + -0.7 199,999 + +0.2 200,000 - -0.2 200,000 - +0.0 200,000 - +0.1 200,002 - -0.6 200,005 + -0.1 200,005 + -0.7 200,042 + -0.4 200,191 + +0.2 200,192 - -0.8 200,323 + -1.2 200,323 + +0.3 200,337 - +0.5 200,412 - GFF3192 0.13 -0.3 200,414 - GFF3192 0.13 -0.6 200,424 - GFF3192 0.14 -0.5 200,656 + GFF3192 0.49 -0.9 200,657 - GFF3192 0.49 -0.4 200,872 - GFF3192 0.82 -0.9 200,878 + GFF3192 0.83 -0.6 200,878 + GFF3192 0.83 -0.6
Or see this region's nucleotide sequence