Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2890 and GFF2891 are separated by 418 nucleotides GFF2891 and GFF2892 are separated by 143 nucleotides GFF2892 and GFF2893 are separated by 118 nucleotides
GFF2890 - Transcription termination factor Rho, at 14,347 to 15,606
GFF2890
GFF2891 - Thioredoxin, at 16,025 to 16,354
GFF2891
GFF2892 - ATP-dependent RNA helicase RhlB, at 16,498 to 17,763
GFF2892
GFF2893 - 'Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (EC 3.6.1.40) @ Exopolyphosphatase (EC 3.6.1.11)' transl_table=11, at 17,882 to 19,363
GFF2893
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 15.647 kb on - strand at 15.955 kb on - strand at 15.955 kb on - strand at 16.016 kb on + strand at 16.017 kb on - strand at 16.024 kb on - strand at 16.024 kb on - strand at 16.108 kb on + strand, within GFF2891 at 16.143 kb on + strand, within GFF2891 at 16.157 kb on - strand, within GFF2891 at 16.304 kb on + strand, within GFF2891 at 16.304 kb on + strand, within GFF2891 at 16.354 kb on + strand at 16.355 kb on - strand at 16.356 kb on + strand at 16.357 kb on - strand at 16.357 kb on - strand at 16.357 kb on - strand at 16.358 kb on + strand at 16.358 kb on + strand at 16.358 kb on + strand at 16.372 kb on + strand at 16.373 kb on - strand at 16.391 kb on + strand at 16.392 kb on - strand at 16.418 kb on + strand at 16.443 kb on + strand at 16.484 kb on + strand at 16.490 kb on - strand at 16.497 kb on + strand at 16.705 kb on + strand, within GFF2892 at 16.790 kb on + strand, within GFF2892 at 16.912 kb on - strand, within GFF2892 at 16.917 kb on + strand, within GFF2892 at 17.022 kb on + strand, within GFF2892 at 17.052 kb on + strand, within GFF2892 at 17.207 kb on - strand, within GFF2892 at 17.234 kb on + strand, within GFF2892 at 17.234 kb on + strand, within GFF2892 at 17.310 kb on + strand, within GFF2892 at 17.484 kb on + strand, within GFF2892 at 17.484 kb on + strand, within GFF2892 at 17.489 kb on - strand, within GFF2892 at 17.516 kb on - strand, within GFF2892 at 17.587 kb on + strand, within GFF2892 at 17.617 kb on + strand, within GFF2892 at 17.772 kb on - strand at 17.792 kb on + strand at 17.792 kb on + strand at 17.799 kb on + strand at 17.800 kb on - strand at 17.834 kb on + strand at 17.834 kb on + strand at 17.843 kb on + strand at 17.844 kb on - strand at 17.882 kb on - strand at 17.901 kb on - strand at 17.986 kb on - strand at 18.184 kb on + strand, within GFF2893 at 18.208 kb on + strand, within GFF2893 at 18.208 kb on + strand, within GFF2893 at 18.404 kb on - strand, within GFF2893 at 18.571 kb on + strand, within GFF2893 at 18.595 kb on - strand, within GFF2893 at 18.595 kb on - strand, within GFF2893
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 15,647 - -2.3 15,955 - +0.1 15,955 - +0.4 16,016 + +0.5 16,017 - +0.0 16,024 - +0.6 16,024 - +0.2 16,108 + GFF2891 0.25 -0.1 16,143 + GFF2891 0.36 -0.4 16,157 - GFF2891 0.40 +0.0 16,304 + GFF2891 0.85 -0.4 16,304 + GFF2891 0.85 -0.4 16,354 + -0.1 16,355 - +0.4 16,356 + -0.1 16,357 - +0.2 16,357 - -0.3 16,357 - +0.1 16,358 + -0.3 16,358 + -0.4 16,358 + +0.4 16,372 + -1.3 16,373 - +0.0 16,391 + +0.2 16,392 - +0.4 16,418 + +0.1 16,443 + +0.0 16,484 + +0.5 16,490 - +1.0 16,497 + +0.4 16,705 + GFF2892 0.16 +1.8 16,790 + GFF2892 0.23 +1.4 16,912 - GFF2892 0.33 +1.2 16,917 + GFF2892 0.33 +1.9 17,022 + GFF2892 0.41 -0.7 17,052 + GFF2892 0.44 +0.3 17,207 - GFF2892 0.56 +1.2 17,234 + GFF2892 0.58 +1.0 17,234 + GFF2892 0.58 +1.6 17,310 + GFF2892 0.64 +1.3 17,484 + GFF2892 0.78 +1.1 17,484 + GFF2892 0.78 +2.0 17,489 - GFF2892 0.78 +1.0 17,516 - GFF2892 0.80 +0.9 17,587 + GFF2892 0.86 +0.6 17,617 + GFF2892 0.88 +0.7 17,772 - +1.1 17,792 + +0.9 17,792 + +0.3 17,799 + +0.8 17,800 - +0.7 17,834 + +0.3 17,834 + +0.4 17,843 + +0.7 17,844 - +1.1 17,882 - +0.9 17,901 - +1.3 17,986 - +0.7 18,184 + GFF2893 0.20 +1.4 18,208 + GFF2893 0.22 +0.9 18,208 + GFF2893 0.22 +0.6 18,404 - GFF2893 0.35 +0.9 18,571 + GFF2893 0.46 +1.0 18,595 - GFF2893 0.48 +1.1 18,595 - GFF2893 0.48 +1.2
Or see this region's nucleotide sequence