Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2888 and GFF2889 are separated by 11 nucleotides GFF2889 and GFF2890 are separated by 229 nucleotides
GFF2888 - regulator of length of O-antigen component of lipopolysaccharide chains, at 11,956 to 13,002
GFF2888
GFF2889 - Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-), at 13,014 to 14,117
GFF2889
GFF2890 - Transcription termination factor Rho, at 14,347 to 15,606
GFF2890
Position (kb) 13
14
15 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 12.076 kb on - strand, within GFF2888 at 12.132 kb on + strand, within GFF2888 at 12.132 kb on + strand, within GFF2888 at 12.132 kb on + strand, within GFF2888 at 12.216 kb on + strand, within GFF2888 at 12.230 kb on - strand, within GFF2888 at 12.230 kb on - strand, within GFF2888 at 12.288 kb on + strand, within GFF2888 at 12.288 kb on + strand, within GFF2888 at 12.291 kb on + strand, within GFF2888 at 12.325 kb on - strand, within GFF2888 at 12.470 kb on + strand, within GFF2888 at 12.470 kb on + strand, within GFF2888 at 12.566 kb on - strand, within GFF2888 at 12.566 kb on - strand, within GFF2888 at 12.633 kb on - strand, within GFF2888 at 12.636 kb on - strand, within GFF2888 at 12.636 kb on - strand, within GFF2888 at 12.701 kb on + strand, within GFF2888 at 12.701 kb on + strand, within GFF2888 at 12.751 kb on - strand, within GFF2888 at 12.751 kb on - strand, within GFF2888 at 12.754 kb on - strand, within GFF2888 at 12.786 kb on - strand, within GFF2888 at 12.801 kb on + strand, within GFF2888 at 12.801 kb on + strand, within GFF2888 at 12.802 kb on - strand, within GFF2888 at 12.802 kb on - strand, within GFF2888 at 12.802 kb on - strand, within GFF2888 at 12.805 kb on + strand, within GFF2888 at 12.806 kb on - strand, within GFF2888 at 12.862 kb on - strand, within GFF2888 at 13.015 kb on + strand at 13.023 kb on + strand at 13.280 kb on + strand, within GFF2889 at 13.280 kb on + strand, within GFF2889 at 13.280 kb on + strand, within GFF2889 at 13.280 kb on + strand, within GFF2889 at 13.330 kb on + strand, within GFF2889 at 13.395 kb on - strand, within GFF2889 at 13.457 kb on + strand, within GFF2889 at 13.457 kb on + strand, within GFF2889 at 13.458 kb on - strand, within GFF2889 at 13.458 kb on - strand, within GFF2889 at 13.458 kb on - strand, within GFF2889 at 13.458 kb on - strand, within GFF2889 at 13.487 kb on - strand, within GFF2889 at 13.517 kb on + strand, within GFF2889 at 13.523 kb on - strand, within GFF2889 at 13.587 kb on + strand, within GFF2889 at 13.647 kb on - strand, within GFF2889 at 13.698 kb on - strand, within GFF2889 at 13.726 kb on - strand, within GFF2889 at 13.788 kb on + strand, within GFF2889 at 13.886 kb on + strand, within GFF2889 at 13.891 kb on + strand, within GFF2889 at 13.893 kb on + strand, within GFF2889 at 13.894 kb on - strand, within GFF2889 at 13.900 kb on - strand, within GFF2889 at 13.908 kb on + strand, within GFF2889 at 13.909 kb on - strand, within GFF2889 at 13.912 kb on + strand, within GFF2889 at 13.915 kb on + strand, within GFF2889 at 13.998 kb on - strand, within GFF2889 at 14.122 kb on + strand at 14.123 kb on - strand at 14.156 kb on - strand at 14.199 kb on + strand at 14.225 kb on - strand at 14.270 kb on + strand at 14.272 kb on + strand at 14.273 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 12,076 - GFF2888 0.11 +0.8 12,132 + GFF2888 0.17 +0.3 12,132 + GFF2888 0.17 -0.2 12,132 + GFF2888 0.17 +0.4 12,216 + GFF2888 0.25 +1.3 12,230 - GFF2888 0.26 -1.5 12,230 - GFF2888 0.26 +1.0 12,288 + GFF2888 0.32 +0.8 12,288 + GFF2888 0.32 +1.4 12,291 + GFF2888 0.32 +0.2 12,325 - GFF2888 0.35 +0.6 12,470 + GFF2888 0.49 +0.5 12,470 + GFF2888 0.49 +1.2 12,566 - GFF2888 0.58 +0.3 12,566 - GFF2888 0.58 +0.8 12,633 - GFF2888 0.65 +0.9 12,636 - GFF2888 0.65 +1.0 12,636 - GFF2888 0.65 +0.9 12,701 + GFF2888 0.71 +1.1 12,701 + GFF2888 0.71 +0.8 12,751 - GFF2888 0.76 +0.6 12,751 - GFF2888 0.76 +1.0 12,754 - GFF2888 0.76 +0.8 12,786 - GFF2888 0.79 +0.9 12,801 + GFF2888 0.81 +0.9 12,801 + GFF2888 0.81 +0.6 12,802 - GFF2888 0.81 +0.8 12,802 - GFF2888 0.81 +0.8 12,802 - GFF2888 0.81 +1.4 12,805 + GFF2888 0.81 +0.6 12,806 - GFF2888 0.81 +0.6 12,862 - GFF2888 0.87 +0.9 13,015 + +0.8 13,023 + -2.6 13,280 + GFF2889 0.24 -1.0 13,280 + GFF2889 0.24 -2.0 13,280 + GFF2889 0.24 -2.5 13,280 + GFF2889 0.24 -2.4 13,330 + GFF2889 0.29 -2.8 13,395 - GFF2889 0.35 -0.4 13,457 + GFF2889 0.40 -1.4 13,457 + GFF2889 0.40 -0.7 13,458 - GFF2889 0.40 -2.1 13,458 - GFF2889 0.40 -3.6 13,458 - GFF2889 0.40 -2.5 13,458 - GFF2889 0.40 -1.8 13,487 - GFF2889 0.43 -0.7 13,517 + GFF2889 0.46 -0.1 13,523 - GFF2889 0.46 -0.8 13,587 + GFF2889 0.52 -2.0 13,647 - GFF2889 0.57 -2.1 13,698 - GFF2889 0.62 -0.7 13,726 - GFF2889 0.64 -4.2 13,788 + GFF2889 0.70 -2.2 13,886 + GFF2889 0.79 -2.0 13,891 + GFF2889 0.79 -2.8 13,893 + GFF2889 0.80 -1.7 13,894 - GFF2889 0.80 -2.2 13,900 - GFF2889 0.80 -2.4 13,908 + GFF2889 0.81 -1.9 13,909 - GFF2889 0.81 -2.2 13,912 + GFF2889 0.81 +0.5 13,915 + GFF2889 0.82 -1.4 13,998 - GFF2889 0.89 -2.7 14,122 + -2.0 14,123 - -1.1 14,156 - -0.5 14,199 + -0.2 14,225 - +0.3 14,270 + +0.2 14,272 + +0.1 14,273 - +0.2
Or see this region's nucleotide sequence