Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2677 and GFF2678 are separated by 115 nucleotides GFF2678 and GFF2679 are separated by 89 nucleotides GFF2679 and GFF2680 are separated by 147 nucleotides
GFF2677 - L-alanine-DL-glutamate epimerase, at 200,121 to 201,086
GFF2677
GFF2678 - Thiol peroxidase, Tpx-type (EC 1.11.1.15), at 201,202 to 201,708
GFF2678
GFF2679 - Transcriptional repressor protein TyrR, at 201,798 to 203,339
GFF2679
GFF2680 - Membrane protein YcjF, at 203,487 to 204,548
GFF2680
Position (kb)
201
202
203
204 Strain fitness (log2 ratio)
-2
-1
0
1 at 200.803 kb on - strand, within GFF2677 at 200.952 kb on - strand, within GFF2677 at 201.045 kb on - strand at 201.069 kb on + strand at 201.069 kb on + strand at 201.070 kb on - strand at 201.070 kb on - strand at 201.075 kb on - strand at 201.130 kb on + strand at 201.179 kb on + strand at 201.181 kb on + strand at 201.197 kb on + strand at 201.285 kb on + strand, within GFF2678 at 201.286 kb on - strand, within GFF2678 at 201.514 kb on - strand, within GFF2678 at 201.532 kb on - strand, within GFF2678 at 201.634 kb on - strand, within GFF2678 at 201.707 kb on - strand at 201.743 kb on - strand at 201.901 kb on + strand at 201.906 kb on - strand at 201.948 kb on - strand at 202.244 kb on + strand, within GFF2679 at 202.386 kb on - strand, within GFF2679 at 202.516 kb on - strand, within GFF2679 at 202.644 kb on - strand, within GFF2679 at 202.722 kb on - strand, within GFF2679 at 202.722 kb on - strand, within GFF2679 at 202.722 kb on - strand, within GFF2679 at 202.957 kb on - strand, within GFF2679 at 203.048 kb on + strand, within GFF2679 at 203.268 kb on - strand at 203.272 kb on - strand at 203.281 kb on - strand at 203.369 kb on - strand at 203.369 kb on - strand at 203.369 kb on - strand at 203.420 kb on + strand at 203.507 kb on + strand at 203.574 kb on - strand at 203.574 kb on - strand at 203.605 kb on - strand, within GFF2680 at 203.791 kb on + strand, within GFF2680 at 203.791 kb on + strand, within GFF2680 at 203.794 kb on + strand, within GFF2680 at 203.812 kb on + strand, within GFF2680 at 203.812 kb on + strand, within GFF2680 at 203.812 kb on + strand, within GFF2680 at 203.868 kb on - strand, within GFF2680 at 204.025 kb on + strand, within GFF2680 at 204.117 kb on - strand, within GFF2680 at 204.120 kb on + strand, within GFF2680 at 204.289 kb on - strand, within GFF2680
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 200,803 - GFF2677 0.71 +0.6 200,952 - GFF2677 0.86 +0.1 201,045 - +0.1 201,069 + +0.2 201,069 + +0.0 201,070 - -0.2 201,070 - +0.2 201,075 - -0.8 201,130 + +0.2 201,179 + +0.1 201,181 + +0.1 201,197 + -0.4 201,285 + GFF2678 0.16 -0.3 201,286 - GFF2678 0.17 +0.6 201,514 - GFF2678 0.62 +0.2 201,532 - GFF2678 0.65 +0.4 201,634 - GFF2678 0.85 -0.9 201,707 - +0.2 201,743 - -0.4 201,901 + -2.3 201,906 - -1.7 201,948 - -0.6 202,244 + GFF2679 0.29 -1.4 202,386 - GFF2679 0.38 +1.2 202,516 - GFF2679 0.47 -0.8 202,644 - GFF2679 0.55 -1.3 202,722 - GFF2679 0.60 -1.2 202,722 - GFF2679 0.60 -1.3 202,722 - GFF2679 0.60 -0.4 202,957 - GFF2679 0.75 -0.5 203,048 + GFF2679 0.81 -1.1 203,268 - -0.7 203,272 - -0.2 203,281 - -0.7 203,369 - -0.4 203,369 - -0.2 203,369 - -1.0 203,420 + +0.2 203,507 + +0.6 203,574 - -0.3 203,574 - -0.3 203,605 - GFF2680 0.11 +0.4 203,791 + GFF2680 0.29 +0.0 203,791 + GFF2680 0.29 +0.3 203,794 + GFF2680 0.29 +0.1 203,812 + GFF2680 0.31 +0.3 203,812 + GFF2680 0.31 -0.1 203,812 + GFF2680 0.31 -1.2 203,868 - GFF2680 0.36 -0.1 204,025 + GFF2680 0.51 -0.9 204,117 - GFF2680 0.59 +0.3 204,120 + GFF2680 0.60 +0.4 204,289 - GFF2680 0.76 +0.2
Or see this region's nucleotide sequence