Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2610 and GFF2611 are separated by 11 nucleotides GFF2611 and GFF2612 are separated by 204 nucleotides GFF2612 and GFF2613 are separated by 255 nucleotides
GFF2610 - Lactate 2-monooxygenase (EC 1.13.12.4), at 133,460 to 134,662
GFF2610
GFF2611 - FIG01200701: possible membrane protein, at 134,674 to 135,240
GFF2611
GFF2612 - Glucans biosynthesis protein D precursor, at 135,445 to 137,064
GFF2612
GFF2613 - Putative esterase, at 137,320 to 138,828
GFF2613
Position (kb) 135
136
137
138 Strain fitness (log2 ratio) -1
0
1
2 at 134.595 kb on + strand at 134.706 kb on + strand at 135.279 kb on + strand at 135.507 kb on - strand at 135.507 kb on - strand at 135.552 kb on + strand at 135.552 kb on + strand at 135.552 kb on + strand at 135.552 kb on + strand at 135.553 kb on - strand at 135.553 kb on - strand at 135.606 kb on + strand at 136.035 kb on - strand, within GFF2612 at 136.056 kb on - strand, within GFF2612 at 136.113 kb on + strand, within GFF2612 at 136.113 kb on + strand, within GFF2612 at 136.115 kb on + strand, within GFF2612 at 136.115 kb on + strand, within GFF2612 at 136.116 kb on - strand, within GFF2612 at 136.246 kb on - strand, within GFF2612 at 136.258 kb on - strand, within GFF2612 at 136.311 kb on - strand, within GFF2612 at 136.419 kb on + strand, within GFF2612 at 136.490 kb on + strand, within GFF2612 at 136.491 kb on - strand, within GFF2612 at 136.494 kb on + strand, within GFF2612 at 136.495 kb on - strand, within GFF2612 at 136.499 kb on - strand, within GFF2612 at 136.532 kb on + strand, within GFF2612 at 136.533 kb on - strand, within GFF2612 at 136.537 kb on - strand, within GFF2612 at 136.537 kb on - strand, within GFF2612 at 136.609 kb on - strand, within GFF2612 at 136.609 kb on - strand, within GFF2612 at 136.735 kb on - strand, within GFF2612 at 136.858 kb on - strand, within GFF2612 at 137.083 kb on - strand at 137.101 kb on - strand at 137.101 kb on - strand at 137.331 kb on + strand at 137.570 kb on + strand, within GFF2613 at 137.601 kb on + strand, within GFF2613 at 137.612 kb on + strand, within GFF2613 at 137.656 kb on - strand, within GFF2613 at 137.675 kb on + strand, within GFF2613 at 137.676 kb on - strand, within GFF2613 at 137.735 kb on + strand, within GFF2613 at 137.749 kb on - strand, within GFF2613 at 137.752 kb on + strand, within GFF2613 at 137.752 kb on + strand, within GFF2613 at 137.752 kb on + strand, within GFF2613 at 137.753 kb on - strand, within GFF2613 at 137.753 kb on - strand, within GFF2613 at 137.753 kb on - strand, within GFF2613 at 137.780 kb on - strand, within GFF2613
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 134,595 + +0.5 134,706 + +0.3 135,279 + -0.0 135,507 - +0.9 135,507 - +1.0 135,552 + -0.1 135,552 + +1.1 135,552 + +0.7 135,552 + +0.9 135,553 - +0.5 135,553 - +1.0 135,606 + +1.9 136,035 - GFF2612 0.36 +1.3 136,056 - GFF2612 0.38 +0.7 136,113 + GFF2612 0.41 +1.1 136,113 + GFF2612 0.41 +0.2 136,115 + GFF2612 0.41 +0.0 136,115 + GFF2612 0.41 +1.1 136,116 - GFF2612 0.41 +0.9 136,246 - GFF2612 0.49 +1.1 136,258 - GFF2612 0.50 +0.9 136,311 - GFF2612 0.53 +0.7 136,419 + GFF2612 0.60 +1.0 136,490 + GFF2612 0.65 +0.9 136,491 - GFF2612 0.65 +0.6 136,494 + GFF2612 0.65 +1.6 136,495 - GFF2612 0.65 +0.1 136,499 - GFF2612 0.65 +0.5 136,532 + GFF2612 0.67 +1.0 136,533 - GFF2612 0.67 +1.0 136,537 - GFF2612 0.67 +1.0 136,537 - GFF2612 0.67 +0.4 136,609 - GFF2612 0.72 +0.9 136,609 - GFF2612 0.72 +0.2 136,735 - GFF2612 0.80 +1.3 136,858 - GFF2612 0.87 +1.6 137,083 - +0.8 137,101 - +1.7 137,101 - +1.0 137,331 + -0.5 137,570 + GFF2613 0.17 -0.5 137,601 + GFF2613 0.19 -0.6 137,612 + GFF2613 0.19 +0.3 137,656 - GFF2613 0.22 +1.1 137,675 + GFF2613 0.24 -0.2 137,676 - GFF2613 0.24 +0.9 137,735 + GFF2613 0.28 -0.5 137,749 - GFF2613 0.28 -1.2 137,752 + GFF2613 0.29 +1.1 137,752 + GFF2613 0.29 -0.1 137,752 + GFF2613 0.29 +0.1 137,753 - GFF2613 0.29 +0.1 137,753 - GFF2613 0.29 -0.5 137,753 - GFF2613 0.29 -0.2 137,780 - GFF2613 0.30 -0.0
Or see this region's nucleotide sequence