Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2585 and GFF2586 overlap by 4 nucleotides GFF2586 and GFF2587 are separated by 44 nucleotides GFF2587 and GFF2588 are separated by 398 nucleotides
GFF2585 - SrfB, at 106,713 to 109,694
GFF2585
GFF2586 - Putative virulence factor, at 109,691 to 111,835
GFF2586
GFF2587 - FIG00638290: hypothetical protein, at 111,880 to 112,053
GFF2587
GFF2588 - 4-aminobutyraldehyde dehydrogenase (EC 1.2.1.19), at 112,452 to 113,897
GFF2588
Position (kb) 109
110
111
112 Strain fitness (log2 ratio) -1
0
1
2 at 108.699 kb on - strand, within GFF2585 at 108.767 kb on - strand, within GFF2585 at 108.822 kb on + strand, within GFF2585 at 108.822 kb on + strand, within GFF2585 at 108.823 kb on - strand, within GFF2585 at 108.825 kb on - strand, within GFF2585 at 109.061 kb on - strand, within GFF2585 at 109.098 kb on - strand, within GFF2585 at 109.098 kb on - strand, within GFF2585 at 109.108 kb on + strand, within GFF2585 at 109.183 kb on + strand, within GFF2585 at 109.184 kb on - strand, within GFF2585 at 109.204 kb on + strand, within GFF2585 at 109.258 kb on + strand, within GFF2585 at 109.259 kb on - strand, within GFF2585 at 109.284 kb on - strand, within GFF2585 at 109.292 kb on + strand, within GFF2585 at 109.374 kb on - strand, within GFF2585 at 109.457 kb on + strand at 109.558 kb on + strand at 109.558 kb on + strand at 109.615 kb on + strand at 109.670 kb on + strand at 109.683 kb on + strand at 109.799 kb on - strand at 109.958 kb on + strand, within GFF2586 at 109.964 kb on + strand, within GFF2586 at 109.965 kb on - strand, within GFF2586 at 110.276 kb on - strand, within GFF2586 at 110.276 kb on - strand, within GFF2586 at 110.407 kb on + strand, within GFF2586 at 110.827 kb on - strand, within GFF2586 at 111.007 kb on + strand, within GFF2586 at 111.008 kb on - strand, within GFF2586 at 111.070 kb on - strand, within GFF2586 at 111.480 kb on - strand, within GFF2586 at 111.563 kb on - strand, within GFF2586 at 111.725 kb on + strand at 111.725 kb on + strand at 111.726 kb on - strand at 111.746 kb on + strand at 111.747 kb on - strand at 111.747 kb on - strand at 111.816 kb on + strand at 112.095 kb on - strand at 112.135 kb on + strand at 112.135 kb on + strand at 112.215 kb on - strand at 112.217 kb on + strand at 112.217 kb on + strand at 112.218 kb on - strand at 112.218 kb on - strand at 112.220 kb on - strand at 112.284 kb on + strand at 112.501 kb on + strand at 112.501 kb on + strand at 112.501 kb on + strand at 112.513 kb on - strand at 112.577 kb on + strand at 112.580 kb on + strand at 112.613 kb on + strand, within GFF2588 at 112.719 kb on + strand, within GFF2588 at 112.722 kb on - strand, within GFF2588 at 112.722 kb on - strand, within GFF2588 at 112.722 kb on - strand, within GFF2588 at 112.722 kb on - strand, within GFF2588 at 112.802 kb on - strand, within GFF2588 at 112.802 kb on - strand, within GFF2588
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 108,699 - GFF2585 0.67 +0.7 108,767 - GFF2585 0.69 +0.7 108,822 + GFF2585 0.71 +0.4 108,822 + GFF2585 0.71 +0.8 108,823 - GFF2585 0.71 +0.2 108,825 - GFF2585 0.71 +0.3 109,061 - GFF2585 0.79 +1.1 109,098 - GFF2585 0.80 +0.4 109,098 - GFF2585 0.80 -0.1 109,108 + GFF2585 0.80 +0.2 109,183 + GFF2585 0.83 -0.2 109,184 - GFF2585 0.83 +0.7 109,204 + GFF2585 0.84 +0.8 109,258 + GFF2585 0.85 +0.5 109,259 - GFF2585 0.85 +0.8 109,284 - GFF2585 0.86 +0.7 109,292 + GFF2585 0.86 -0.4 109,374 - GFF2585 0.89 +0.4 109,457 + +0.6 109,558 + +0.9 109,558 + +0.7 109,615 + +0.0 109,670 + +1.0 109,683 + +1.2 109,799 - +0.7 109,958 + GFF2586 0.12 +1.5 109,964 + GFF2586 0.13 +0.5 109,965 - GFF2586 0.13 +0.0 110,276 - GFF2586 0.27 +0.7 110,276 - GFF2586 0.27 +0.6 110,407 + GFF2586 0.33 -0.4 110,827 - GFF2586 0.53 +0.7 111,007 + GFF2586 0.61 +0.5 111,008 - GFF2586 0.61 +0.1 111,070 - GFF2586 0.64 +0.7 111,480 - GFF2586 0.83 +0.4 111,563 - GFF2586 0.87 +0.4 111,725 + +0.4 111,725 + -0.2 111,726 - +1.2 111,746 + +0.4 111,747 - +0.6 111,747 - +0.2 111,816 + -1.1 112,095 - -0.7 112,135 + -0.6 112,135 + -0.7 112,215 - -0.3 112,217 + -0.0 112,217 + -1.2 112,218 - -1.3 112,218 - -0.2 112,220 - -0.1 112,284 + +0.4 112,501 + +0.6 112,501 + +0.2 112,501 + +0.2 112,513 - -0.1 112,577 + -0.5 112,580 + -0.4 112,613 + GFF2588 0.11 -0.4 112,719 + GFF2588 0.18 +0.4 112,722 - GFF2588 0.19 +0.6 112,722 - GFF2588 0.19 -1.0 112,722 - GFF2588 0.19 +1.8 112,722 - GFF2588 0.19 -0.3 112,802 - GFF2588 0.24 +0.7 112,802 - GFF2588 0.24 +0.1
Or see this region's nucleotide sequence