Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2584 and GFF2585 are separated by 4 nucleotides GFF2585 and GFF2586 overlap by 4 nucleotides
GFF2584 - Putative virulence effector protein, at 105,389 to 106,708
GFF2584
GFF2585 - SrfB, at 106,713 to 109,694
GFF2585
GFF2586 - Putative virulence factor, at 109,691 to 111,835
GFF2586
Position (kb)
106
107
108
109
110 Strain fitness (log2 ratio)
-1
0
1 at 105.720 kb on + strand, within GFF2584 at 106.080 kb on + strand, within GFF2584 at 106.096 kb on - strand, within GFF2584 at 106.096 kb on - strand, within GFF2584 at 106.348 kb on + strand, within GFF2584 at 106.483 kb on - strand, within GFF2584 at 106.483 kb on - strand, within GFF2584 at 106.485 kb on + strand, within GFF2584 at 106.485 kb on + strand, within GFF2584 at 106.486 kb on - strand, within GFF2584 at 106.486 kb on - strand, within GFF2584 at 106.613 kb on - strand at 106.712 kb on + strand at 106.734 kb on + strand at 107.043 kb on - strand, within GFF2585 at 107.066 kb on + strand, within GFF2585 at 107.141 kb on + strand, within GFF2585 at 107.420 kb on + strand, within GFF2585 at 107.420 kb on + strand, within GFF2585 at 107.420 kb on + strand, within GFF2585 at 107.421 kb on - strand, within GFF2585 at 107.421 kb on - strand, within GFF2585 at 107.477 kb on + strand, within GFF2585 at 107.592 kb on - strand, within GFF2585 at 107.969 kb on + strand, within GFF2585 at 108.239 kb on - strand, within GFF2585 at 108.278 kb on - strand, within GFF2585 at 108.285 kb on + strand, within GFF2585 at 108.287 kb on + strand, within GFF2585 at 108.455 kb on - strand, within GFF2585 at 108.486 kb on - strand, within GFF2585 at 108.546 kb on - strand, within GFF2585 at 108.699 kb on - strand, within GFF2585 at 108.767 kb on - strand, within GFF2585 at 108.822 kb on + strand, within GFF2585 at 108.822 kb on + strand, within GFF2585 at 108.823 kb on - strand, within GFF2585 at 108.825 kb on - strand, within GFF2585 at 109.061 kb on - strand, within GFF2585 at 109.098 kb on - strand, within GFF2585 at 109.098 kb on - strand, within GFF2585 at 109.108 kb on + strand, within GFF2585 at 109.183 kb on + strand, within GFF2585 at 109.184 kb on - strand, within GFF2585 at 109.204 kb on + strand, within GFF2585 at 109.258 kb on + strand, within GFF2585 at 109.259 kb on - strand, within GFF2585 at 109.284 kb on - strand, within GFF2585 at 109.292 kb on + strand, within GFF2585 at 109.374 kb on - strand, within GFF2585 at 109.457 kb on + strand at 109.558 kb on + strand at 109.558 kb on + strand at 109.615 kb on + strand at 109.670 kb on + strand at 109.683 kb on + strand at 109.799 kb on - strand at 109.958 kb on + strand, within GFF2586 at 109.964 kb on + strand, within GFF2586 at 109.965 kb on - strand, within GFF2586 at 110.276 kb on - strand, within GFF2586 at 110.276 kb on - strand, within GFF2586 at 110.407 kb on + strand, within GFF2586
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 105,720 + GFF2584 0.25 +0.4 106,080 + GFF2584 0.52 +0.1 106,096 - GFF2584 0.54 +0.0 106,096 - GFF2584 0.54 -0.2 106,348 + GFF2584 0.73 +0.2 106,483 - GFF2584 0.83 +0.6 106,483 - GFF2584 0.83 +0.9 106,485 + GFF2584 0.83 +0.6 106,485 + GFF2584 0.83 +0.7 106,486 - GFF2584 0.83 +0.7 106,486 - GFF2584 0.83 +0.6 106,613 - +0.8 106,712 + +0.9 106,734 + +0.1 107,043 - GFF2585 0.11 +0.3 107,066 + GFF2585 0.12 +0.6 107,141 + GFF2585 0.14 -0.0 107,420 + GFF2585 0.24 +0.8 107,420 + GFF2585 0.24 +0.5 107,420 + GFF2585 0.24 +0.7 107,421 - GFF2585 0.24 +1.0 107,421 - GFF2585 0.24 -0.5 107,477 + GFF2585 0.26 +0.8 107,592 - GFF2585 0.29 +0.9 107,969 + GFF2585 0.42 +0.7 108,239 - GFF2585 0.51 +0.1 108,278 - GFF2585 0.52 +1.0 108,285 + GFF2585 0.53 +0.3 108,287 + GFF2585 0.53 +0.3 108,455 - GFF2585 0.58 +1.0 108,486 - GFF2585 0.59 +1.1 108,546 - GFF2585 0.61 +0.6 108,699 - GFF2585 0.67 +0.7 108,767 - GFF2585 0.69 +0.7 108,822 + GFF2585 0.71 +0.4 108,822 + GFF2585 0.71 +0.8 108,823 - GFF2585 0.71 +0.2 108,825 - GFF2585 0.71 +0.3 109,061 - GFF2585 0.79 +1.1 109,098 - GFF2585 0.80 +0.4 109,098 - GFF2585 0.80 -0.1 109,108 + GFF2585 0.80 +0.2 109,183 + GFF2585 0.83 -0.2 109,184 - GFF2585 0.83 +0.7 109,204 + GFF2585 0.84 +0.8 109,258 + GFF2585 0.85 +0.5 109,259 - GFF2585 0.85 +0.8 109,284 - GFF2585 0.86 +0.7 109,292 + GFF2585 0.86 -0.4 109,374 - GFF2585 0.89 +0.4 109,457 + +0.6 109,558 + +0.9 109,558 + +0.7 109,615 + +0.0 109,670 + +1.0 109,683 + +1.2 109,799 - +0.7 109,958 + GFF2586 0.12 +1.5 109,964 + GFF2586 0.13 +0.5 109,965 - GFF2586 0.13 +0.0 110,276 - GFF2586 0.27 +0.7 110,276 - GFF2586 0.27 +0.6 110,407 + GFF2586 0.33 -0.4
Or see this region's nucleotide sequence