Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2301 and GFF2302 are separated by 91 nucleotides GFF2302 and GFF2303 are separated by 112 nucleotides GFF2303 and GFF2304 are separated by 100 nucleotides GFF2304 and GFF2305 are separated by 106 nucleotides
GFF2301 - Ribulose-phosphate 3-epimerase (EC 5.1.3.1), at 88,333 to 89,097
GFF2301
GFF2302 - Triosephosphate isomerase (EC 5.3.1.1), at 89,189 to 89,956
GFF2302
GFF2303 - Putative uncharacterized protein YiiQ, at 90,069 to 90,665
GFF2303
GFF2304 - Putative membrane protein, at 90,766 to 91,194
GFF2304
GFF2305 - Ferredoxin--NADP(+) reductase (EC 1.18.1.2), at 91,301 to 92,047
GFF2305
Position (kb) 90
91 Strain fitness (log2 ratio) -1
0
1 at 89.082 kb on - strand at 89.095 kb on + strand at 89.096 kb on - strand at 89.096 kb on - strand at 89.121 kb on - strand at 89.130 kb on - strand at 89.133 kb on + strand at 89.133 kb on + strand at 89.190 kb on + strand at 89.683 kb on + strand, within GFF2302 at 89.986 kb on - strand at 90.033 kb on - strand at 90.161 kb on - strand, within GFF2303 at 90.161 kb on - strand, within GFF2303 at 90.161 kb on - strand, within GFF2303 at 90.287 kb on + strand, within GFF2303 at 90.288 kb on - strand, within GFF2303 at 90.288 kb on - strand, within GFF2303 at 90.289 kb on + strand, within GFF2303 at 90.290 kb on - strand, within GFF2303 at 90.290 kb on - strand, within GFF2303 at 90.374 kb on + strand, within GFF2303 at 90.530 kb on + strand, within GFF2303 at 90.566 kb on - strand, within GFF2303 at 90.640 kb on - strand at 90.730 kb on + strand at 90.752 kb on + strand at 90.752 kb on + strand at 90.753 kb on - strand at 91.325 kb on - strand at 91.563 kb on + strand, within GFF2305
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 89,082 - +0.3 89,095 + +0.4 89,096 - -0.3 89,096 - +0.2 89,121 - -0.2 89,130 - -1.5 89,133 + -0.2 89,133 + -0.5 89,190 + -1.0 89,683 + GFF2302 0.64 +0.5 89,986 - -0.4 90,033 - -0.2 90,161 - GFF2303 0.15 -0.4 90,161 - GFF2303 0.15 -1.3 90,161 - GFF2303 0.15 -0.6 90,287 + GFF2303 0.37 -1.0 90,288 - GFF2303 0.37 -1.3 90,288 - GFF2303 0.37 +0.3 90,289 + GFF2303 0.37 -0.8 90,290 - GFF2303 0.37 -1.3 90,290 - GFF2303 0.37 -1.4 90,374 + GFF2303 0.51 -0.8 90,530 + GFF2303 0.77 -0.6 90,566 - GFF2303 0.83 -0.9 90,640 - -1.1 90,730 + +0.8 90,752 + +0.1 90,752 + +1.4 90,753 - +1.3 91,325 - -0.4 91,563 + GFF2305 0.35 +1.5
Or see this region's nucleotide sequence