Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF227

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF226 and GFF227 overlap by 1 nucleotidesGFF227 and GFF228 are separated by 21 nucleotidesGFF228 and GFF229 overlap by 4 nucleotides GFF226 - Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives), at 756 to 1,955 GFF226 GFF227 - Homolog of E. coli HemX protein, at 1,955 to 3,124 GFF227 GFF228 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 3,146 to 3,886 GFF228 GFF229 - Porphobilinogen deaminase (EC 2.5.1.61), at 3,883 to 4,824 GFF229 Position (kb) 1 2 3 4Strain fitness (log2 ratio) -1 0 1 2at 1.112 kb on + strand, within GFF226at 1.113 kb on - strand, within GFF226at 1.329 kb on - strand, within GFF226at 1.362 kb on - strand, within GFF226at 1.363 kb on + strand, within GFF226at 1.364 kb on - strand, within GFF226at 1.597 kb on - strand, within GFF226at 1.767 kb on + strand, within GFF226at 1.768 kb on - strand, within GFF226at 1.768 kb on - strand, within GFF226at 1.771 kb on - strand, within GFF226at 1.788 kb on + strand, within GFF226at 1.789 kb on - strand, within GFF226at 1.850 kb on - strandat 1.850 kb on - strandat 1.873 kb on + strandat 1.874 kb on - strandat 1.874 kb on - strandat 1.938 kb on + strandat 2.039 kb on - strandat 2.176 kb on - strand, within GFF227at 2.176 kb on - strand, within GFF227at 2.289 kb on - strand, within GFF227at 2.292 kb on + strand, within GFF227at 2.293 kb on - strand, within GFF227at 2.354 kb on - strand, within GFF227at 2.573 kb on - strand, within GFF227at 2.753 kb on - strand, within GFF227at 2.823 kb on - strand, within GFF227at 2.971 kb on + strand, within GFF227at 3.011 kb on + strandat 3.011 kb on + strandat 3.147 kb on + strandat 3.147 kb on + strandat 3.153 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
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1,112 + GFF226 0.30 +1.3
1,113 - GFF226 0.30 +0.2
1,329 - GFF226 0.48 +0.3
1,362 - GFF226 0.51 +0.3
1,363 + GFF226 0.51 +0.8
1,364 - GFF226 0.51 +1.4
1,597 - GFF226 0.70 +0.6
1,767 + GFF226 0.84 +0.6
1,768 - GFF226 0.84 +0.8
1,768 - GFF226 0.84 +1.4
1,771 - GFF226 0.85 +0.1
1,788 + GFF226 0.86 +0.5
1,789 - GFF226 0.86 +2.0
1,850 - +0.9
1,850 - +0.5
1,873 + +0.3
1,874 - +0.9
1,874 - +0.7
1,938 + +0.1
2,039 - +0.2
2,176 - GFF227 0.19 +0.9
2,176 - GFF227 0.19 +0.6
2,289 - GFF227 0.29 +0.6
2,292 + GFF227 0.29 -0.3
2,293 - GFF227 0.29 +1.1
2,354 - GFF227 0.34 +1.9
2,573 - GFF227 0.53 +0.8
2,753 - GFF227 0.68 +0.7
2,823 - GFF227 0.74 +0.8
2,971 + GFF227 0.87 +1.0
3,011 + +0.7
3,011 + +0.1
3,147 + +2.1
3,147 + +0.9
3,153 - +0.7

Or see this region's nucleotide sequence