Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF278 and GFF226 are separated by 581 nucleotides GFF226 and GFF227 overlap by 1 nucleotides
GFF278 - tRNA-Pro-TGG, at 98 to 174
GFF278
GFF226 - Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives), at 756 to 1,955
GFF226
GFF227 - Homolog of E. coli HemX protein, at 1,955 to 3,124
GFF227
Position (kb) 0
1
2 Strain fitness (log2 ratio) -1
0
1
2 at 0.093 kb on + strand at 0.471 kb on + strand at 0.524 kb on - strand at 0.583 kb on + strand at 0.632 kb on - strand at 0.778 kb on - strand at 0.863 kb on - strand at 0.863 kb on - strand at 0.863 kb on - strand at 1.112 kb on + strand, within GFF226 at 1.113 kb on - strand, within GFF226 at 1.329 kb on - strand, within GFF226 at 1.362 kb on - strand, within GFF226 at 1.363 kb on + strand, within GFF226 at 1.364 kb on - strand, within GFF226 at 1.597 kb on - strand, within GFF226 at 1.767 kb on + strand, within GFF226 at 1.768 kb on - strand, within GFF226 at 1.768 kb on - strand, within GFF226 at 1.771 kb on - strand, within GFF226 at 1.788 kb on + strand, within GFF226 at 1.789 kb on - strand, within GFF226 at 1.850 kb on - strand at 1.850 kb on - strand at 1.873 kb on + strand at 1.874 kb on - strand at 1.874 kb on - strand at 1.938 kb on + strand at 2.039 kb on - strand at 2.176 kb on - strand, within GFF227 at 2.176 kb on - strand, within GFF227 at 2.289 kb on - strand, within GFF227 at 2.292 kb on + strand, within GFF227 at 2.293 kb on - strand, within GFF227 at 2.354 kb on - strand, within GFF227 at 2.573 kb on - strand, within GFF227 at 2.753 kb on - strand, within GFF227 at 2.823 kb on - strand, within GFF227
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 93 + +0.2 471 + -0.5 524 - +0.1 583 + +0.2 632 - +0.4 778 - +0.6 863 - -0.4 863 - -0.3 863 - +0.4 1,112 + GFF226 0.30 +1.3 1,113 - GFF226 0.30 +0.2 1,329 - GFF226 0.48 +0.3 1,362 - GFF226 0.51 +0.3 1,363 + GFF226 0.51 +0.8 1,364 - GFF226 0.51 +1.4 1,597 - GFF226 0.70 +0.6 1,767 + GFF226 0.84 +0.6 1,768 - GFF226 0.84 +0.8 1,768 - GFF226 0.84 +1.4 1,771 - GFF226 0.85 +0.1 1,788 + GFF226 0.86 +0.5 1,789 - GFF226 0.86 +2.0 1,850 - +0.9 1,850 - +0.5 1,873 + +0.3 1,874 - +0.9 1,874 - +0.7 1,938 + +0.1 2,039 - +0.2 2,176 - GFF227 0.19 +0.9 2,176 - GFF227 0.19 +0.6 2,289 - GFF227 0.29 +0.6 2,292 + GFF227 0.29 -0.3 2,293 - GFF227 0.29 +1.1 2,354 - GFF227 0.34 +1.9 2,573 - GFF227 0.53 +0.8 2,753 - GFF227 0.68 +0.7 2,823 - GFF227 0.74 +0.8
Or see this region's nucleotide sequence