Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2212

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2209 and GFF2210 overlap by 19 nucleotidesGFF2210 and GFF2211 are separated by 69 nucleotidesGFF2211 and GFF2212 are separated by 76 nucleotidesGFF2212 and GFF2213 are separated by 16 nucleotidesGFF2213 and GFF2214 are separated by 12 nucleotides GFF2209 - Molybdopterin-guanine dinucleotide biosynthesis protein MobB, at 608 to 1,138 GFF2209 GFF2210 - Molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 1,120 to 1,704 GFF2210 GFF2211 - Protein yihD, at 1,774 to 2,043 GFF2211 GFF2212 - YihE protein, a ser/thr kinase implicated in LPS synthesis and Cpx signalling, at 2,120 to 3,106 GFF2212 GFF2213 - Periplasmic thiol:disulfide interchange protein DsbA, at 3,123 to 3,746 GFF2213 GFF2214 - 1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 3,759 to 4,622 GFF2214 Position (kb) 2 3 4Strain fitness (log2 ratio) -2 -1 0 1 2at 1.182 kb on + strand, within GFF2210at 1.183 kb on - strand, within GFF2210at 1.183 kb on - strand, within GFF2210at 1.259 kb on + strand, within GFF2210at 1.259 kb on + strand, within GFF2210at 1.259 kb on + strand, within GFF2210at 1.259 kb on + strand, within GFF2210at 1.260 kb on - strand, within GFF2210at 1.260 kb on - strand, within GFF2210at 1.290 kb on + strand, within GFF2210at 1.291 kb on - strand, within GFF2210at 1.435 kb on + strand, within GFF2210at 1.560 kb on + strand, within GFF2210at 1.684 kb on + strandat 1.685 kb on - strandat 1.687 kb on + strandat 1.688 kb on - strandat 1.688 kb on - strandat 1.720 kb on + strandat 1.720 kb on + strandat 1.842 kb on + strand, within GFF2211at 1.927 kb on + strand, within GFF2211at 1.928 kb on - strand, within GFF2211at 2.034 kb on + strandat 2.445 kb on + strand, within GFF2212at 2.502 kb on - strand, within GFF2212at 2.661 kb on + strand, within GFF2212at 2.661 kb on + strand, within GFF2212at 2.662 kb on - strand, within GFF2212at 2.945 kb on + strand, within GFF2212at 2.946 kb on - strand, within GFF2212at 3.152 kb on + strandat 3.167 kb on + strandat 3.206 kb on + strand, within GFF2213at 3.206 kb on + strand, within GFF2213at 3.264 kb on + strand, within GFF2213at 3.428 kb on - strand, within GFF2213at 3.435 kb on - strand, within GFF2213at 3.482 kb on - strand, within GFF2213at 3.482 kb on - strand, within GFF2213at 3.482 kb on - strand, within GFF2213at 3.541 kb on - strand, within GFF2213at 3.660 kb on - strand, within GFF2213at 3.798 kb on + strandat 3.828 kb on - strandat 3.830 kb on + strandat 3.951 kb on - strand, within GFF2214at 3.956 kb on - strand, within GFF2214at 3.993 kb on - strand, within GFF2214at 3.993 kb on - strand, within GFF2214at 4.032 kb on - strand, within GFF2214

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
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1,182 + GFF2210 0.11 +0.7
1,183 - GFF2210 0.11 +1.0
1,183 - GFF2210 0.11 +0.7
1,259 + GFF2210 0.24 +0.4
1,259 + GFF2210 0.24 -0.0
1,259 + GFF2210 0.24 +0.5
1,259 + GFF2210 0.24 +0.5
1,260 - GFF2210 0.24 +0.2
1,260 - GFF2210 0.24 +0.5
1,290 + GFF2210 0.29 +0.6
1,291 - GFF2210 0.29 +0.7
1,435 + GFF2210 0.54 +0.9
1,560 + GFF2210 0.75 +0.4
1,684 + -0.6
1,685 - -0.2
1,687 + +1.3
1,688 - +0.5
1,688 - +0.2
1,720 + -0.0
1,720 + -0.5
1,842 + GFF2211 0.25 -0.3
1,927 + GFF2211 0.57 -0.0
1,928 - GFF2211 0.57 +0.2
2,034 + -0.3
2,445 + GFF2212 0.33 +0.1
2,502 - GFF2212 0.39 +0.5
2,661 + GFF2212 0.55 -1.9
2,661 + GFF2212 0.55 -1.9
2,662 - GFF2212 0.55 -1.0
2,945 + GFF2212 0.84 -0.7
2,946 - GFF2212 0.84 -0.7
3,152 + +0.8
3,167 + +1.1
3,206 + GFF2213 0.13 +0.7
3,206 + GFF2213 0.13 +1.6
3,264 + GFF2213 0.23 +1.8
3,428 - GFF2213 0.49 +0.8
3,435 - GFF2213 0.50 +1.4
3,482 - GFF2213 0.58 +0.7
3,482 - GFF2213 0.58 +0.8
3,482 - GFF2213 0.58 +0.9
3,541 - GFF2213 0.67 +2.1
3,660 - GFF2213 0.86 +1.2
3,798 + -0.2
3,828 - -0.0
3,830 + -0.3
3,951 - GFF2214 0.22 -0.1
3,956 - GFF2214 0.23 -0.5
3,993 - GFF2214 0.27 -0.6
3,993 - GFF2214 0.27 -0.5
4,032 - GFF2214 0.32 -0.1

Or see this region's nucleotide sequence