Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2135 and GFF2136 overlap by 1 nucleotides GFF2136 and GFF2137 are separated by 8 nucleotides GFF2137 and GFF2138 overlap by 11 nucleotides
GFF2135 - Rod shape-determining protein MreC, at 23,468 to 24,520
GFF2135
GFF2136 - Rod shape-determining protein MreD, at 24,520 to 25,011
GFF2136
GFF2137 - Septum formation protein Maf, at 25,020 to 25,613
GFF2137
GFF2138 - Cytoplasmic axial filament protein CafA and Ribonuclease G (EC 3.1.4.-), at 25,603 to 27,072
GFF2138
Position (kb) 25
26 Strain fitness (log2 ratio) -1
0
1
2 at 25.149 kb on + strand, within GFF2137 at 25.317 kb on - strand, within GFF2137 at 25.317 kb on - strand, within GFF2137 at 25.419 kb on - strand, within GFF2137 at 25.455 kb on - strand, within GFF2137 at 25.507 kb on + strand, within GFF2137 at 25.507 kb on + strand, within GFF2137 at 25.508 kb on - strand, within GFF2137 at 25.519 kb on - strand, within GFF2137 at 25.519 kb on - strand, within GFF2137 at 25.555 kb on - strand at 25.589 kb on + strand at 25.623 kb on - strand at 25.686 kb on + strand at 25.727 kb on - strand at 25.727 kb on - strand at 25.779 kb on + strand, within GFF2138 at 25.821 kb on + strand, within GFF2138 at 25.866 kb on + strand, within GFF2138 at 26.107 kb on - strand, within GFF2138 at 26.107 kb on - strand, within GFF2138 at 26.190 kb on - strand, within GFF2138 at 26.318 kb on - strand, within GFF2138 at 26.318 kb on - strand, within GFF2138 at 26.347 kb on + strand, within GFF2138 at 26.367 kb on + strand, within GFF2138 at 26.380 kb on + strand, within GFF2138 at 26.381 kb on - strand, within GFF2138 at 26.449 kb on - strand, within GFF2138 at 26.449 kb on - strand, within GFF2138 at 26.511 kb on - strand, within GFF2138 at 26.511 kb on - strand, within GFF2138 at 26.514 kb on + strand, within GFF2138 at 26.514 kb on + strand, within GFF2138 at 26.545 kb on - strand, within GFF2138
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 25,149 + GFF2137 0.22 -0.3 25,317 - GFF2137 0.50 +0.2 25,317 - GFF2137 0.50 +0.8 25,419 - GFF2137 0.67 +1.2 25,455 - GFF2137 0.73 +0.8 25,507 + GFF2137 0.82 +0.0 25,507 + GFF2137 0.82 -0.0 25,508 - GFF2137 0.82 +0.9 25,519 - GFF2137 0.84 +1.0 25,519 - GFF2137 0.84 +1.1 25,555 - +0.9 25,589 + +0.2 25,623 - +1.3 25,686 + +0.6 25,727 - -0.4 25,727 - +1.1 25,779 + GFF2138 0.12 +0.4 25,821 + GFF2138 0.15 +1.0 25,866 + GFF2138 0.18 +0.9 26,107 - GFF2138 0.34 +1.6 26,107 - GFF2138 0.34 +0.8 26,190 - GFF2138 0.40 +1.0 26,318 - GFF2138 0.49 +0.8 26,318 - GFF2138 0.49 +0.6 26,347 + GFF2138 0.51 +0.7 26,367 + GFF2138 0.52 +1.0 26,380 + GFF2138 0.53 +0.6 26,381 - GFF2138 0.53 +0.4 26,449 - GFF2138 0.58 +0.7 26,449 - GFF2138 0.58 +2.5 26,511 - GFF2138 0.62 +0.8 26,511 - GFF2138 0.62 +0.6 26,514 + GFF2138 0.62 +1.1 26,514 + GFF2138 0.62 +0.8 26,545 - GFF2138 0.64 +1.1
Or see this region's nucleotide sequence