Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2132 and GFF2133 are separated by 203 nucleotides GFF2133 and GFF2134 are separated by 307 nucleotides
GFF2132 - Alcohol dehydrogenase (EC 1.1.1.1), at 18,934 to 19,908
GFF2132
GFF2133 - RNase E specificity factor CsrD, at 20,112 to 22,052
GFF2133
GFF2134 - Rod shape-determining protein MreB, at 22,360 to 23,403
GFF2134
Position (kb) 20
21
22
23 Strain fitness (log2 ratio) -2
-1
0
1
2 at 19.114 kb on + strand, within GFF2132 at 19.115 kb on - strand, within GFF2132 at 19.134 kb on + strand, within GFF2132 at 19.153 kb on - strand, within GFF2132 at 19.211 kb on + strand, within GFF2132 at 19.211 kb on + strand, within GFF2132 at 19.276 kb on + strand, within GFF2132 at 19.348 kb on - strand, within GFF2132 at 19.405 kb on - strand, within GFF2132 at 19.658 kb on - strand, within GFF2132 at 19.660 kb on + strand, within GFF2132 at 19.660 kb on + strand, within GFF2132 at 19.770 kb on + strand, within GFF2132 at 19.786 kb on - strand, within GFF2132 at 19.924 kb on + strand at 19.925 kb on - strand at 19.925 kb on - strand at 19.925 kb on - strand at 19.925 kb on - strand at 19.931 kb on + strand at 19.932 kb on - strand at 19.932 kb on - strand at 19.932 kb on - strand at 19.932 kb on - strand at 19.948 kb on + strand at 19.949 kb on - strand at 19.957 kb on + strand at 19.960 kb on - strand at 20.025 kb on + strand at 20.026 kb on - strand at 20.087 kb on + strand at 20.183 kb on - strand at 20.183 kb on - strand at 20.183 kb on - strand at 20.203 kb on - strand at 20.212 kb on + strand at 20.217 kb on + strand at 20.218 kb on - strand at 20.219 kb on + strand at 20.219 kb on + strand at 20.219 kb on + strand at 20.219 kb on + strand at 20.219 kb on + strand at 20.219 kb on + strand at 20.220 kb on - strand at 20.220 kb on - strand at 20.220 kb on - strand at 20.220 kb on - strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.224 kb on - strand at 20.224 kb on - strand at 20.282 kb on + strand at 20.282 kb on + strand at 20.283 kb on - strand at 20.283 kb on - strand at 20.421 kb on + strand, within GFF2133 at 20.422 kb on - strand, within GFF2133 at 20.447 kb on - strand, within GFF2133 at 20.479 kb on - strand, within GFF2133 at 20.677 kb on - strand, within GFF2133 at 20.713 kb on + strand, within GFF2133 at 20.714 kb on - strand, within GFF2133 at 20.714 kb on - strand, within GFF2133 at 20.966 kb on + strand, within GFF2133 at 20.988 kb on - strand, within GFF2133 at 20.988 kb on - strand, within GFF2133 at 20.991 kb on - strand, within GFF2133 at 21.151 kb on + strand, within GFF2133 at 21.152 kb on - strand, within GFF2133 at 21.152 kb on - strand, within GFF2133 at 21.264 kb on + strand, within GFF2133 at 21.265 kb on - strand, within GFF2133 at 21.389 kb on - strand, within GFF2133 at 21.417 kb on + strand, within GFF2133 at 21.417 kb on + strand, within GFF2133 at 21.450 kb on + strand, within GFF2133 at 21.450 kb on + strand, within GFF2133 at 21.450 kb on + strand, within GFF2133 at 21.451 kb on - strand, within GFF2133 at 21.490 kb on - strand, within GFF2133 at 21.490 kb on - strand, within GFF2133 at 21.715 kb on + strand, within GFF2133 at 21.716 kb on - strand, within GFF2133 at 21.880 kb on + strand at 21.880 kb on + strand at 22.006 kb on + strand at 22.006 kb on + strand at 22.141 kb on + strand at 22.141 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 19,114 + GFF2132 0.18 -0.3 19,115 - GFF2132 0.19 +0.2 19,134 + GFF2132 0.21 +0.3 19,153 - GFF2132 0.22 -1.1 19,211 + GFF2132 0.28 +1.5 19,211 + GFF2132 0.28 -0.0 19,276 + GFF2132 0.35 +0.4 19,348 - GFF2132 0.42 +0.1 19,405 - GFF2132 0.48 -0.1 19,658 - GFF2132 0.74 +1.7 19,660 + GFF2132 0.74 -0.3 19,660 + GFF2132 0.74 -0.3 19,770 + GFF2132 0.86 -0.4 19,786 - GFF2132 0.87 -0.0 19,924 + +0.2 19,925 - -0.9 19,925 - +0.4 19,925 - -0.9 19,925 - -1.0 19,931 + +0.4 19,932 - +0.0 19,932 - -0.4 19,932 - +1.5 19,932 - -0.3 19,948 + -1.1 19,949 - -0.2 19,957 + -0.9 19,960 - +0.5 20,025 + +0.1 20,026 - -2.2 20,087 + -0.1 20,183 - +0.2 20,183 - +0.8 20,183 - +0.8 20,203 - +0.6 20,212 + +0.9 20,217 + +0.7 20,218 - +0.7 20,219 + +1.0 20,219 + +0.9 20,219 + +0.9 20,219 + +0.9 20,219 + +0.4 20,219 + +1.0 20,220 - +1.2 20,220 - +1.3 20,220 - +0.2 20,220 - +1.5 20,223 + +1.3 20,223 + +0.8 20,223 + +0.8 20,224 - +1.0 20,224 - +0.7 20,282 + +1.9 20,282 + +0.8 20,283 - +1.3 20,283 - +1.1 20,421 + GFF2133 0.16 +0.8 20,422 - GFF2133 0.16 +0.8 20,447 - GFF2133 0.17 +1.2 20,479 - GFF2133 0.19 +1.2 20,677 - GFF2133 0.29 +1.3 20,713 + GFF2133 0.31 +0.7 20,714 - GFF2133 0.31 +1.5 20,714 - GFF2133 0.31 +0.8 20,966 + GFF2133 0.44 +0.3 20,988 - GFF2133 0.45 +1.1 20,988 - GFF2133 0.45 +0.9 20,991 - GFF2133 0.45 +1.1 21,151 + GFF2133 0.54 +0.9 21,152 - GFF2133 0.54 +1.1 21,152 - GFF2133 0.54 +1.5 21,264 + GFF2133 0.59 +0.5 21,265 - GFF2133 0.59 +1.0 21,389 - GFF2133 0.66 +0.8 21,417 + GFF2133 0.67 +1.2 21,417 + GFF2133 0.67 +0.2 21,450 + GFF2133 0.69 +1.4 21,450 + GFF2133 0.69 +1.2 21,450 + GFF2133 0.69 +0.9 21,451 - GFF2133 0.69 +1.1 21,490 - GFF2133 0.71 +0.2 21,490 - GFF2133 0.71 +0.4 21,715 + GFF2133 0.83 +0.6 21,716 - GFF2133 0.83 +0.7 21,880 + +1.9 21,880 + +0.7 22,006 + -0.1 22,006 + +0.1 22,141 + +1.9 22,141 + +1.6
Or see this region's nucleotide sequence