Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1958 and GFF1959 are separated by 288 nucleotides GFF1959 and GFF1983 are separated by 75 nucleotides GFF1983 and GFF1960 are separated by 156 nucleotides
GFF1958 - Lipoprotein, at 150,153 to 150,908
GFF1958
GFF1959 - putative transport, at 151,197 to 152,138
GFF1959
GFF1983 - tRNA-Arg-CCT, at 152,214 to 152,288
GFF1983
GFF1960 - Protease VII (Omptin) precursor (EC 3.4.23.49), at 152,445 to 153,383
GFF1960
Position (kb) 151
152
153 Strain fitness (log2 ratio) -1
0
1 at 150.259 kb on + strand, within GFF1958 at 150.278 kb on + strand, within GFF1958 at 150.279 kb on - strand, within GFF1958 at 150.279 kb on - strand, within GFF1958 at 150.279 kb on - strand, within GFF1958 at 150.281 kb on - strand, within GFF1958 at 150.467 kb on + strand, within GFF1958 at 150.468 kb on - strand, within GFF1958 at 150.469 kb on + strand, within GFF1958 at 150.469 kb on + strand, within GFF1958 at 150.470 kb on - strand, within GFF1958 at 150.540 kb on - strand, within GFF1958 at 150.586 kb on - strand, within GFF1958 at 150.586 kb on - strand, within GFF1958 at 150.591 kb on - strand, within GFF1958 at 150.725 kb on - strand, within GFF1958 at 150.725 kb on - strand, within GFF1958 at 150.761 kb on - strand, within GFF1958 at 150.761 kb on - strand, within GFF1958 at 150.790 kb on + strand, within GFF1958 at 150.791 kb on - strand, within GFF1958 at 150.908 kb on + strand at 150.928 kb on - strand at 150.928 kb on - strand at 150.928 kb on - strand at 150.928 kb on - strand at 150.928 kb on - strand at 151.018 kb on + strand at 151.018 kb on + strand at 151.019 kb on - strand at 151.019 kb on - strand at 151.019 kb on - strand at 151.019 kb on - strand at 151.077 kb on - strand at 151.172 kb on + strand at 151.499 kb on + strand, within GFF1959 at 151.499 kb on + strand, within GFF1959 at 151.500 kb on - strand, within GFF1959 at 151.511 kb on + strand, within GFF1959 at 151.541 kb on - strand, within GFF1959 at 151.683 kb on + strand, within GFF1959 at 151.683 kb on + strand, within GFF1959 at 151.684 kb on - strand, within GFF1959 at 151.685 kb on + strand, within GFF1959 at 151.685 kb on + strand, within GFF1959 at 151.686 kb on - strand, within GFF1959 at 151.686 kb on - strand, within GFF1959 at 151.693 kb on + strand, within GFF1959 at 151.693 kb on + strand, within GFF1959 at 151.895 kb on + strand, within GFF1959 at 151.896 kb on - strand, within GFF1959 at 152.064 kb on - strand at 152.169 kb on + strand at 152.225 kb on + strand, within GFF1983 at 152.233 kb on + strand, within GFF1983 at 152.236 kb on - strand, within GFF1983 at 152.321 kb on - strand at 152.480 kb on - strand at 152.484 kb on - strand at 152.545 kb on - strand, within GFF1960 at 152.698 kb on + strand, within GFF1960 at 152.742 kb on - strand, within GFF1960 at 152.820 kb on - strand, within GFF1960 at 152.989 kb on + strand, within GFF1960 at 153.001 kb on + strand, within GFF1960
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 150,259 + GFF1958 0.14 +0.1 150,278 + GFF1958 0.17 +0.1 150,279 - GFF1958 0.17 +0.1 150,279 - GFF1958 0.17 +0.3 150,279 - GFF1958 0.17 -0.1 150,281 - GFF1958 0.17 +0.3 150,467 + GFF1958 0.42 +0.7 150,468 - GFF1958 0.42 +0.1 150,469 + GFF1958 0.42 -1.7 150,469 + GFF1958 0.42 +0.4 150,470 - GFF1958 0.42 +0.4 150,540 - GFF1958 0.51 +0.4 150,586 - GFF1958 0.57 -0.7 150,586 - GFF1958 0.57 -0.1 150,591 - GFF1958 0.58 +0.3 150,725 - GFF1958 0.76 -0.5 150,725 - GFF1958 0.76 +0.4 150,761 - GFF1958 0.80 -0.5 150,761 - GFF1958 0.80 +0.9 150,790 + GFF1958 0.84 +0.5 150,791 - GFF1958 0.84 -0.0 150,908 + +0.1 150,928 - +0.3 150,928 - -0.2 150,928 - -0.1 150,928 - +0.4 150,928 - -0.3 151,018 + -0.1 151,018 + -0.4 151,019 - +0.0 151,019 - -0.3 151,019 - -0.8 151,019 - -1.2 151,077 - -0.3 151,172 + -0.1 151,499 + GFF1959 0.32 -0.5 151,499 + GFF1959 0.32 -0.6 151,500 - GFF1959 0.32 -0.6 151,511 + GFF1959 0.33 -0.2 151,541 - GFF1959 0.37 +0.0 151,683 + GFF1959 0.52 +0.1 151,683 + GFF1959 0.52 -1.2 151,684 - GFF1959 0.52 +0.0 151,685 + GFF1959 0.52 -0.6 151,685 + GFF1959 0.52 -0.1 151,686 - GFF1959 0.52 +0.3 151,686 - GFF1959 0.52 +0.7 151,693 + GFF1959 0.53 -0.2 151,693 + GFF1959 0.53 -0.1 151,895 + GFF1959 0.74 -0.3 151,896 - GFF1959 0.74 -0.0 152,064 - -0.1 152,169 + +1.2 152,225 + GFF1983 0.15 +1.3 152,233 + GFF1983 0.25 +1.2 152,236 - GFF1983 0.29 +1.2 152,321 - -0.1 152,480 - +0.1 152,484 - +0.1 152,545 - GFF1960 0.11 -0.0 152,698 + GFF1960 0.27 +0.2 152,742 - GFF1960 0.32 +0.2 152,820 - GFF1960 0.40 +0.7 152,989 + GFF1960 0.58 -0.2 153,001 + GFF1960 0.59 -0.1
Or see this region's nucleotide sequence