Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF189 and GFF190 overlap by 4 nucleotides GFF190 and GFF191 are separated by 2 nucleotides GFF191 and GFF192 are separated by 10 nucleotides GFF192 and GFF193 are separated by 105 nucleotides
GFF189 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 184,620 to 185,609
GFF189
GFF190 - SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase (EC 2.1.1.182), at 185,606 to 186,427
GFF190
GFF191 - ApaG protein, at 186,430 to 186,807
GFF191
GFF192 - 'Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41)' transl_table=11, at 186,818 to 187,666
GFF192
GFF193 - Dihydrofolate reductase (EC 1.5.1.3), at 187,772 to 188,251
GFF193
Position (kb) 186
187 Strain fitness (log2 ratio) -1
0
1
2 at 185.438 kb on - strand, within GFF189 at 185.867 kb on + strand, within GFF190 at 185.957 kb on - strand, within GFF190 at 185.990 kb on + strand, within GFF190 at 185.991 kb on - strand, within GFF190 at 185.992 kb on + strand, within GFF190 at 186.020 kb on - strand, within GFF190 at 186.074 kb on + strand, within GFF190 at 186.074 kb on + strand, within GFF190 at 186.105 kb on - strand, within GFF190 at 186.392 kb on + strand at 186.585 kb on + strand, within GFF191 at 186.811 kb on + strand at 186.855 kb on - strand at 186.892 kb on + strand at 186.892 kb on + strand at 186.963 kb on + strand, within GFF192 at 186.964 kb on - strand, within GFF192 at 186.969 kb on - strand, within GFF192 at 186.989 kb on - strand, within GFF192 at 187.064 kb on - strand, within GFF192 at 187.183 kb on + strand, within GFF192 at 187.336 kb on - strand, within GFF192 at 187.346 kb on - strand, within GFF192 at 187.346 kb on - strand, within GFF192 at 187.348 kb on + strand, within GFF192 at 187.400 kb on + strand, within GFF192 at 187.478 kb on + strand, within GFF192 at 187.479 kb on - strand, within GFF192 at 187.479 kb on - strand, within GFF192 at 187.605 kb on - strand at 187.605 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 185,438 - GFF189 0.83 -0.2 185,867 + GFF190 0.32 +0.1 185,957 - GFF190 0.43 +0.6 185,990 + GFF190 0.47 -0.1 185,991 - GFF190 0.47 -0.2 185,992 + GFF190 0.47 +1.9 186,020 - GFF190 0.50 +0.5 186,074 + GFF190 0.57 +0.5 186,074 + GFF190 0.57 -0.3 186,105 - GFF190 0.61 -0.0 186,392 + -0.2 186,585 + GFF191 0.41 +0.7 186,811 + -0.5 186,855 - -0.7 186,892 + -0.0 186,892 + -0.5 186,963 + GFF192 0.17 -1.2 186,964 - GFF192 0.17 -0.4 186,969 - GFF192 0.18 -0.2 186,989 - GFF192 0.20 +0.2 187,064 - GFF192 0.29 +0.4 187,183 + GFF192 0.43 -0.5 187,336 - GFF192 0.61 -0.1 187,346 - GFF192 0.62 +0.0 187,346 - GFF192 0.62 +0.0 187,348 + GFF192 0.62 -0.7 187,400 + GFF192 0.69 -1.7 187,478 + GFF192 0.78 -0.0 187,479 - GFF192 0.78 +0.1 187,479 - GFF192 0.78 -0.1 187,605 - -0.1 187,605 - +0.2
Or see this region's nucleotide sequence