Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1527

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1524 and GFF1525 are separated by 3 nucleotidesGFF1525 and GFF1526 are separated by 30 nucleotidesGFF1526 and GFF1527 are separated by 14 nucleotidesGFF1527 and GFF1528 are separated by 197 nucleotides GFF1524 - PTS system, galactitol-specific IIC component (EC 2.7.1.69), at 28,287 to 29,660 GFF1524 GFF1525 - PTS system, galactitol-specific IIB component (EC 2.7.1.69), at 29,664 to 29,948 GFF1525 GFF1526 - PTS system, galactitol-specific IIA component (EC 2.7.1.69), at 29,979 to 30,443 GFF1526 GFF1527 - Tagatose-6-phosphate kinase GatZ (EC 2.7.1.144), at 30,458 to 31,729 GFF1527 GFF1528 - PTS system, tagatose-specific IIA-TPr component (EC 2.7.1.69), at 31,927 to 32,733 GFF1528 GFF1529 - PTS system, galactose-inducible IIB component (EC 2.7.1.69) / PTS system, galactose-inducible IIC component (EC 2.7.1.69), at 32,720 to 34,147 GFF1529 Position (kb) 30 31 32Strain fitness (log2 ratio) -2 -1 0 1at 29.534 kb on + strandat 29.549 kb on + strandat 29.638 kb on + strandat 29.784 kb on - strand, within GFF1525at 29.784 kb on - strand, within GFF1525at 29.790 kb on - strand, within GFF1525at 29.808 kb on - strand, within GFF1525at 29.810 kb on - strand, within GFF1525at 29.841 kb on + strand, within GFF1525at 29.856 kb on - strand, within GFF1525at 29.974 kb on + strandat 30.115 kb on - strand, within GFF1526at 30.223 kb on + strand, within GFF1526at 30.224 kb on - strand, within GFF1526at 30.341 kb on + strand, within GFF1526at 30.432 kb on + strandat 30.433 kb on - strandat 30.596 kb on + strand, within GFF1527at 30.597 kb on - strand, within GFF1527at 30.742 kb on - strand, within GFF1527at 30.770 kb on + strand, within GFF1527at 31.033 kb on + strand, within GFF1527at 31.234 kb on - strand, within GFF1527at 31.234 kb on - strand, within GFF1527at 31.392 kb on + strand, within GFF1527at 31.400 kb on + strand, within GFF1527at 31.403 kb on + strand, within GFF1527at 31.456 kb on + strand, within GFF1527at 31.457 kb on - strand, within GFF1527at 31.464 kb on - strand, within GFF1527at 31.857 kb on - strandat 32.044 kb on + strand, within GFF1528at 32.044 kb on + strand, within GFF1528at 32.044 kb on + strand, within GFF1528at 32.175 kb on + strand, within GFF1528at 32.200 kb on - strand, within GFF1528at 32.234 kb on - strand, within GFF1528at 32.356 kb on - strand, within GFF1528at 32.373 kb on + strand, within GFF1528at 32.457 kb on - strand, within GFF1528at 32.509 kb on + strand, within GFF1528at 32.509 kb on + strand, within GFF1528at 32.621 kb on + strand, within GFF1528

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
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29,534 + +0.2
29,549 + +0.5
29,638 + -0.5
29,784 - GFF1525 0.42 -0.1
29,784 - GFF1525 0.42 -0.3
29,790 - GFF1525 0.44 +0.3
29,808 - GFF1525 0.51 +0.7
29,810 - GFF1525 0.51 -1.2
29,841 + GFF1525 0.62 -0.0
29,856 - GFF1525 0.67 +0.3
29,974 + +0.8
30,115 - GFF1526 0.29 +0.0
30,223 + GFF1526 0.52 -0.5
30,224 - GFF1526 0.53 -0.2
30,341 + GFF1526 0.78 +0.2
30,432 + +0.1
30,433 - +0.1
30,596 + GFF1527 0.11 -2.1
30,597 - GFF1527 0.11 -0.4
30,742 - GFF1527 0.22 -0.2
30,770 + GFF1527 0.25 -0.7
31,033 + GFF1527 0.45 +0.0
31,234 - GFF1527 0.61 -0.6
31,234 - GFF1527 0.61 +0.7
31,392 + GFF1527 0.73 -0.2
31,400 + GFF1527 0.74 -0.6
31,403 + GFF1527 0.74 +0.2
31,456 + GFF1527 0.78 -0.3
31,457 - GFF1527 0.79 +0.1
31,464 - GFF1527 0.79 -0.1
31,857 - +0.1
32,044 + GFF1528 0.14 +0.0
32,044 + GFF1528 0.14 +0.2
32,044 + GFF1528 0.14 +0.8
32,175 + GFF1528 0.31 +0.1
32,200 - GFF1528 0.34 -0.1
32,234 - GFF1528 0.38 +0.4
32,356 - GFF1528 0.53 +1.0
32,373 + GFF1528 0.55 +0.3
32,457 - GFF1528 0.66 -0.1
32,509 + GFF1528 0.72 -0.4
32,509 + GFF1528 0.72 -0.1
32,621 + GFF1528 0.86 -0.2

Or see this region's nucleotide sequence