Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1445 and GFF1446 are separated by 149 nucleotides GFF1446 and GFF1447 are separated by 94 nucleotides
GFF1445 - Guanine-hypoxanthine permease, at 106,277 to 107,626
GFF1445
GFF1446 - Putative Na(+)/H(+) exchanger protein, CPA1 family precursor, at 107,776 to 109,422
GFF1446
GFF1447 - LysR family regulatory protein CidR, at 109,517 to 110,404
GFF1447
Position (kb)
107
108
109
110 Strain fitness (log2 ratio)
-1
0
1 at 106.831 kb on + strand, within GFF1445 at 106.856 kb on - strand, within GFF1445 at 106.906 kb on + strand, within GFF1445 at 106.918 kb on + strand, within GFF1445 at 107.035 kb on - strand, within GFF1445 at 107.126 kb on - strand, within GFF1445 at 107.235 kb on - strand, within GFF1445 at 107.355 kb on - strand, within GFF1445 at 107.355 kb on - strand, within GFF1445 at 107.467 kb on + strand, within GFF1445 at 107.468 kb on - strand, within GFF1445 at 107.483 kb on - strand, within GFF1445 at 107.555 kb on - strand at 107.625 kb on - strand at 107.625 kb on - strand at 107.795 kb on + strand at 107.795 kb on + strand at 107.831 kb on + strand at 107.832 kb on - strand at 107.844 kb on - strand at 107.844 kb on - strand at 107.872 kb on - strand at 108.304 kb on - strand, within GFF1446 at 108.628 kb on - strand, within GFF1446 at 108.746 kb on - strand, within GFF1446 at 108.746 kb on - strand, within GFF1446 at 108.915 kb on + strand, within GFF1446 at 108.915 kb on + strand, within GFF1446 at 108.916 kb on - strand, within GFF1446 at 108.924 kb on - strand, within GFF1446 at 109.194 kb on - strand, within GFF1446 at 109.403 kb on + strand at 109.404 kb on - strand at 109.506 kb on + strand at 109.582 kb on - strand at 109.766 kb on + strand, within GFF1447 at 109.767 kb on - strand, within GFF1447 at 109.767 kb on - strand, within GFF1447 at 109.784 kb on + strand, within GFF1447 at 109.785 kb on - strand, within GFF1447 at 109.785 kb on - strand, within GFF1447 at 109.825 kb on - strand, within GFF1447 at 109.828 kb on + strand, within GFF1447 at 109.829 kb on - strand, within GFF1447 at 109.829 kb on - strand, within GFF1447 at 109.829 kb on - strand, within GFF1447 at 109.829 kb on - strand, within GFF1447 at 110.013 kb on - strand, within GFF1447 at 110.152 kb on + strand, within GFF1447 at 110.240 kb on + strand, within GFF1447 at 110.241 kb on - strand, within GFF1447 at 110.264 kb on + strand, within GFF1447 at 110.270 kb on - strand, within GFF1447 at 110.275 kb on + strand, within GFF1447 at 110.276 kb on - strand, within GFF1447 at 110.276 kb on - strand, within GFF1447 at 110.330 kb on + strand at 110.382 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 106,831 + GFF1445 0.41 -0.5 106,856 - GFF1445 0.43 +1.0 106,906 + GFF1445 0.47 -0.3 106,918 + GFF1445 0.47 -0.4 107,035 - GFF1445 0.56 +0.8 107,126 - GFF1445 0.63 +0.5 107,235 - GFF1445 0.71 +0.2 107,355 - GFF1445 0.80 -0.2 107,355 - GFF1445 0.80 +0.0 107,467 + GFF1445 0.88 -0.2 107,468 - GFF1445 0.88 -0.2 107,483 - GFF1445 0.89 +0.0 107,555 - -0.0 107,625 - -0.6 107,625 - -0.0 107,795 + -0.9 107,795 + -0.4 107,831 + -0.2 107,832 - -0.9 107,844 - -0.2 107,844 - -1.0 107,872 - +0.3 108,304 - GFF1446 0.32 -0.2 108,628 - GFF1446 0.52 -0.2 108,746 - GFF1446 0.59 +1.0 108,746 - GFF1446 0.59 -0.0 108,915 + GFF1446 0.69 -0.5 108,915 + GFF1446 0.69 -0.4 108,916 - GFF1446 0.69 -0.4 108,924 - GFF1446 0.70 -0.6 109,194 - GFF1446 0.86 -0.2 109,403 + -0.5 109,404 - -0.0 109,506 + -0.2 109,582 - +0.5 109,766 + GFF1447 0.28 +0.4 109,767 - GFF1447 0.28 -0.0 109,767 - GFF1447 0.28 -0.4 109,784 + GFF1447 0.30 +0.1 109,785 - GFF1447 0.30 -0.0 109,785 - GFF1447 0.30 +0.1 109,825 - GFF1447 0.35 -0.9 109,828 + GFF1447 0.35 +0.8 109,829 - GFF1447 0.35 +0.0 109,829 - GFF1447 0.35 -0.6 109,829 - GFF1447 0.35 +0.0 109,829 - GFF1447 0.35 +0.6 110,013 - GFF1447 0.56 -0.1 110,152 + GFF1447 0.72 +0.4 110,240 + GFF1447 0.81 -0.1 110,241 - GFF1447 0.82 +0.1 110,264 + GFF1447 0.84 +0.2 110,270 - GFF1447 0.85 -0.4 110,275 + GFF1447 0.85 +0.7 110,276 - GFF1447 0.85 -0.7 110,276 - GFF1447 0.85 +0.1 110,330 + -0.5 110,382 + +0.2
Or see this region's nucleotide sequence