Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1058 and GFF1059 are separated by 117 nucleotides GFF1059 and GFF1060 are separated by 208 nucleotides
GFF1058 - NadR transcriptional regulator / Nicotinamide-nucleotide adenylyltransferase, NadR family (EC 2.7.7.1) / Ribosylnicotinamide kinase (EC 2.7.1.22), at 239,161 to 240,393
GFF1058
GFF1059 - ABC transporter, ATP-binding protein, at 240,511 to 242,178
GFF1059
GFF1060 - Soluble lytic murein transglycosylase precursor (EC 3.2.1.-), at 242,387 to 244,324
GFF1060
Position (kb) 240
241
242
243 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 239.608 kb on + strand, within GFF1058 at 239.686 kb on + strand, within GFF1058 at 239.717 kb on - strand, within GFF1058 at 239.824 kb on - strand, within GFF1058 at 239.856 kb on + strand, within GFF1058 at 239.948 kb on - strand, within GFF1058 at 239.959 kb on + strand, within GFF1058 at 239.960 kb on - strand, within GFF1058 at 240.170 kb on - strand, within GFF1058 at 240.201 kb on + strand, within GFF1058 at 240.201 kb on + strand, within GFF1058 at 240.428 kb on - strand at 240.523 kb on - strand at 240.591 kb on - strand at 240.690 kb on + strand, within GFF1059 at 240.691 kb on - strand, within GFF1059 at 240.895 kb on + strand, within GFF1059 at 240.895 kb on + strand, within GFF1059 at 240.895 kb on + strand, within GFF1059 at 240.895 kb on + strand, within GFF1059 at 240.895 kb on + strand, within GFF1059 at 241.242 kb on + strand, within GFF1059 at 241.448 kb on + strand, within GFF1059 at 241.448 kb on + strand, within GFF1059 at 241.448 kb on + strand, within GFF1059 at 241.448 kb on + strand, within GFF1059 at 241.468 kb on - strand, within GFF1059 at 241.521 kb on - strand, within GFF1059 at 241.533 kb on + strand, within GFF1059 at 241.533 kb on + strand, within GFF1059 at 241.764 kb on + strand, within GFF1059 at 241.847 kb on + strand, within GFF1059 at 241.940 kb on - strand, within GFF1059 at 242.041 kb on - strand at 242.118 kb on + strand at 242.194 kb on + strand at 242.200 kb on + strand at 242.215 kb on + strand at 242.216 kb on - strand at 242.252 kb on + strand at 242.281 kb on + strand at 242.282 kb on - strand at 242.433 kb on + strand at 242.433 kb on + strand at 242.506 kb on + strand at 242.541 kb on + strand at 242.542 kb on - strand at 242.542 kb on - strand at 242.786 kb on - strand, within GFF1060 at 242.789 kb on + strand, within GFF1060 at 242.790 kb on - strand, within GFF1060 at 242.790 kb on - strand, within GFF1060 at 242.936 kb on + strand, within GFF1060 at 242.947 kb on + strand, within GFF1060 at 242.974 kb on + strand, within GFF1060 at 243.017 kb on + strand, within GFF1060 at 243.052 kb on + strand, within GFF1060
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.0075 MOI remove 239,608 + GFF1058 0.36 -2.0 239,686 + GFF1058 0.43 -0.5 239,717 - GFF1058 0.45 -1.2 239,824 - GFF1058 0.54 -1.0 239,856 + GFF1058 0.56 -1.4 239,948 - GFF1058 0.64 -2.4 239,959 + GFF1058 0.65 -0.7 239,960 - GFF1058 0.65 -1.6 240,170 - GFF1058 0.82 -2.6 240,201 + GFF1058 0.84 -3.6 240,201 + GFF1058 0.84 -0.2 240,428 - -0.3 240,523 - +1.5 240,591 - +0.6 240,690 + GFF1059 0.11 +1.8 240,691 - GFF1059 0.11 +0.8 240,895 + GFF1059 0.23 +1.1 240,895 + GFF1059 0.23 +1.3 240,895 + GFF1059 0.23 +0.9 240,895 + GFF1059 0.23 +0.9 240,895 + GFF1059 0.23 +1.2 241,242 + GFF1059 0.44 +1.0 241,448 + GFF1059 0.56 +1.8 241,448 + GFF1059 0.56 +0.8 241,448 + GFF1059 0.56 +1.4 241,448 + GFF1059 0.56 +1.4 241,468 - GFF1059 0.57 +1.1 241,521 - GFF1059 0.61 +0.8 241,533 + GFF1059 0.61 +1.2 241,533 + GFF1059 0.61 +0.9 241,764 + GFF1059 0.75 +1.0 241,847 + GFF1059 0.80 +1.5 241,940 - GFF1059 0.86 +1.6 242,041 - +0.7 242,118 + +2.0 242,194 + +0.7 242,200 + +1.1 242,215 + +1.1 242,216 - +0.9 242,252 + +2.0 242,281 + -0.5 242,282 - +0.1 242,433 + +0.6 242,433 + -0.6 242,506 + -1.0 242,541 + -0.6 242,542 - +0.8 242,542 - -0.1 242,786 - GFF1060 0.21 -0.2 242,789 + GFF1060 0.21 +0.7 242,790 - GFF1060 0.21 +0.5 242,790 - GFF1060 0.21 +0.1 242,936 + GFF1060 0.28 -0.5 242,947 + GFF1060 0.29 -1.6 242,974 + GFF1060 0.30 -0.2 243,017 + GFF1060 0.33 +0.3 243,052 + GFF1060 0.34 -0.3
Or see this region's nucleotide sequence