Experiment: R2A outgrowth control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS28045 and ABID97_RS28050 are separated by 31 nucleotides ABID97_RS28050 and ABID97_RS28055 are separated by 47 nucleotides ABID97_RS28055 and ABID97_RS28060 overlap by 4 nucleotides
ABID97_RS28045: ABID97_RS28045 - hypothetical protein, at 526,991 to 527,266
_RS28045
ABID97_RS28050: ABID97_RS28050 - CBS domain-containing protein, at 527,298 to 527,735
_RS28050
ABID97_RS28055: ABID97_RS28055 - 2-oxoacid:ferredoxin oxidoreductase subunit beta, at 527,783 to 528,838
_RS28055
ABID97_RS28060: ABID97_RS28060 - 2-oxoacid:acceptor oxidoreductase subunit alpha, at 528,835 to 530,691
_RS28060
Position (kb) 527
528
529 Strain fitness (log2 ratio) -1
0
1
2 at 526.864 kb on + strand at 526.865 kb on - strand at 527.962 kb on - strand, within ABID97_RS28055 at 528.147 kb on + strand, within ABID97_RS28055 at 528.148 kb on - strand, within ABID97_RS28055 at 528.148 kb on - strand, within ABID97_RS28055 at 528.436 kb on - strand, within ABID97_RS28055 at 528.763 kb on - strand at 528.831 kb on + strand at 528.832 kb on - strand at 528.832 kb on - strand at 529.278 kb on - strand, within ABID97_RS28060 at 529.278 kb on - strand, within ABID97_RS28060 at 529.278 kb on - strand, within ABID97_RS28060 at 529.314 kb on + strand, within ABID97_RS28060 at 529.343 kb on + strand, within ABID97_RS28060 at 529.764 kb on - strand, within ABID97_RS28060
Per-strain Table
Position Strand Gene LocusTag Fraction R2A outgrowth control remove 526,864 + -0.5 526,865 - +0.2 527,962 - ABID97_RS28055 0.17 +2.6 528,147 + ABID97_RS28055 0.34 +0.1 528,148 - ABID97_RS28055 0.35 +1.3 528,148 - ABID97_RS28055 0.35 -0.2 528,436 - ABID97_RS28055 0.62 +0.7 528,763 - +0.7 528,831 + +0.7 528,832 - +0.1 528,832 - -0.6 529,278 - ABID97_RS28060 0.24 +0.0 529,278 - ABID97_RS28060 0.24 +0.3 529,278 - ABID97_RS28060 0.24 +0.1 529,314 + ABID97_RS28060 0.26 +0.5 529,343 + ABID97_RS28060 0.27 -0.2 529,764 - ABID97_RS28060 0.50 -0.3
Or see this region's nucleotide sequence