Experiment: R2A outgrowth control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS20160 and ABID97_RS20165 are separated by 159 nucleotides ABID97_RS20165 and ABID97_RS20170 are separated by 2 nucleotides ABID97_RS20170 and ABID97_RS20175 overlap by 1 nucleotides
ABID97_RS20160: ABID97_RS20160 - glycine zipper 2TM domain-containing protein, at 4,165,369 to 4,165,869
_RS20160
ABID97_RS20165: ABID97_RS20165 - dUTP diphosphatase, at 4,166,029 to 4,166,475
_RS20165
ABID97_RS20170: ABID97_RS20170 - hypothetical protein, at 4,166,478 to 4,166,927
_RS20170
ABID97_RS20175: ABID97_RS20175 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC, at 4,166,927 to 4,168,150
_RS20175
Position (kb)
4166
4167 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4165.488 kb on - strand, within ABID97_RS20160 at 4165.488 kb on - strand, within ABID97_RS20160 at 4165.718 kb on + strand, within ABID97_RS20160 at 4165.718 kb on + strand, within ABID97_RS20160 at 4165.718 kb on + strand, within ABID97_RS20160 at 4165.719 kb on - strand, within ABID97_RS20160 at 4165.719 kb on - strand, within ABID97_RS20160 at 4165.733 kb on + strand, within ABID97_RS20160 at 4165.782 kb on - strand, within ABID97_RS20160 at 4166.014 kb on + strand at 4166.479 kb on + strand at 4166.479 kb on + strand at 4166.479 kb on + strand at 4166.479 kb on + strand at 4166.649 kb on + strand, within ABID97_RS20170 at 4166.881 kb on + strand, within ABID97_RS20170 at 4166.882 kb on - strand, within ABID97_RS20170 at 4166.882 kb on - strand, within ABID97_RS20170 at 4166.882 kb on - strand, within ABID97_RS20170
Per-strain Table
Position Strand Gene LocusTag Fraction R2A outgrowth control remove 4,165,488 - ABID97_RS20160 0.24 -0.1 4,165,488 - ABID97_RS20160 0.24 +0.7 4,165,718 + ABID97_RS20160 0.70 -0.1 4,165,718 + ABID97_RS20160 0.70 +3.3 4,165,718 + ABID97_RS20160 0.70 +0.4 4,165,719 - ABID97_RS20160 0.70 +2.6 4,165,719 - ABID97_RS20160 0.70 +0.7 4,165,733 + ABID97_RS20160 0.73 +0.0 4,165,782 - ABID97_RS20160 0.82 -0.0 4,166,014 + +0.3 4,166,479 + +0.1 4,166,479 + +1.0 4,166,479 + -0.8 4,166,479 + +0.2 4,166,649 + ABID97_RS20170 0.38 +0.4 4,166,881 + ABID97_RS20170 0.90 -0.6 4,166,882 - ABID97_RS20170 0.90 -3.7 4,166,882 - ABID97_RS20170 0.90 +0.2 4,166,882 - ABID97_RS20170 0.90 +0.1
Or see this region's nucleotide sequence