Experiment: R2A outgrowth control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS18595 and ABID97_RS18600 are separated by 2 nucleotides ABID97_RS18600 and ABID97_RS18605 overlap by 4 nucleotides ABID97_RS18605 and ABID97_RS18610 are separated by 108 nucleotides
ABID97_RS18595: ABID97_RS18595 - 3-hydroxyacyl-CoA dehydrogenase, at 3,832,494 to 3,834,008
_RS18595
ABID97_RS18600: ABID97_RS18600 - enoyl-CoA hydratase-related protein, at 3,834,011 to 3,834,802
_RS18600
ABID97_RS18605: ABID97_RS18605 - enoyl-CoA hydratase-related protein, at 3,834,799 to 3,835,539
_RS18605
ABID97_RS18610: ABID97_RS18610 - long-chain fatty acid--CoA ligase, at 3,835,648 to 3,837,207
_RS18610
Position (kb) 3834
3835
3836 Strain fitness (log2 ratio) -1
0
1 at 3834.528 kb on + strand, within ABID97_RS18600 at 3835.608 kb on + strand at 3835.609 kb on - strand at 3835.609 kb on - strand at 3835.609 kb on - strand at 3835.609 kb on - strand at 3836.244 kb on - strand, within ABID97_RS18610 at 3836.259 kb on - strand, within ABID97_RS18610 at 3836.370 kb on - strand, within ABID97_RS18610
Per-strain Table
Position Strand Gene LocusTag Fraction R2A outgrowth control remove 3,834,528 + ABID97_RS18600 0.65 -0.8 3,835,608 + +0.2 3,835,609 - +0.8 3,835,609 - +0.3 3,835,609 - +0.0 3,835,609 - +0.3 3,836,244 - ABID97_RS18610 0.38 +0.9 3,836,259 - ABID97_RS18610 0.39 -1.1 3,836,370 - ABID97_RS18610 0.46 -1.4
Or see this region's nucleotide sequence