Experiment: toluene 2.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yggS and yggT are separated by 17 nucleotides yggT and yggU overlap by 4 nucleotides yggU and yggV are separated by 7 nucleotides yggV and yggW overlap by 8 nucleotides
b2951: yggS - predicted enzyme (NCBI), at 3,093,120 to 3,093,824
yggS
b2952: yggT - predicted inner membrane protein (NCBI), at 3,093,842 to 3,094,408
yggT
b2953: yggU - orf, hypothetical protein (VIMSS), at 3,094,405 to 3,094,695
yggU
b2954: yggV - putative deoxyribonucleotide triphosphate pyrophosphatase (NCBI), at 3,094,703 to 3,095,296
yggV
b2955: yggW - coproporphyrinogen III oxidase (NCBI), at 3,095,289 to 3,096,425
yggW
Position (kb)
3094
3095 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3093.416 kb on + strand, within yggS at 3093.416 kb on + strand, within yggS at 3093.416 kb on + strand, within yggS at 3093.604 kb on + strand, within yggS at 3093.604 kb on + strand, within yggS at 3093.675 kb on + strand, within yggS at 3093.729 kb on - strand, within yggS at 3093.855 kb on - strand at 3093.855 kb on - strand at 3093.883 kb on + strand at 3093.891 kb on - strand at 3093.930 kb on - strand, within yggT at 3093.930 kb on - strand, within yggT at 3093.940 kb on - strand, within yggT at 3094.022 kb on + strand, within yggT at 3094.067 kb on - strand, within yggT at 3094.089 kb on - strand, within yggT at 3094.089 kb on - strand, within yggT at 3094.290 kb on + strand, within yggT at 3094.319 kb on + strand, within yggT at 3094.324 kb on + strand, within yggT at 3094.333 kb on + strand, within yggT at 3094.333 kb on + strand, within yggT at 3094.391 kb on - strand at 3094.400 kb on + strand at 3094.400 kb on + strand at 3094.451 kb on + strand, within yggU at 3094.451 kb on + strand, within yggU at 3094.542 kb on + strand, within yggU at 3094.775 kb on + strand, within yggV at 3094.879 kb on + strand, within yggV at 3094.920 kb on - strand, within yggV at 3094.948 kb on + strand, within yggV at 3095.006 kb on + strand, within yggV at 3095.095 kb on - strand, within yggV at 3095.115 kb on + strand, within yggV at 3095.136 kb on + strand, within yggV at 3095.299 kb on - strand at 3095.380 kb on + strand at 3095.409 kb on - strand, within yggW at 3095.442 kb on - strand, within yggW at 3095.448 kb on + strand, within yggW at 3095.456 kb on - strand, within yggW at 3095.485 kb on + strand, within yggW at 3095.609 kb on - strand, within yggW at 3095.661 kb on - strand, within yggW
Per-strain Table
Position Strand Gene LocusTag Fraction toluene 2.5 vol% remove 3,093,416 + yggS b2951 0.42 +0.7 3,093,416 + yggS b2951 0.42 -0.1 3,093,416 + yggS b2951 0.42 +1.5 3,093,604 + yggS b2951 0.69 -0.3 3,093,604 + yggS b2951 0.69 +1.4 3,093,675 + yggS b2951 0.79 +0.6 3,093,729 - yggS b2951 0.86 -1.2 3,093,855 - +0.0 3,093,855 - +1.8 3,093,883 + -1.9 3,093,891 - +0.1 3,093,930 - yggT b2952 0.16 +1.7 3,093,930 - yggT b2952 0.16 -1.9 3,093,940 - yggT b2952 0.17 +0.6 3,094,022 + yggT b2952 0.32 -0.3 3,094,067 - yggT b2952 0.40 -1.0 3,094,089 - yggT b2952 0.44 -0.7 3,094,089 - yggT b2952 0.44 -2.0 3,094,290 + yggT b2952 0.79 +0.6 3,094,319 + yggT b2952 0.84 -1.0 3,094,324 + yggT b2952 0.85 -2.3 3,094,333 + yggT b2952 0.87 +0.3 3,094,333 + yggT b2952 0.87 -0.2 3,094,391 - -0.5 3,094,400 + +0.0 3,094,400 + +0.3 3,094,451 + yggU b2953 0.16 -1.0 3,094,451 + yggU b2953 0.16 -1.2 3,094,542 + yggU b2953 0.47 -0.8 3,094,775 + yggV b2954 0.12 +0.3 3,094,879 + yggV b2954 0.30 -1.6 3,094,920 - yggV b2954 0.37 +0.6 3,094,948 + yggV b2954 0.41 +0.4 3,095,006 + yggV b2954 0.51 -3.5 3,095,095 - yggV b2954 0.66 -0.3 3,095,115 + yggV b2954 0.69 -0.5 3,095,136 + yggV b2954 0.73 +0.1 3,095,299 - -0.2 3,095,380 + +1.1 3,095,409 - yggW b2955 0.11 +1.0 3,095,442 - yggW b2955 0.13 +0.3 3,095,448 + yggW b2955 0.14 -1.0 3,095,456 - yggW b2955 0.15 +0.4 3,095,485 + yggW b2955 0.17 -0.1 3,095,609 - yggW b2955 0.28 -1.9 3,095,661 - yggW b2955 0.33 -2.8
Or see this region's nucleotide sequence