Strain Fitness in Escherichia coli BW25113 around b2309

Experiment: toluene 2.5 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	toluene 2.5 vol%
2,423,038	+	hisM	b2307	0.70	-0.2
2,423,041	-	hisM	b2307	0.70	+0.0
2,423,145	+	hisM	b2307	0.85	+1.7
2,423,145	+	hisM	b2307	0.85	+0.5
2,423,199	-				+0.2
2,423,199	-				-1.3
2,423,233	-				-0.4
2,423,240	-				-1.2
2,423,240	-				+0.0
2,423,265	-				-1.4
2,423,265	-				-0.5
2,423,267	+				-0.0
2,423,280	+				-0.1
2,423,280	+				+0.2
2,423,310	-				-1.5
2,423,310	-				+1.4
2,423,346	+	hisQ	b2308	0.14	+0.5
2,423,421	-	hisQ	b2308	0.25	+0.5
2,423,426	+	hisQ	b2308	0.25	+0.4
2,423,490	+	hisQ	b2308	0.35	-0.1
2,423,566	+	hisQ	b2308	0.46	-0.4
2,423,572	-	hisQ	b2308	0.47	+0.5
2,423,641	+	hisQ	b2308	0.57	-0.8
2,423,670	+	hisQ	b2308	0.61	+0.3
2,423,696	+	hisQ	b2308	0.65	+0.8
2,423,696	+	hisQ	b2308	0.65	+0.8
2,423,702	-	hisQ	b2308	0.66	-0.8
2,423,702	-	hisQ	b2308	0.66	-0.6
2,423,752	-	hisQ	b2308	0.73	-0.6
2,423,779	+	hisQ	b2308	0.77	+0.4
2,423,786	-	hisQ	b2308	0.78	+0.5
2,423,830	-	hisQ	b2308	0.84	-0.8
2,423,830	-	hisQ	b2308	0.84	-0.9
2,423,866	+	hisQ	b2308	0.89	-0.2
2,423,866	+	hisQ	b2308	0.89	-0.4
2,423,866	+	hisQ	b2308	0.89	+0.2
2,423,866	+	hisQ	b2308	0.89	-0.5
2,423,866	+	hisQ	b2308	0.89	+0.5
2,423,940	+				-0.5
2,424,014	-				+0.7
2,424,158	-	hisJ	b2309	0.17	+2.3
2,424,158	-	hisJ	b2309	0.17	+1.9
2,424,158	-	hisJ	b2309	0.17	+1.2
2,424,239	-	hisJ	b2309	0.27	+0.4
2,424,383	+	hisJ	b2309	0.45	+1.3
2,424,384	-	hisJ	b2309	0.45	+0.6
2,424,406	-	hisJ	b2309	0.48	+1.5
2,424,863	-				-0.4
2,425,153	+	argT	b2310	0.16	+1.8
2,425,153	+	argT	b2310	0.16	-0.7
2,425,161	-	argT	b2310	0.17	+0.5
2,425,161	-	argT	b2310	0.17	-0.5
2,425,253	+	argT	b2310	0.28	-0.2
2,425,280	+	argT	b2310	0.32	-0.2
2,425,286	-	argT	b2310	0.33	-1.1
2,425,319	-	argT	b2310	0.37	-0.5
2,425,339	-	argT	b2310	0.39	+3.7
2,425,353	-	argT	b2310	0.41	-0.5
2,425,353	-	argT	b2310	0.41	-1.1
2,425,387	-	argT	b2310	0.45	+1.7
2,425,387	-	argT	b2310	0.45	+0.4
2,425,402	-	argT	b2310	0.47	-0.4
2,425,543	+	argT	b2310	0.65	+0.6
2,425,702	+	argT	b2310	0.86	-0.1
2,425,705	-	argT	b2310	0.86	-0.2
2,425,732	-	argT	b2310	0.90	-0.2
2,425,742	-				-0.2