Experiment: toluene 2.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yecN and yecO are separated by 40 nucleotides yecO and yecP overlap by 4 nucleotides yecP and bisZ are separated by 164 nucleotides
b1869: yecN - orf, hypothetical protein (VIMSS), at 1,950,290 to 1,950,685
yecN
b1870: yecO - predicted methyltransferase (NCBI), at 1,950,726 to 1,951,469
yecO
b1871: yecP - predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 1,951,466 to 1,952,437
yecP
b1872: bisZ - biotin sulfoxide reductase 2 (VIMSS), at 1,952,602 to 1,955,031
bisZ
Position (kb)
1951
1952
1953 Strain fitness (log2 ratio)
-2
-1
0
1 at 1950.475 kb on + strand, within yecN at 1950.475 kb on + strand, within yecN at 1950.509 kb on + strand, within yecN at 1950.517 kb on - strand, within yecN at 1950.525 kb on + strand, within yecN at 1950.525 kb on + strand, within yecN at 1950.612 kb on + strand, within yecN at 1950.612 kb on + strand, within yecN at 1950.613 kb on - strand, within yecN at 1950.613 kb on - strand, within yecN at 1950.743 kb on + strand at 1951.029 kb on + strand, within yecO at 1951.519 kb on - strand at 1951.579 kb on + strand, within yecP at 1951.831 kb on - strand, within yecP at 1951.831 kb on - strand, within yecP at 1951.834 kb on + strand, within yecP at 1951.834 kb on + strand, within yecP at 1951.868 kb on + strand, within yecP at 1951.912 kb on + strand, within yecP at 1951.912 kb on + strand, within yecP at 1951.920 kb on + strand, within yecP at 1952.017 kb on - strand, within yecP at 1952.017 kb on - strand, within yecP at 1952.288 kb on - strand, within yecP at 1952.330 kb on - strand, within yecP at 1952.330 kb on - strand, within yecP at 1952.419 kb on + strand at 1952.530 kb on + strand at 1952.786 kb on + strand at 1952.794 kb on - strand at 1952.794 kb on - strand at 1952.972 kb on + strand, within bisZ at 1952.986 kb on + strand, within bisZ at 1953.062 kb on - strand, within bisZ at 1953.078 kb on + strand, within bisZ at 1953.208 kb on - strand, within bisZ at 1953.262 kb on - strand, within bisZ at 1953.262 kb on - strand, within bisZ at 1953.262 kb on - strand, within bisZ at 1953.270 kb on + strand, within bisZ at 1953.298 kb on - strand, within bisZ
Per-strain Table
Position Strand Gene LocusTag Fraction toluene 2.5 vol% remove 1,950,475 + yecN b1869 0.47 +1.4 1,950,475 + yecN b1869 0.47 +0.3 1,950,509 + yecN b1869 0.55 -0.9 1,950,517 - yecN b1869 0.57 -1.4 1,950,525 + yecN b1869 0.59 -0.4 1,950,525 + yecN b1869 0.59 -0.1 1,950,612 + yecN b1869 0.81 +0.1 1,950,612 + yecN b1869 0.81 -0.0 1,950,613 - yecN b1869 0.82 +0.2 1,950,613 - yecN b1869 0.82 -0.1 1,950,743 + -0.0 1,951,029 + yecO b1870 0.41 +0.3 1,951,519 - +0.1 1,951,579 + yecP b1871 0.12 -0.0 1,951,831 - yecP b1871 0.38 +0.8 1,951,831 - yecP b1871 0.38 -0.4 1,951,834 + yecP b1871 0.38 -0.1 1,951,834 + yecP b1871 0.38 -0.7 1,951,868 + yecP b1871 0.41 -0.9 1,951,912 + yecP b1871 0.46 +1.2 1,951,912 + yecP b1871 0.46 -1.9 1,951,920 + yecP b1871 0.47 +0.1 1,952,017 - yecP b1871 0.57 -0.7 1,952,017 - yecP b1871 0.57 +1.4 1,952,288 - yecP b1871 0.85 +0.7 1,952,330 - yecP b1871 0.89 -1.2 1,952,330 - yecP b1871 0.89 -0.7 1,952,419 + +0.2 1,952,530 + -0.6 1,952,786 + -0.4 1,952,794 - +0.6 1,952,794 - -1.7 1,952,972 + bisZ b1872 0.15 +0.4 1,952,986 + bisZ b1872 0.16 -0.3 1,953,062 - bisZ b1872 0.19 -0.3 1,953,078 + bisZ b1872 0.20 +0.5 1,953,208 - bisZ b1872 0.25 -0.2 1,953,262 - bisZ b1872 0.27 -0.3 1,953,262 - bisZ b1872 0.27 +0.5 1,953,262 - bisZ b1872 0.27 +0.7 1,953,270 + bisZ b1872 0.27 -0.3 1,953,298 - bisZ b1872 0.29 -0.7
Or see this region's nucleotide sequence