Experiment: toluene 2.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafY and ykfL overlap by 1 nucleotides ykfL and ykfK are separated by 1 nucleotides ykfK and yafZ are separated by 216 nucleotides yafZ and ykfA are separated by 91 nucleotides
b0251: yafY - CP4-6 prophage; predicted DNA-binding transcriptional regulator (RefSeq), at 265,334 to 265,777
yafY
b4627: ykfL - no description, at 265,777 to 265,998
ykfL
b4628: ykfK - no description, at 266,000 to 266,191
ykfK
b0252: yafZ - orf, hypothetical protein (VIMSS), at 266,408 to 267,229
yafZ
b0253: ykfA - putative GTP-binding protein (VIMSS), at 267,321 to 268,184
ykfA
Position (kb) 266
267
268 Strain fitness (log2 ratio) -2
-1
0
1
2 at 265.426 kb on - strand, within yafY at 265.436 kb on - strand, within yafY at 265.519 kb on - strand, within yafY at 265.903 kb on + strand, within ykfL at 265.906 kb on - strand, within ykfL at 265.943 kb on - strand, within ykfL at 265.943 kb on - strand, within ykfL at 265.945 kb on + strand, within ykfL at 265.945 kb on + strand, within ykfL at 265.951 kb on - strand, within ykfL at 266.018 kb on + strand at 266.018 kb on + strand at 266.187 kb on + strand at 266.217 kb on - strand at 266.258 kb on + strand at 266.267 kb on - strand at 266.267 kb on - strand at 266.267 kb on - strand at 266.353 kb on - strand at 266.433 kb on + strand at 266.436 kb on - strand at 266.449 kb on + strand at 266.464 kb on - strand at 266.569 kb on - strand, within yafZ at 266.668 kb on - strand, within yafZ at 266.773 kb on - strand, within yafZ at 266.810 kb on - strand, within yafZ at 266.946 kb on - strand, within yafZ at 267.028 kb on - strand, within yafZ at 267.028 kb on - strand, within yafZ at 267.062 kb on - strand, within yafZ at 267.062 kb on - strand, within yafZ at 267.093 kb on + strand, within yafZ at 267.122 kb on + strand, within yafZ at 267.137 kb on + strand, within yafZ at 267.137 kb on + strand, within yafZ at 267.168 kb on - strand at 267.229 kb on + strand at 267.229 kb on + strand at 267.289 kb on + strand at 267.289 kb on + strand at 267.317 kb on + strand at 267.320 kb on - strand at 267.351 kb on - strand at 267.351 kb on - strand at 267.437 kb on - strand, within ykfA at 267.580 kb on - strand, within ykfA at 267.604 kb on + strand, within ykfA at 267.607 kb on - strand, within ykfA at 267.607 kb on - strand, within ykfA at 267.617 kb on - strand, within ykfA at 267.622 kb on + strand, within ykfA at 267.622 kb on + strand, within ykfA at 267.622 kb on + strand, within ykfA at 267.687 kb on - strand, within ykfA at 267.715 kb on - strand, within ykfA at 267.716 kb on + strand, within ykfA at 267.720 kb on - strand, within ykfA at 267.726 kb on + strand, within ykfA at 267.734 kb on - strand, within ykfA at 267.758 kb on - strand, within ykfA at 267.771 kb on - strand, within ykfA at 267.828 kb on + strand, within ykfA at 267.838 kb on - strand, within ykfA at 267.838 kb on - strand, within ykfA at 267.864 kb on - strand, within ykfA at 267.869 kb on - strand, within ykfA at 267.879 kb on - strand, within ykfA at 267.879 kb on - strand, within ykfA at 267.879 kb on - strand, within ykfA at 267.879 kb on - strand, within ykfA at 267.909 kb on + strand, within ykfA at 267.933 kb on - strand, within ykfA at 267.978 kb on - strand, within ykfA at 267.978 kb on - strand, within ykfA at 268.000 kb on + strand, within ykfA at 268.120 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction toluene 2.5 vol% remove 265,426 - yafY b0251 0.21 +0.6 265,436 - yafY b0251 0.23 +0.6 265,519 - yafY b0251 0.42 -0.7 265,903 + ykfL b4627 0.57 +1.8 265,906 - ykfL b4627 0.58 -0.7 265,943 - ykfL b4627 0.75 -0.9 265,943 - ykfL b4627 0.75 +0.2 265,945 + ykfL b4627 0.76 +2.0 265,945 + ykfL b4627 0.76 +0.0 265,951 - ykfL b4627 0.78 +1.4 266,018 + +0.9 266,018 + -0.4 266,187 + -1.0 266,217 - -0.1 266,258 + +0.1 266,267 - +0.6 266,267 - +0.5 266,267 - -0.3 266,353 - -0.6 266,433 + -0.1 266,436 - -2.0 266,449 + +0.7 266,464 - -1.1 266,569 - yafZ b0252 0.20 -0.0 266,668 - yafZ b0252 0.32 +0.5 266,773 - yafZ b0252 0.44 +0.7 266,810 - yafZ b0252 0.49 +0.7 266,946 - yafZ b0252 0.65 +0.4 267,028 - yafZ b0252 0.75 +0.4 267,028 - yafZ b0252 0.75 -1.3 267,062 - yafZ b0252 0.80 +0.2 267,062 - yafZ b0252 0.80 +1.2 267,093 + yafZ b0252 0.83 -0.3 267,122 + yafZ b0252 0.87 +0.4 267,137 + yafZ b0252 0.89 +1.8 267,137 + yafZ b0252 0.89 +2.3 267,168 - -1.4 267,229 + +0.6 267,229 + -2.6 267,289 + +0.5 267,289 + +0.5 267,317 + +0.4 267,320 - -0.4 267,351 - -0.5 267,351 - +0.2 267,437 - ykfA b0253 0.13 -0.5 267,580 - ykfA b0253 0.30 -0.1 267,604 + ykfA b0253 0.33 +0.7 267,607 - ykfA b0253 0.33 +0.4 267,607 - ykfA b0253 0.33 +0.7 267,617 - ykfA b0253 0.34 +0.5 267,622 + ykfA b0253 0.35 +0.1 267,622 + ykfA b0253 0.35 +1.0 267,622 + ykfA b0253 0.35 +0.3 267,687 - ykfA b0253 0.42 -1.8 267,715 - ykfA b0253 0.46 -0.6 267,716 + ykfA b0253 0.46 +0.0 267,720 - ykfA b0253 0.46 -0.2 267,726 + ykfA b0253 0.47 -1.9 267,734 - ykfA b0253 0.48 +0.2 267,758 - ykfA b0253 0.51 -0.2 267,771 - ykfA b0253 0.52 -0.1 267,828 + ykfA b0253 0.59 +0.4 267,838 - ykfA b0253 0.60 +0.6 267,838 - ykfA b0253 0.60 +0.2 267,864 - ykfA b0253 0.63 -0.4 267,869 - ykfA b0253 0.63 +2.7 267,879 - ykfA b0253 0.65 -0.9 267,879 - ykfA b0253 0.65 +0.4 267,879 - ykfA b0253 0.65 -0.8 267,879 - ykfA b0253 0.65 -0.4 267,909 + ykfA b0253 0.68 +1.2 267,933 - ykfA b0253 0.71 +0.1 267,978 - ykfA b0253 0.76 -2.1 267,978 - ykfA b0253 0.76 -0.9 268,000 + ykfA b0253 0.79 +1.0 268,120 + -1.3
Or see this region's nucleotide sequence