Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF763 and GFF764 are separated by 282 nucleotides GFF764 and GFF765 are separated by 29 nucleotides GFF765 and GFF766 are separated by 9 nucleotides
GFF763 - 23S rRNA (Uracil-5-) -methyltransferase rumB (EC 2.1.1.-), at 120,177 to 121,307
GFF763
GFF764 - Ascorbate-specific PTS system, EIIC component, at 121,590 to 122,933
GFF764
GFF765 - Putative phosphotransferase enzyme II, B component, at 122,963 to 123,283
GFF765
GFF766 - Choline-sulfatase (EC 3.1.6.6), at 123,293 to 124,780
GFF766
Position (kb)
121
122
123 Strain fitness (log2 ratio)
-1
0
1 at 120.590 kb on - strand, within GFF763 at 120.689 kb on + strand, within GFF763 at 120.704 kb on + strand, within GFF763 at 120.821 kb on + strand, within GFF763 at 120.838 kb on + strand, within GFF763 at 120.839 kb on - strand, within GFF763 at 121.093 kb on + strand, within GFF763 at 122.029 kb on + strand, within GFF764 at 122.123 kb on + strand, within GFF764 at 122.129 kb on + strand, within GFF764 at 122.159 kb on + strand, within GFF764 at 122.172 kb on - strand, within GFF764 at 122.372 kb on - strand, within GFF764 at 122.400 kb on + strand, within GFF764 at 122.406 kb on + strand, within GFF764 at 122.407 kb on - strand, within GFF764 at 122.439 kb on + strand, within GFF764 at 122.439 kb on + strand, within GFF764 at 122.439 kb on + strand, within GFF764 at 122.439 kb on + strand, within GFF764 at 123.329 kb on - strand at 123.415 kb on + strand at 123.416 kb on - strand at 123.419 kb on + strand at 123.419 kb on + strand at 123.439 kb on + strand at 123.440 kb on - strand at 123.440 kb on - strand at 123.555 kb on - strand, within GFF766 at 123.565 kb on + strand, within GFF766 at 123.622 kb on + strand, within GFF766 at 123.636 kb on + strand, within GFF766 at 123.774 kb on - strand, within GFF766 at 123.832 kb on + strand, within GFF766 at 123.864 kb on + strand, within GFF766 at 123.929 kb on + strand, within GFF766
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI remove 120,590 - GFF763 0.37 +0.5 120,689 + GFF763 0.45 +0.4 120,704 + GFF763 0.47 -0.2 120,821 + GFF763 0.57 -0.2 120,838 + GFF763 0.58 -0.8 120,839 - GFF763 0.59 -0.3 121,093 + GFF763 0.81 -0.4 122,029 + GFF764 0.33 +0.8 122,123 + GFF764 0.40 +0.5 122,129 + GFF764 0.40 -1.2 122,159 + GFF764 0.42 -0.0 122,172 - GFF764 0.43 +0.3 122,372 - GFF764 0.58 +0.4 122,400 + GFF764 0.60 +0.8 122,406 + GFF764 0.61 -0.0 122,407 - GFF764 0.61 +0.4 122,439 + GFF764 0.63 +0.4 122,439 + GFF764 0.63 +0.5 122,439 + GFF764 0.63 -1.1 122,439 + GFF764 0.63 +1.1 123,329 - +0.7 123,415 + +0.5 123,416 - -0.1 123,419 + -0.2 123,419 + -1.3 123,439 + +0.5 123,440 - -0.1 123,440 - +0.1 123,555 - GFF766 0.18 -0.3 123,565 + GFF766 0.18 +0.1 123,622 + GFF766 0.22 +0.1 123,636 + GFF766 0.23 +0.2 123,774 - GFF766 0.32 +0.7 123,832 + GFF766 0.36 -0.1 123,864 + GFF766 0.38 +0.0 123,929 + GFF766 0.43 -0.5
Or see this region's nucleotide sequence