Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4261 and GFF4262 are separated by 61 nucleotides GFF4262 and GFF4263 are separated by 29 nucleotides GFF4263 and GFF4264 are separated by 122 nucleotides
GFF4261 - Propionate catabolism operon regulatory protein PrpR, at 46,299 to 47,924
GFF4261
GFF4262 - FIG01048137: hypothetical protein, at 47,986 to 48,159
GFF4262
GFF4263 - Methylisocitrate lyase (EC 4.1.3.30), at 48,189 to 49,076
GFF4263
GFF4264 - 2-methylcitrate synthase (EC 2.3.3.5), at 49,199 to 50,368
GFF4264
Position (kb)
48
49
50 Strain fitness (log2 ratio)
-1
0
1 at 47.213 kb on - strand, within GFF4261 at 47.220 kb on - strand, within GFF4261 at 47.264 kb on + strand, within GFF4261 at 47.289 kb on + strand, within GFF4261 at 47.390 kb on + strand, within GFF4261 at 47.512 kb on + strand, within GFF4261 at 47.512 kb on + strand, within GFF4261 at 47.512 kb on + strand, within GFF4261 at 47.582 kb on - strand, within GFF4261 at 47.634 kb on + strand, within GFF4261 at 47.637 kb on + strand, within GFF4261 at 48.315 kb on + strand, within GFF4263 at 48.316 kb on - strand, within GFF4263 at 48.474 kb on - strand, within GFF4263 at 48.474 kb on - strand, within GFF4263 at 48.601 kb on + strand, within GFF4263 at 48.717 kb on + strand, within GFF4263 at 49.023 kb on + strand at 49.023 kb on + strand at 49.023 kb on + strand at 49.024 kb on - strand at 49.074 kb on + strand at 49.082 kb on - strand at 49.128 kb on - strand at 49.154 kb on + strand at 49.154 kb on + strand at 49.155 kb on - strand at 49.172 kb on - strand at 49.172 kb on - strand at 49.297 kb on + strand at 49.433 kb on + strand, within GFF4264 at 49.434 kb on - strand, within GFF4264 at 49.434 kb on - strand, within GFF4264 at 49.579 kb on + strand, within GFF4264 at 49.580 kb on - strand, within GFF4264 at 49.580 kb on - strand, within GFF4264 at 49.656 kb on - strand, within GFF4264 at 49.676 kb on - strand, within GFF4264 at 49.772 kb on - strand, within GFF4264 at 49.788 kb on - strand, within GFF4264
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI remove 47,213 - GFF4261 0.56 +0.2 47,220 - GFF4261 0.57 +0.3 47,264 + GFF4261 0.59 -1.2 47,289 + GFF4261 0.61 -0.0 47,390 + GFF4261 0.67 +0.3 47,512 + GFF4261 0.75 +0.5 47,512 + GFF4261 0.75 -0.2 47,512 + GFF4261 0.75 -0.1 47,582 - GFF4261 0.79 +0.1 47,634 + GFF4261 0.82 +0.8 47,637 + GFF4261 0.82 +0.1 48,315 + GFF4263 0.14 -0.2 48,316 - GFF4263 0.14 -0.1 48,474 - GFF4263 0.32 +0.1 48,474 - GFF4263 0.32 +1.0 48,601 + GFF4263 0.46 -1.4 48,717 + GFF4263 0.59 -1.0 49,023 + -1.1 49,023 + -0.8 49,023 + -0.1 49,024 - -0.5 49,074 + -0.6 49,082 - -0.3 49,128 - +0.2 49,154 + -0.6 49,154 + +0.5 49,155 - -0.1 49,172 - +0.0 49,172 - +0.4 49,297 + -0.0 49,433 + GFF4264 0.20 +0.4 49,434 - GFF4264 0.20 +0.0 49,434 - GFF4264 0.20 +0.8 49,579 + GFF4264 0.32 +0.2 49,580 - GFF4264 0.33 -0.1 49,580 - GFF4264 0.33 +0.1 49,656 - GFF4264 0.39 -0.1 49,676 - GFF4264 0.41 -0.3 49,772 - GFF4264 0.49 +0.4 49,788 - GFF4264 0.50 -0.4
Or see this region's nucleotide sequence