Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF422 and GFF423 are separated by 168 nucleotides GFF423 and GFF424 are separated by 117 nucleotides
GFF422 - Putative sugar kinase, at 142,517 to 143,398
GFF422
GFF423 - Aldehyde dehydrogenase B (EC 1.2.1.22), at 143,567 to 145,105
GFF423
GFF424 - Putative glycosyl hydrolase of unknown function (DUF1680), at 145,223 to 147,178
GFF424
Position (kb)
143
144
145
146 Strain fitness (log2 ratio)
-1
0
1
2 at 142.728 kb on + strand, within GFF422 at 142.869 kb on - strand, within GFF422 at 142.891 kb on + strand, within GFF422 at 142.892 kb on - strand, within GFF422 at 142.898 kb on - strand, within GFF422 at 142.982 kb on - strand, within GFF422 at 143.342 kb on - strand at 143.389 kb on - strand at 143.398 kb on + strand at 143.398 kb on + strand at 143.399 kb on - strand at 143.431 kb on + strand at 143.432 kb on - strand at 143.435 kb on - strand at 143.471 kb on - strand at 143.543 kb on + strand at 143.544 kb on - strand at 143.554 kb on + strand at 143.628 kb on + strand at 143.650 kb on + strand at 143.783 kb on - strand, within GFF423 at 143.806 kb on + strand, within GFF423 at 143.814 kb on + strand, within GFF423 at 143.938 kb on - strand, within GFF423 at 143.977 kb on + strand, within GFF423 at 143.978 kb on - strand, within GFF423 at 143.978 kb on - strand, within GFF423 at 143.978 kb on - strand, within GFF423 at 143.980 kb on - strand, within GFF423 at 144.336 kb on - strand, within GFF423 at 144.392 kb on - strand, within GFF423 at 144.392 kb on - strand, within GFF423 at 144.470 kb on - strand, within GFF423 at 144.487 kb on + strand, within GFF423 at 144.487 kb on + strand, within GFF423 at 144.488 kb on - strand, within GFF423 at 144.525 kb on - strand, within GFF423 at 144.595 kb on + strand, within GFF423 at 144.647 kb on + strand, within GFF423 at 144.648 kb on - strand, within GFF423 at 144.649 kb on + strand, within GFF423 at 144.721 kb on + strand, within GFF423 at 144.734 kb on + strand, within GFF423 at 144.746 kb on - strand, within GFF423 at 144.769 kb on + strand, within GFF423 at 144.824 kb on - strand, within GFF423 at 144.960 kb on - strand at 144.998 kb on - strand at 145.167 kb on - strand at 145.333 kb on - strand at 145.403 kb on - strand at 145.466 kb on - strand, within GFF424 at 145.567 kb on - strand, within GFF424 at 145.718 kb on + strand, within GFF424 at 145.785 kb on + strand, within GFF424 at 145.851 kb on + strand, within GFF424 at 145.852 kb on - strand, within GFF424 at 145.852 kb on - strand, within GFF424 at 145.852 kb on - strand, within GFF424 at 145.855 kb on + strand, within GFF424 at 145.856 kb on - strand, within GFF424 at 145.863 kb on + strand, within GFF424 at 145.864 kb on - strand, within GFF424 at 145.893 kb on + strand, within GFF424 at 145.894 kb on - strand, within GFF424 at 146.034 kb on + strand, within GFF424 at 146.062 kb on + strand, within GFF424 at 146.063 kb on - strand, within GFF424
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI remove 142,728 + GFF422 0.24 -0.1 142,869 - GFF422 0.40 +0.4 142,891 + GFF422 0.42 -1.4 142,892 - GFF422 0.43 +0.1 142,898 - GFF422 0.43 -0.4 142,982 - GFF422 0.53 -0.4 143,342 - +1.2 143,389 - +0.2 143,398 + -0.9 143,398 + -0.1 143,399 - +0.2 143,431 + +0.2 143,432 - -0.3 143,435 - +0.0 143,471 - -0.6 143,543 + +0.6 143,544 - -1.0 143,554 + +0.3 143,628 + +0.1 143,650 + +0.2 143,783 - GFF423 0.14 -0.7 143,806 + GFF423 0.16 +0.5 143,814 + GFF423 0.16 +0.5 143,938 - GFF423 0.24 -0.5 143,977 + GFF423 0.27 -0.0 143,978 - GFF423 0.27 -0.1 143,978 - GFF423 0.27 -0.0 143,978 - GFF423 0.27 +0.1 143,980 - GFF423 0.27 -0.2 144,336 - GFF423 0.50 +0.3 144,392 - GFF423 0.54 -0.6 144,392 - GFF423 0.54 +0.5 144,470 - GFF423 0.59 -0.1 144,487 + GFF423 0.60 +0.1 144,487 + GFF423 0.60 +2.1 144,488 - GFF423 0.60 -0.8 144,525 - GFF423 0.62 +0.3 144,595 + GFF423 0.67 -0.0 144,647 + GFF423 0.70 +0.7 144,648 - GFF423 0.70 -0.2 144,649 + GFF423 0.70 -0.3 144,721 + GFF423 0.75 +0.6 144,734 + GFF423 0.76 -0.2 144,746 - GFF423 0.77 +0.1 144,769 + GFF423 0.78 +0.3 144,824 - GFF423 0.82 -0.5 144,960 - +1.0 144,998 - -0.1 145,167 - -0.8 145,333 - +0.4 145,403 - +0.2 145,466 - GFF424 0.12 +0.1 145,567 - GFF424 0.18 +0.2 145,718 + GFF424 0.25 +0.1 145,785 + GFF424 0.29 -1.5 145,851 + GFF424 0.32 -0.3 145,852 - GFF424 0.32 -0.1 145,852 - GFF424 0.32 -0.8 145,852 - GFF424 0.32 +0.2 145,855 + GFF424 0.32 +0.8 145,856 - GFF424 0.32 -0.1 145,863 + GFF424 0.33 -0.2 145,864 - GFF424 0.33 +0.5 145,893 + GFF424 0.34 +0.2 145,894 - GFF424 0.34 -0.4 146,034 + GFF424 0.41 -0.3 146,062 + GFF424 0.43 -0.2 146,063 - GFF424 0.43 +0.3
Or see this region's nucleotide sequence