Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4186

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4185 and GFF4186 are separated by 325 nucleotidesGFF4186 and GFF4187 are separated by 11 nucleotides GFF4185 - hypothetical protein, at 86 to 208 GFF4185 GFF4186 - IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3), at 534 to 2,123 GFF4186 GFF4187 - Phosphoribosylamine--glycine ligase (EC 6.3.4.13), at 2,135 to 3,424 GFF4187 Position (kb) 0 1 2 3Strain fitness (log2 ratio) -1 0 1at 0.307 kb on + strandat 0.468 kb on + strandat 0.469 kb on - strandat 0.508 kb on + strandat 0.508 kb on + strandat 0.509 kb on - strandat 0.530 kb on + strandat 0.825 kb on - strand, within GFF4186at 0.947 kb on + strand, within GFF4186at 1.005 kb on - strand, within GFF4186at 1.019 kb on + strand, within GFF4186at 1.019 kb on + strand, within GFF4186at 1.020 kb on - strand, within GFF4186at 1.110 kb on + strand, within GFF4186at 1.111 kb on - strand, within GFF4186at 1.249 kb on - strand, within GFF4186at 1.249 kb on - strand, within GFF4186at 1.249 kb on - strand, within GFF4186at 1.252 kb on + strand, within GFF4186at 1.318 kb on - strand, within GFF4186at 1.444 kb on - strand, within GFF4186at 1.536 kb on - strand, within GFF4186at 1.748 kb on + strand, within GFF4186at 1.833 kb on + strand, within GFF4186at 1.985 kb on + strandat 2.143 kb on - strandat 2.290 kb on + strand, within GFF4187at 2.926 kb on + strand, within GFF4187at 2.927 kb on - strand, within GFF4187at 2.930 kb on + strand, within GFF4187at 2.931 kb on - strand, within GFF4187at 3.036 kb on + strand, within GFF4187at 3.036 kb on + strand, within GFF4187at 3.036 kb on + strand, within GFF4187at 3.037 kb on - strand, within GFF4187

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI
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307 + -0.4
468 + -0.1
469 - -0.3
508 + +0.1
508 + -0.5
509 - +0.3
530 + +0.5
825 - GFF4186 0.18 +0.2
947 + GFF4186 0.26 +0.8
1,005 - GFF4186 0.30 +0.7
1,019 + GFF4186 0.31 -0.8
1,019 + GFF4186 0.31 +0.6
1,020 - GFF4186 0.31 +0.5
1,110 + GFF4186 0.36 +1.0
1,111 - GFF4186 0.36 +0.2
1,249 - GFF4186 0.45 +0.8
1,249 - GFF4186 0.45 +0.9
1,249 - GFF4186 0.45 +1.0
1,252 + GFF4186 0.45 +0.6
1,318 - GFF4186 0.49 +1.1
1,444 - GFF4186 0.57 +1.1
1,536 - GFF4186 0.63 +1.3
1,748 + GFF4186 0.76 +0.6
1,833 + GFF4186 0.82 +1.2
1,985 + +0.5
2,143 - +0.7
2,290 + GFF4187 0.12 +0.1
2,926 + GFF4187 0.61 +0.7
2,927 - GFF4187 0.61 +0.6
2,930 + GFF4187 0.62 +0.6
2,931 - GFF4187 0.62 +0.4
3,036 + GFF4187 0.70 +1.0
3,036 + GFF4187 0.70 +0.4
3,036 + GFF4187 0.70 +0.7
3,037 - GFF4187 0.70 +1.0

Or see this region's nucleotide sequence