Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4099 and GFF4100 are separated by 108 nucleotides GFF4100 and GFF4101 are separated by 239 nucleotides
GFF4099 - Aliphatic amidase AmiE (EC 3.5.1.4), at 57,131 to 57,898
GFF4099
GFF4100 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 58,007 to 60,451
GFF4100
GFF4101 - Phosphoheptose isomerase 1 (EC 5.3.1.-), at 60,691 to 61,269
GFF4101
Position (kb) 58
59
60
61 Strain fitness (log2 ratio) -1
0
1
2 at 57.043 kb on + strand at 57.061 kb on - strand at 57.061 kb on - strand at 57.133 kb on - strand at 57.181 kb on - strand at 57.291 kb on + strand, within GFF4099 at 57.371 kb on + strand, within GFF4099 at 57.414 kb on - strand, within GFF4099 at 57.417 kb on + strand, within GFF4099 at 57.418 kb on - strand, within GFF4099 at 57.418 kb on - strand, within GFF4099 at 57.433 kb on - strand, within GFF4099 at 57.581 kb on - strand, within GFF4099 at 57.936 kb on - strand at 57.936 kb on - strand at 57.936 kb on - strand at 57.936 kb on - strand at 57.936 kb on - strand at 57.936 kb on - strand at 58.145 kb on - strand at 58.149 kb on - strand at 58.150 kb on + strand at 58.150 kb on + strand at 58.195 kb on + strand at 58.281 kb on - strand, within GFF4100 at 58.358 kb on + strand, within GFF4100 at 58.358 kb on + strand, within GFF4100 at 58.359 kb on - strand, within GFF4100 at 58.388 kb on - strand, within GFF4100 at 58.596 kb on + strand, within GFF4100 at 58.597 kb on - strand, within GFF4100 at 58.725 kb on + strand, within GFF4100 at 58.726 kb on - strand, within GFF4100 at 58.843 kb on + strand, within GFF4100 at 58.844 kb on - strand, within GFF4100 at 58.844 kb on - strand, within GFF4100 at 58.865 kb on - strand, within GFF4100 at 58.893 kb on - strand, within GFF4100 at 58.896 kb on + strand, within GFF4100 at 58.896 kb on + strand, within GFF4100 at 58.897 kb on - strand, within GFF4100 at 58.897 kb on - strand, within GFF4100 at 58.897 kb on - strand, within GFF4100 at 59.026 kb on + strand, within GFF4100 at 59.095 kb on - strand, within GFF4100 at 59.137 kb on + strand, within GFF4100 at 59.137 kb on + strand, within GFF4100 at 59.146 kb on - strand, within GFF4100 at 59.216 kb on - strand, within GFF4100 at 59.327 kb on - strand, within GFF4100 at 59.398 kb on + strand, within GFF4100 at 59.398 kb on + strand, within GFF4100 at 59.400 kb on + strand, within GFF4100 at 59.400 kb on + strand, within GFF4100 at 59.407 kb on + strand, within GFF4100 at 59.408 kb on - strand, within GFF4100 at 59.598 kb on - strand, within GFF4100 at 59.617 kb on - strand, within GFF4100 at 59.805 kb on + strand, within GFF4100 at 59.805 kb on + strand, within GFF4100 at 60.109 kb on - strand, within GFF4100 at 60.170 kb on - strand, within GFF4100 at 60.384 kb on - strand at 60.436 kb on + strand at 60.437 kb on - strand at 60.500 kb on - strand at 60.634 kb on + strand at 60.952 kb on - strand, within GFF4101 at 61.223 kb on + strand at 61.224 kb on - strand at 61.271 kb on + strand at 61.272 kb on - strand at 61.273 kb on + strand at 61.273 kb on + strand at 61.335 kb on - strand at 61.338 kb on + strand at 61.397 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI remove 57,043 + +0.3 57,061 - +1.5 57,061 - +0.0 57,133 - +0.2 57,181 - +0.1 57,291 + GFF4099 0.21 -1.1 57,371 + GFF4099 0.31 +0.5 57,414 - GFF4099 0.37 +0.9 57,417 + GFF4099 0.37 -0.7 57,418 - GFF4099 0.37 -0.2 57,418 - GFF4099 0.37 +0.0 57,433 - GFF4099 0.39 -0.2 57,581 - GFF4099 0.59 -0.0 57,936 - +0.6 57,936 - +0.4 57,936 - +0.1 57,936 - +0.2 57,936 - -0.2 57,936 - +0.4 58,145 - +0.3 58,149 - -0.2 58,150 + +0.2 58,150 + -0.8 58,195 + -0.4 58,281 - GFF4100 0.11 +0.1 58,358 + GFF4100 0.14 +0.2 58,358 + GFF4100 0.14 +0.1 58,359 - GFF4100 0.14 +0.0 58,388 - GFF4100 0.16 +0.3 58,596 + GFF4100 0.24 -0.5 58,597 - GFF4100 0.24 +0.8 58,725 + GFF4100 0.29 +0.4 58,726 - GFF4100 0.29 +0.4 58,843 + GFF4100 0.34 +0.2 58,844 - GFF4100 0.34 -0.1 58,844 - GFF4100 0.34 -0.2 58,865 - GFF4100 0.35 +0.4 58,893 - GFF4100 0.36 +0.8 58,896 + GFF4100 0.36 -0.2 58,896 + GFF4100 0.36 -1.4 58,897 - GFF4100 0.36 +0.1 58,897 - GFF4100 0.36 +0.2 58,897 - GFF4100 0.36 -0.2 59,026 + GFF4100 0.42 +0.3 59,095 - GFF4100 0.44 -0.0 59,137 + GFF4100 0.46 -1.3 59,137 + GFF4100 0.46 +0.2 59,146 - GFF4100 0.47 -0.3 59,216 - GFF4100 0.49 +0.4 59,327 - GFF4100 0.54 +0.2 59,398 + GFF4100 0.57 +0.5 59,398 + GFF4100 0.57 +1.6 59,400 + GFF4100 0.57 -0.4 59,400 + GFF4100 0.57 +1.0 59,407 + GFF4100 0.57 -0.1 59,408 - GFF4100 0.57 -0.1 59,598 - GFF4100 0.65 +0.5 59,617 - GFF4100 0.66 -0.3 59,805 + GFF4100 0.74 +0.6 59,805 + GFF4100 0.74 -0.3 60,109 - GFF4100 0.86 +0.3 60,170 - GFF4100 0.88 -0.0 60,384 - -0.3 60,436 + -0.0 60,437 - +0.0 60,500 - +0.1 60,634 + +2.6 60,952 - GFF4101 0.45 -1.0 61,223 + +0.6 61,224 - +1.6 61,271 + +0.6 61,272 - -0.4 61,273 + +0.8 61,273 + +0.9 61,335 - -0.2 61,338 + +1.5 61,397 - -0.5
Or see this region's nucleotide sequence