Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4020

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntGFF4018 and GFF4019 are separated by 107 nucleotidesGFF4019 and GFF4020 are separated by 46 nucleotidesGFF4020 and GFF4021 are separated by 119 nucleotidesGFF4021 and GFF4022 are separated by 297 nucleotides GFF4018 - Flagellar M-ring protein FliF, at 286,632 to 288,227 GFF4018 GFF4019 - FIG00640941: hypothetical protein, at 288,335 to 288,484 GFF4019 GFF4020 - Flagellar hook-basal body complex protein FliE, at 288,531 to 288,845 GFF4020 GFF4021 - FIG01200701: possible membrane protein, at 288,965 to 289,318 GFF4021 GFF4022 - UPF0033 protein YedF, at 289,616 to 289,849 GFF4022 Position (kb) 288 289Strain fitness (log2 ratio) -1 0 1 2 3at 287.997 kb on - strand, within GFF4018at 288.149 kb on + strandat 288.149 kb on + strandat 288.541 kb on + strandat 288.563 kb on + strand, within GFF4020at 288.572 kb on - strand, within GFF4020at 288.644 kb on - strand, within GFF4020at 288.644 kb on - strand, within GFF4020at 288.644 kb on - strand, within GFF4020at 288.891 kb on + strandat 289.181 kb on - strand, within GFF4021

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI
remove
287,997 - GFF4018 0.86 +2.9
288,149 + +1.3
288,149 + +1.6
288,541 + +1.6
288,563 + GFF4020 0.10 +2.2
288,572 - GFF4020 0.13 +1.7
288,644 - GFF4020 0.36 -0.6
288,644 - GFF4020 0.36 +1.4
288,644 - GFF4020 0.36 +1.9
288,891 + +0.4
289,181 - GFF4021 0.61 +0.2

Or see this region's nucleotide sequence